Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gsiA and yliB are separated by 19 nucleotides yliB and yliC are separated by 17 nucleotides yliC and yliD are separated by 2 nucleotides
b0829: gsiA - fused predicted peptide transport subunits of ABC superfamily: ATP-binding components (RefSeq), at 866,743 to 868,614
gsiA
b0830: yliB - predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 868,634 to 870,172
yliB
b0831: yliC - predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI), at 870,190 to 871,110
yliC
b0832: yliD - predicted peptide transporter subunit: membrane component of ABC superfamily (NCBI), at 871,113 to 872,024
yliD
Position (kb) 868
869
870
871 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 867.697 kb on + strand, within gsiA at 867.724 kb on + strand, within gsiA at 867.753 kb on - strand, within gsiA at 867.850 kb on + strand, within gsiA at 867.888 kb on + strand, within gsiA at 868.088 kb on + strand, within gsiA at 868.103 kb on - strand, within gsiA at 868.155 kb on + strand, within gsiA at 868.155 kb on + strand, within gsiA at 868.444 kb on - strand at 868.444 kb on - strand at 868.447 kb on + strand at 868.475 kb on + strand at 868.643 kb on + strand at 868.649 kb on - strand at 868.649 kb on - strand at 868.654 kb on + strand at 868.814 kb on + strand, within yliB at 868.814 kb on + strand, within yliB at 868.864 kb on + strand, within yliB at 868.872 kb on - strand, within yliB at 868.872 kb on - strand, within yliB at 868.907 kb on - strand, within yliB at 868.907 kb on - strand, within yliB at 869.006 kb on + strand, within yliB at 869.006 kb on + strand, within yliB at 869.006 kb on - strand, within yliB at 869.006 kb on - strand, within yliB at 869.025 kb on - strand, within yliB at 869.062 kb on + strand, within yliB at 869.062 kb on + strand, within yliB at 869.184 kb on + strand, within yliB at 869.192 kb on - strand, within yliB at 869.437 kb on + strand, within yliB at 869.601 kb on + strand, within yliB at 869.614 kb on + strand, within yliB at 869.670 kb on + strand, within yliB at 869.681 kb on + strand, within yliB at 869.743 kb on - strand, within yliB at 869.748 kb on - strand, within yliB at 869.845 kb on + strand, within yliB at 869.853 kb on - strand, within yliB at 869.909 kb on + strand, within yliB at 870.102 kb on + strand at 870.136 kb on - strand at 870.209 kb on - strand at 870.209 kb on - strand at 870.274 kb on + strand at 870.347 kb on - strand, within yliC at 870.379 kb on - strand, within yliC at 870.411 kb on - strand, within yliC at 870.426 kb on + strand, within yliC at 870.590 kb on - strand, within yliC at 870.598 kb on - strand, within yliC at 870.796 kb on + strand, within yliC at 870.852 kb on - strand, within yliC at 870.883 kb on - strand, within yliC at 870.923 kb on + strand, within yliC at 871.104 kb on - strand at 871.104 kb on - strand at 871.111 kb on + strand at 871.111 kb on + strand at 871.168 kb on + strand at 871.168 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3 remove 867,697 + gsiA b0829 0.51 +1.7 867,724 + gsiA b0829 0.52 -0.2 867,753 - gsiA b0829 0.54 +2.3 867,850 + gsiA b0829 0.59 +0.0 867,888 + gsiA b0829 0.61 +1.0 868,088 + gsiA b0829 0.72 +0.0 868,103 - gsiA b0829 0.73 -2.1 868,155 + gsiA b0829 0.75 +1.2 868,155 + gsiA b0829 0.75 -1.9 868,444 - +2.1 868,444 - +0.6 868,447 + +0.2 868,475 + -0.2 868,643 + -2.4 868,649 - -0.3 868,649 - -0.7 868,654 + +0.9 868,814 + yliB b0830 0.12 -0.1 868,814 + yliB b0830 0.12 +0.2 868,864 + yliB b0830 0.15 -3.1 868,872 - yliB b0830 0.15 -3.8 868,872 - yliB b0830 0.15 -1.8 868,907 - yliB b0830 0.18 +0.3 868,907 - yliB b0830 0.18 +1.1 869,006 + yliB b0830 0.24 -3.8 869,006 + yliB b0830 0.24 +0.4 869,006 - yliB b0830 0.24 -1.1 869,006 - yliB b0830 0.24 -0.2 869,025 - yliB b0830 0.25 +0.2 869,062 + yliB b0830 0.28 -1.1 869,062 + yliB b0830 0.28 +0.8 869,184 + yliB b0830 0.36 -1.5 869,192 - yliB b0830 0.36 -3.4 869,437 + yliB b0830 0.52 +1.0 869,601 + yliB b0830 0.63 +0.6 869,614 + yliB b0830 0.64 +1.0 869,670 + yliB b0830 0.67 -0.9 869,681 + yliB b0830 0.68 -0.2 869,743 - yliB b0830 0.72 -1.0 869,748 - yliB b0830 0.72 -3.7 869,845 + yliB b0830 0.79 -2.1 869,853 - yliB b0830 0.79 -2.1 869,909 + yliB b0830 0.83 -1.6 870,102 + +0.2 870,136 - +0.5 870,209 - +1.6 870,209 - -1.1 870,274 + +0.7 870,347 - yliC b0831 0.17 -0.9 870,379 - yliC b0831 0.21 -0.5 870,411 - yliC b0831 0.24 +0.6 870,426 + yliC b0831 0.26 +0.9 870,590 - yliC b0831 0.43 +1.2 870,598 - yliC b0831 0.44 -1.4 870,796 + yliC b0831 0.66 +0.6 870,852 - yliC b0831 0.72 -1.8 870,883 - yliC b0831 0.75 -3.4 870,923 + yliC b0831 0.80 -2.8 871,104 - -0.2 871,104 - -0.2 871,111 + -1.8 871,111 + +0.3 871,168 + +1.6 871,168 + +1.8
Or see this region's nucleotide sequence