Experiment: T7_phage 0.00015625 MOI TC3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tauB and tauC overlap by 4 nucleotides tauC and tauD overlap by 4 nucleotides tauD and hemB are separated by 106 nucleotides
b0366: tauB - taurine transporter subunit (NCBI), at 385,431 to 386,198
tauB
b0367: tauC - taurine transporter subunit (NCBI), at 386,195 to 387,022
tauC
b0368: tauD - taurine dioxygenase (NCBI), at 387,019 to 387,870
tauD
b0369: hemB - 5-aminolevulinate dehydratase = porphobilinogen synthase (VIMSS), at 387,977 to 388,951
hemB
Position (kb) 387
388 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 386.055 kb on + strand, within tauB at 386.094 kb on + strand, within tauB at 386.245 kb on - strand at 386.275 kb on + strand at 386.297 kb on - strand, within tauC at 386.297 kb on - strand, within tauC at 386.532 kb on + strand, within tauC at 386.532 kb on + strand, within tauC at 386.562 kb on + strand, within tauC at 386.586 kb on - strand, within tauC at 386.729 kb on + strand, within tauC at 386.829 kb on - strand, within tauC at 386.958 kb on + strand at 387.043 kb on - strand at 387.266 kb on + strand, within tauD at 387.266 kb on + strand, within tauD at 387.410 kb on + strand, within tauD at 387.622 kb on + strand, within tauD at 387.649 kb on + strand, within tauD at 387.649 kb on + strand, within tauD at 387.669 kb on - strand, within tauD at 387.678 kb on + strand, within tauD at 387.775 kb on + strand, within tauD at 387.775 kb on + strand, within tauD at 387.783 kb on - strand, within tauD at 387.896 kb on + strand at 387.908 kb on + strand at 387.908 kb on + strand at 387.916 kb on - strand at 387.916 kb on - strand at 387.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC3 remove 386,055 + tauB b0366 0.81 +2.8 386,094 + tauB b0366 0.86 +0.3 386,245 - -0.4 386,275 + -4.1 386,297 - tauC b0367 0.12 -2.0 386,297 - tauC b0367 0.12 +0.6 386,532 + tauC b0367 0.41 +0.1 386,532 + tauC b0367 0.41 +1.1 386,562 + tauC b0367 0.44 +0.6 386,586 - tauC b0367 0.47 -2.3 386,729 + tauC b0367 0.64 -0.6 386,829 - tauC b0367 0.77 -1.5 386,958 + -1.3 387,043 - +1.0 387,266 + tauD b0368 0.29 -2.6 387,266 + tauD b0368 0.29 -0.8 387,410 + tauD b0368 0.46 -0.1 387,622 + tauD b0368 0.71 -1.6 387,649 + tauD b0368 0.74 -1.0 387,649 + tauD b0368 0.74 -1.4 387,669 - tauD b0368 0.76 +0.8 387,678 + tauD b0368 0.77 -2.6 387,775 + tauD b0368 0.89 -3.6 387,775 + tauD b0368 0.89 +0.4 387,783 - tauD b0368 0.90 -3.0 387,896 + -2.5 387,908 + +0.0 387,908 + -1.5 387,916 - -0.3 387,916 - -2.6 387,916 - -2.8
Or see this region's nucleotide sequence