Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cstA and ybdD are separated by 182 nucleotides ybdD and ybdH are separated by 9 nucleotides
b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222
cstA
b4512: ybdD - hypothetical protein (NCBI), at 631,405 to 631,602
ybdD
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
Position (kb) 631
632 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 630.485 kb on + strand, within cstA at 630.486 kb on - strand, within cstA at 630.493 kb on - strand, within cstA at 630.511 kb on + strand, within cstA at 630.583 kb on + strand, within cstA at 630.640 kb on + strand, within cstA at 630.703 kb on + strand, within cstA at 630.766 kb on + strand, within cstA at 630.816 kb on + strand, within cstA at 630.834 kb on - strand, within cstA at 630.834 kb on - strand, within cstA at 630.853 kb on - strand, within cstA at 630.865 kb on + strand, within cstA at 630.865 kb on + strand, within cstA at 630.894 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 631.000 kb on + strand, within cstA at 631.000 kb on + strand, within cstA at 631.003 kb on + strand, within cstA at 631.068 kb on + strand at 631.068 kb on + strand at 631.126 kb on + strand at 631.180 kb on - strand at 631.194 kb on + strand at 631.194 kb on + strand at 631.236 kb on - strand at 631.250 kb on + strand at 631.262 kb on + strand at 631.262 kb on + strand at 631.264 kb on + strand at 631.278 kb on - strand at 631.300 kb on - strand at 631.300 kb on - strand at 631.305 kb on + strand at 631.398 kb on + strand at 631.398 kb on + strand at 631.401 kb on - strand at 631.413 kb on - strand at 631.413 kb on - strand at 631.780 kb on - strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH at 632.399 kb on + strand, within ybdH at 632.466 kb on - strand, within ybdH at 632.581 kb on + strand, within ybdH
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 630,485 + cstA b0598 0.65 -0.6 630,486 - cstA b0598 0.65 +1.0 630,493 - cstA b0598 0.65 +2.0 630,511 + cstA b0598 0.66 -0.8 630,583 + cstA b0598 0.70 -1.7 630,640 + cstA b0598 0.72 -2.9 630,703 + cstA b0598 0.75 -0.5 630,766 + cstA b0598 0.78 -1.1 630,816 + cstA b0598 0.81 +0.1 630,834 - cstA b0598 0.82 -2.4 630,834 - cstA b0598 0.82 -0.2 630,853 - cstA b0598 0.82 -0.1 630,865 + cstA b0598 0.83 -2.7 630,865 + cstA b0598 0.83 +0.7 630,894 - cstA b0598 0.84 +0.6 630,927 - cstA b0598 0.86 -0.6 630,927 - cstA b0598 0.86 -2.2 631,000 + cstA b0598 0.89 +0.7 631,000 + cstA b0598 0.89 +0.0 631,003 + cstA b0598 0.90 -1.5 631,068 + -3.3 631,068 + +1.4 631,126 + +1.2 631,180 - -1.0 631,194 + -2.4 631,194 + +1.9 631,236 - -3.5 631,250 + -2.3 631,262 + -1.6 631,262 + -0.9 631,264 + +0.1 631,278 - +1.1 631,300 - -0.1 631,300 - -1.3 631,305 + -2.4 631,398 + -1.4 631,398 + -1.1 631,401 - +4.0 631,413 - +0.8 631,413 - +0.4 631,780 - ybdH b0599 0.15 -1.3 631,782 + ybdH b0599 0.16 +0.2 631,782 + ybdH b0599 0.16 -2.7 631,825 + ybdH b0599 0.20 -0.6 631,828 - ybdH b0599 0.20 -3.8 631,828 - ybdH b0599 0.20 -1.2 631,833 - ybdH b0599 0.20 +2.0 631,833 - ybdH b0599 0.20 -1.1 631,926 + ybdH b0599 0.29 -0.4 631,926 - ybdH b0599 0.29 -2.8 632,035 + ybdH b0599 0.39 -0.1 632,048 + ybdH b0599 0.40 +1.2 632,147 + ybdH b0599 0.49 +0.1 632,160 + ybdH b0599 0.50 -0.6 632,399 + ybdH b0599 0.72 +0.1 632,466 - ybdH b0599 0.78 +0.1 632,581 + ybdH b0599 0.89 -0.1
Or see this region's nucleotide sequence