Strain Fitness in Escherichia coli BW25113 around b0975

Experiment: T7_phage 0.00015625 MOI TC3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyaB and hyaC are separated by 18 nucleotideshyaC and hyaD overlap by 4 nucleotideshyaD and hyaE overlap by 4 nucleotideshyaE and hyaF overlap by 4 nucleotides b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270 hyaB b0974: hyaC - hydrogenase 1, b-type cytochrome subunit (NCBI), at 1,034,289 to 1,034,996 hyaC b0975: hyaD - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,034,993 to 1,035,580 hyaD b0976: hyaE - protein involved in processing of HyaA and HyaB proteins (NCBI), at 1,035,577 to 1,035,975 hyaE b0977: hyaF - protein involved in nickel incorporation into hydrogenase-1 proteins (NCBI), at 1,035,972 to 1,036,829 hyaF Position (kb) 1034 1035 1036Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1034.059 kb on + strand, within hyaBat 1034.059 kb on + strand, within hyaBat 1034.191 kb on + strandat 1034.236 kb on - strandat 1034.252 kb on - strandat 1034.276 kb on + strandat 1034.276 kb on + strandat 1034.319 kb on + strandat 1034.319 kb on + strandat 1034.408 kb on - strand, within hyaCat 1034.577 kb on - strand, within hyaCat 1034.720 kb on - strand, within hyaCat 1034.720 kb on - strand, within hyaCat 1034.890 kb on - strand, within hyaCat 1034.895 kb on - strand, within hyaCat 1034.940 kb on + strandat 1034.940 kb on + strandat 1034.945 kb on + strandat 1034.947 kb on - strandat 1035.102 kb on + strand, within hyaDat 1035.201 kb on + strand, within hyaDat 1035.201 kb on + strand, within hyaDat 1035.291 kb on - strand, within hyaDat 1035.291 kb on - strand, within hyaDat 1035.310 kb on + strand, within hyaDat 1035.437 kb on + strand, within hyaDat 1035.437 kb on + strand, within hyaDat 1035.495 kb on + strand, within hyaDat 1035.503 kb on - strand, within hyaDat 1035.503 kb on - strand, within hyaDat 1035.803 kb on + strand, within hyaEat 1035.813 kb on + strand, within hyaEat 1035.892 kb on + strand, within hyaEat 1035.910 kb on - strand, within hyaEat 1035.910 kb on - strand, within hyaEat 1035.990 kb on + strandat 1036.062 kb on - strand, within hyaFat 1036.216 kb on + strand, within hyaFat 1036.216 kb on + strand, within hyaFat 1036.275 kb on + strand, within hyaFat 1036.294 kb on + strand, within hyaFat 1036.394 kb on - strand, within hyaFat 1036.463 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3
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1,034,059 + hyaB b0973 0.88 -1.5
1,034,059 + hyaB b0973 0.88 -1.5
1,034,191 + -1.2
1,034,236 - -0.0
1,034,252 - +0.3
1,034,276 + -1.7
1,034,276 + -1.7
1,034,319 + +0.4
1,034,319 + +1.4
1,034,408 - hyaC b0974 0.17 +0.3
1,034,577 - hyaC b0974 0.41 -0.4
1,034,720 - hyaC b0974 0.61 +1.0
1,034,720 - hyaC b0974 0.61 +0.5
1,034,890 - hyaC b0974 0.85 -0.1
1,034,895 - hyaC b0974 0.86 +0.7
1,034,940 + +3.4
1,034,940 + -2.7
1,034,945 + -3.6
1,034,947 - -3.0
1,035,102 + hyaD b0975 0.19 +0.4
1,035,201 + hyaD b0975 0.35 -0.3
1,035,201 + hyaD b0975 0.35 -0.1
1,035,291 - hyaD b0975 0.51 -0.1
1,035,291 - hyaD b0975 0.51 +1.2
1,035,310 + hyaD b0975 0.54 +0.5
1,035,437 + hyaD b0975 0.76 -0.0
1,035,437 + hyaD b0975 0.76 -1.2
1,035,495 + hyaD b0975 0.85 +0.0
1,035,503 - hyaD b0975 0.87 +2.1
1,035,503 - hyaD b0975 0.87 +1.4
1,035,803 + hyaE b0976 0.57 -0.8
1,035,813 + hyaE b0976 0.59 -0.3
1,035,892 + hyaE b0976 0.79 -4.1
1,035,910 - hyaE b0976 0.83 -0.5
1,035,910 - hyaE b0976 0.83 -0.8
1,035,990 + -0.9
1,036,062 - hyaF b0977 0.10 -3.0
1,036,216 + hyaF b0977 0.28 -1.3
1,036,216 + hyaF b0977 0.28 -3.3
1,036,275 + hyaF b0977 0.35 -3.9
1,036,294 + hyaF b0977 0.38 -1.1
1,036,394 - hyaF b0977 0.49 +1.4
1,036,463 + -0.6

Or see this region's nucleotide sequence