Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt allD and fdrA are separated by 316 nucleotides fdrA and ylbE are separated by 9 nucleotides
b0517: allD - ureidoglycolate dehydrogenase (NCBI), at 544,538 to 545,587
allD
b0518: fdrA - membrane protein FdrA (NCBI), at 545,904 to 547,571
fdrA
b4572: ylbE - no description, at 547,581 to 548,839
ylbE
Position (kb) 545
546
547
548 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 544.914 kb on - strand, within allD at 544.937 kb on - strand, within allD at 544.939 kb on + strand, within allD at 544.940 kb on + strand, within allD at 544.947 kb on + strand, within allD at 544.950 kb on - strand, within allD at 544.982 kb on - strand, within allD at 544.997 kb on - strand, within allD at 544.997 kb on - strand, within allD at 545.028 kb on - strand, within allD at 545.045 kb on + strand, within allD at 545.144 kb on + strand, within allD at 545.203 kb on + strand, within allD at 545.218 kb on - strand, within allD at 545.245 kb on - strand, within allD at 545.312 kb on - strand, within allD at 545.327 kb on + strand, within allD at 545.395 kb on - strand, within allD at 545.430 kb on + strand, within allD at 545.430 kb on + strand, within allD at 545.469 kb on + strand, within allD at 545.481 kb on + strand, within allD at 545.481 kb on + strand, within allD at 545.499 kb on + strand at 545.505 kb on + strand at 545.505 kb on + strand at 545.505 kb on + strand at 545.505 kb on + strand at 545.513 kb on + strand at 545.561 kb on + strand at 545.568 kb on + strand at 545.606 kb on + strand at 545.609 kb on + strand at 545.611 kb on + strand at 545.611 kb on + strand at 545.853 kb on + strand at 545.853 kb on + strand at 545.853 kb on + strand at 545.856 kb on - strand at 545.856 kb on - strand at 545.861 kb on - strand at 545.864 kb on - strand at 545.888 kb on + strand at 545.888 kb on + strand at 546.052 kb on - strand at 546.152 kb on - strand, within fdrA at 546.152 kb on - strand, within fdrA at 546.166 kb on + strand, within fdrA at 546.166 kb on + strand, within fdrA at 546.221 kb on + strand, within fdrA at 546.325 kb on + strand, within fdrA at 546.411 kb on + strand, within fdrA at 546.427 kb on + strand, within fdrA at 546.559 kb on + strand, within fdrA at 546.559 kb on + strand, within fdrA at 546.609 kb on + strand, within fdrA at 546.609 kb on + strand, within fdrA at 546.693 kb on + strand, within fdrA at 546.957 kb on + strand, within fdrA at 547.026 kb on + strand, within fdrA at 547.026 kb on + strand, within fdrA at 547.030 kb on - strand, within fdrA at 547.054 kb on - strand, within fdrA at 547.054 kb on - strand, within fdrA at 547.081 kb on + strand, within fdrA at 547.406 kb on + strand at 547.406 kb on + strand at 547.462 kb on + strand at 547.591 kb on - strand at 547.628 kb on - strand at 547.638 kb on + strand at 547.644 kb on - strand at 547.644 kb on - strand at 547.648 kb on + strand at 547.729 kb on - strand, within ylbE at 547.729 kb on - strand, within ylbE at 547.895 kb on - strand, within ylbE at 548.009 kb on + strand, within ylbE at 548.151 kb on - strand, within ylbE at 548.165 kb on + strand, within ylbE at 548.165 kb on + strand, within ylbE at 548.208 kb on - strand, within ylbE at 548.208 kb on - strand, within ylbE at 548.506 kb on + strand, within ylbE at 548.533 kb on + strand, within ylbE at 548.533 kb on + strand, within ylbE
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3 remove 544,914 - allD b0517 0.36 -2.4 544,937 - allD b0517 0.38 -0.9 544,939 + allD b0517 0.38 -1.0 544,940 + allD b0517 0.38 -0.1 544,947 + allD b0517 0.39 +2.3 544,950 - allD b0517 0.39 -3.0 544,982 - allD b0517 0.42 -0.0 544,997 - allD b0517 0.44 -3.7 544,997 - allD b0517 0.44 -1.4 545,028 - allD b0517 0.47 -1.4 545,045 + allD b0517 0.48 -0.1 545,144 + allD b0517 0.58 +0.8 545,203 + allD b0517 0.63 -1.0 545,218 - allD b0517 0.65 -1.2 545,245 - allD b0517 0.67 -3.6 545,312 - allD b0517 0.74 -1.8 545,327 + allD b0517 0.75 -0.0 545,395 - allD b0517 0.82 -3.5 545,430 + allD b0517 0.85 -1.8 545,430 + allD b0517 0.85 -3.4 545,469 + allD b0517 0.89 -2.6 545,481 + allD b0517 0.90 -1.5 545,481 + allD b0517 0.90 +1.6 545,499 + -0.6 545,505 + -3.2 545,505 + +2.1 545,505 + +0.5 545,505 + -2.5 545,513 + -2.1 545,561 + +0.2 545,568 + -0.1 545,606 + +1.6 545,609 + -1.1 545,611 + +0.2 545,611 + -1.3 545,853 + +1.6 545,853 + +1.0 545,853 + +2.6 545,856 - +1.6 545,856 - +1.6 545,861 - -0.0 545,864 - +2.6 545,888 + +3.7 545,888 + -0.4 546,052 - -1.3 546,152 - fdrA b0518 0.15 +0.6 546,152 - fdrA b0518 0.15 +2.3 546,166 + fdrA b0518 0.16 +1.5 546,166 + fdrA b0518 0.16 -1.2 546,221 + fdrA b0518 0.19 -3.5 546,325 + fdrA b0518 0.25 +0.6 546,411 + fdrA b0518 0.30 +0.4 546,427 + fdrA b0518 0.31 +1.9 546,559 + fdrA b0518 0.39 -1.9 546,559 + fdrA b0518 0.39 -1.6 546,609 + fdrA b0518 0.42 -1.2 546,609 + fdrA b0518 0.42 -2.2 546,693 + fdrA b0518 0.47 -4.1 546,957 + fdrA b0518 0.63 -3.0 547,026 + fdrA b0518 0.67 +1.0 547,026 + fdrA b0518 0.67 +0.7 547,030 - fdrA b0518 0.68 +1.1 547,054 - fdrA b0518 0.69 -2.2 547,054 - fdrA b0518 0.69 -0.4 547,081 + fdrA b0518 0.71 +1.9 547,406 + +0.8 547,406 + -1.0 547,462 + -1.5 547,591 - -2.5 547,628 - +0.7 547,638 + +0.5 547,644 - +0.4 547,644 - +0.6 547,648 + -0.1 547,729 - ylbE b4572 0.12 +0.8 547,729 - ylbE b4572 0.12 +0.4 547,895 - ylbE b4572 0.25 +0.1 548,009 + ylbE b4572 0.34 -2.0 548,151 - ylbE b4572 0.45 +1.5 548,165 + ylbE b4572 0.46 -0.9 548,165 + ylbE b4572 0.46 +0.8 548,208 - ylbE b4572 0.50 +0.2 548,208 - ylbE b4572 0.50 +1.4 548,506 + ylbE b4572 0.73 -0.4 548,533 + ylbE b4572 0.76 +0.4 548,533 + ylbE b4572 0.76 -3.1
Or see this region's nucleotide sequence