Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pth and ychH are separated by 277 nucleotides ychH and ychM are separated by 54 nucleotides ychM and prsA are separated by 124 nucleotides
b1204: pth - peptidyl-tRNA hydrolase (NCBI), at 1,257,152 to 1,257,736
pth
b1205: ychH - predicted inner membrane protein (NCBI), at 1,258,014 to 1,258,292
ychH
b1206: ychM - orf, hypothetical protein (VIMSS), at 1,258,347 to 1,260,026
ychM
b1207: prsA - ribose-phosphate pyrophosphokinase (NCBI), at 1,260,151 to 1,261,098
prsA
Position (kb)
1258
1259
1260
1261 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1257.756 kb on + strand at 1257.756 kb on + strand at 1257.756 kb on + strand at 1257.957 kb on + strand at 1258.135 kb on - strand, within ychH at 1258.207 kb on + strand, within ychH at 1258.341 kb on - strand at 1258.399 kb on + strand at 1258.434 kb on - strand at 1258.474 kb on - strand at 1258.636 kb on - strand, within ychM at 1258.636 kb on - strand, within ychM at 1258.716 kb on - strand, within ychM at 1258.716 kb on - strand, within ychM at 1258.860 kb on + strand, within ychM at 1259.080 kb on - strand, within ychM at 1259.080 kb on - strand, within ychM at 1259.171 kb on - strand, within ychM at 1259.178 kb on - strand, within ychM at 1259.255 kb on - strand, within ychM at 1259.273 kb on + strand, within ychM at 1259.362 kb on - strand, within ychM at 1259.391 kb on - strand, within ychM at 1259.391 kb on - strand, within ychM at 1259.426 kb on - strand, within ychM at 1259.505 kb on - strand, within ychM at 1259.661 kb on + strand, within ychM at 1259.743 kb on + strand, within ychM at 1259.743 kb on + strand, within ychM at 1259.797 kb on + strand, within ychM at 1259.808 kb on + strand, within ychM at 1259.808 kb on + strand, within ychM at 1259.900 kb on + strand at 1259.943 kb on - strand at 1260.002 kb on - strand at 1260.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3 remove 1,257,756 + +1.1 1,257,756 + -1.4 1,257,756 + -0.4 1,257,957 + -0.4 1,258,135 - ychH b1205 0.43 -0.5 1,258,207 + ychH b1205 0.69 -1.9 1,258,341 - -2.8 1,258,399 + -1.4 1,258,434 - +1.0 1,258,474 - -0.6 1,258,636 - ychM b1206 0.17 +0.3 1,258,636 - ychM b1206 0.17 -1.9 1,258,716 - ychM b1206 0.22 +1.1 1,258,716 - ychM b1206 0.22 -3.2 1,258,860 + ychM b1206 0.31 -0.8 1,259,080 - ychM b1206 0.44 -0.4 1,259,080 - ychM b1206 0.44 -3.6 1,259,171 - ychM b1206 0.49 +0.7 1,259,178 - ychM b1206 0.49 -0.6 1,259,255 - ychM b1206 0.54 +0.3 1,259,273 + ychM b1206 0.55 -0.9 1,259,362 - ychM b1206 0.60 -0.7 1,259,391 - ychM b1206 0.62 -2.4 1,259,391 - ychM b1206 0.62 +2.1 1,259,426 - ychM b1206 0.64 -2.9 1,259,505 - ychM b1206 0.69 -3.6 1,259,661 + ychM b1206 0.78 +0.3 1,259,743 + ychM b1206 0.83 -2.6 1,259,743 + ychM b1206 0.83 -1.8 1,259,797 + ychM b1206 0.86 -0.7 1,259,808 + ychM b1206 0.87 -2.6 1,259,808 + ychM b1206 0.87 -2.6 1,259,900 + +1.0 1,259,943 - +0.5 1,260,002 - +0.6 1,260,002 - -0.0
Or see this region's nucleotide sequence