Experiment: T7_phage 0.00015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycaR and kdsB overlap by 4 nucleotides kdsB and ycbJ are separated by 153 nucleotides ycbJ and ycbC overlap by 24 nucleotides ycbC and smtA are separated by 135 nucleotides
b0917: ycaR - hypothetical protein (NCBI), at 969,896 to 970,078
ycaR
b0918: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase (NCBI), at 970,075 to 970,821
kdsB
b0919: ycbJ - hypothetical protein (NCBI), at 970,975 to 971,868
ycbJ
b0920: ycbC - conserved inner membrane protein (NCBI), at 971,845 to 972,624
ycbC
b0921: smtA - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 972,760 to 973,545
smtA
Position (kb) 970
971
972 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 970.868 kb on - strand at 970.868 kb on - strand at 971.000 kb on + strand at 971.061 kb on + strand at 971.071 kb on + strand, within ycbJ at 971.221 kb on - strand, within ycbJ at 971.291 kb on + strand, within ycbJ at 971.291 kb on + strand, within ycbJ at 971.300 kb on + strand, within ycbJ at 971.300 kb on + strand, within ycbJ at 971.348 kb on - strand, within ycbJ at 971.348 kb on - strand, within ycbJ at 971.350 kb on + strand, within ycbJ at 971.382 kb on + strand, within ycbJ at 971.382 kb on + strand, within ycbJ at 971.426 kb on + strand, within ycbJ at 971.434 kb on - strand, within ycbJ at 971.436 kb on + strand, within ycbJ at 971.442 kb on + strand, within ycbJ at 971.442 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.447 kb on + strand, within ycbJ at 971.706 kb on + strand, within ycbJ at 972.023 kb on - strand, within ycbC at 972.065 kb on - strand, within ycbC at 972.215 kb on - strand, within ycbC at 972.313 kb on - strand, within ycbC at 972.351 kb on - strand, within ycbC at 972.354 kb on + strand, within ycbC at 972.354 kb on + strand, within ycbC at 972.535 kb on + strand, within ycbC at 972.548 kb on - strand at 972.819 kb on + strand at 972.852 kb on + strand, within smtA
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.00015625 MOI TC2 remove 970,868 - -0.8 970,868 - -0.6 971,000 + +1.0 971,061 + +2.1 971,071 + ycbJ b0919 0.11 +0.9 971,221 - ycbJ b0919 0.28 -0.9 971,291 + ycbJ b0919 0.35 +0.4 971,291 + ycbJ b0919 0.35 +0.8 971,300 + ycbJ b0919 0.36 +1.8 971,300 + ycbJ b0919 0.36 +0.3 971,348 - ycbJ b0919 0.42 +0.5 971,348 - ycbJ b0919 0.42 -0.3 971,350 + ycbJ b0919 0.42 +1.6 971,382 + ycbJ b0919 0.46 +1.9 971,382 + ycbJ b0919 0.46 +1.3 971,426 + ycbJ b0919 0.50 +0.7 971,434 - ycbJ b0919 0.51 -1.0 971,436 + ycbJ b0919 0.52 +1.9 971,442 + ycbJ b0919 0.52 +0.8 971,442 + ycbJ b0919 0.52 +1.4 971,447 + ycbJ b0919 0.53 +0.4 971,447 + ycbJ b0919 0.53 +1.8 971,447 + ycbJ b0919 0.53 +0.6 971,706 + ycbJ b0919 0.82 +1.5 972,023 - ycbC b0920 0.23 +2.3 972,065 - ycbC b0920 0.28 +2.3 972,215 - ycbC b0920 0.47 +1.8 972,313 - ycbC b0920 0.60 +2.9 972,351 - ycbC b0920 0.65 +3.0 972,354 + ycbC b0920 0.65 +3.9 972,354 + ycbC b0920 0.65 +1.6 972,535 + ycbC b0920 0.88 +1.0 972,548 - +2.4 972,819 + -1.2 972,852 + smtA b0921 0.12 +0.2
Or see this region's nucleotide sequence