Strain Fitness in Escherichia coli BW25113 around b4199

Experiment: T7_phage 0.0015625 MOI TC2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntulaE and ulaF overlap by 1 nucleotidesulaF and yjfY are separated by 129 nucleotidesyjfY and rpsF are separated by 326 nucleotidesrpsF and priB are separated by 6 nucleotides b4197: ulaE - L-xylulose 5-phosphate 3-epimerase (NCBI), at 4,420,869 to 4,421,723 ulaE b4198: ulaF - L-ribulose 5-phosphate 4-epimerase (NCBI), at 4,421,723 to 4,422,409 ulaF b4199: yjfY - hypothetical protein (NCBI), at 4,422,539 to 4,422,814 yjfY b4200: rpsF - 30S ribosomal protein S6 (NCBI), at 4,423,141 to 4,423,536 rpsF b4201: priB - primosomal replication protein N (NCBI), at 4,423,543 to 4,423,857 priB Position (kb) 4422 4423Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4421.764 kb on - strandat 4421.811 kb on - strand, within ulaFat 4421.811 kb on - strand, within ulaFat 4421.866 kb on - strand, within ulaFat 4421.996 kb on + strand, within ulaFat 4421.996 kb on + strand, within ulaFat 4422.095 kb on - strand, within ulaFat 4422.095 kb on - strand, within ulaFat 4422.097 kb on + strand, within ulaFat 4422.133 kb on - strand, within ulaFat 4422.133 kb on - strand, within ulaFat 4422.188 kb on - strand, within ulaFat 4422.188 kb on - strand, within ulaFat 4422.212 kb on - strand, within ulaFat 4422.212 kb on - strand, within ulaFat 4422.229 kb on + strand, within ulaFat 4422.229 kb on + strand, within ulaFat 4422.321 kb on + strand, within ulaFat 4422.321 kb on + strand, within ulaFat 4422.326 kb on + strand, within ulaFat 4422.326 kb on + strand, within ulaFat 4422.331 kb on + strand, within ulaFat 4422.337 kb on - strand, within ulaFat 4422.403 kb on + strandat 4422.439 kb on + strandat 4422.447 kb on - strandat 4422.450 kb on + strandat 4422.458 kb on - strandat 4422.458 kb on - strandat 4422.486 kb on + strandat 4422.508 kb on - strandat 4422.527 kb on - strandat 4422.533 kb on - strandat 4422.543 kb on - strandat 4422.565 kb on + strandat 4422.568 kb on - strand, within yjfYat 4422.592 kb on - strand, within yjfYat 4422.598 kb on + strand, within yjfYat 4422.598 kb on + strand, within yjfYat 4422.606 kb on + strand, within yjfYat 4422.606 kb on + strand, within yjfYat 4422.608 kb on + strand, within yjfYat 4422.608 kb on + strand, within yjfYat 4422.608 kb on + strand, within yjfYat 4422.614 kb on - strand, within yjfYat 4422.614 kb on - strand, within yjfYat 4422.616 kb on - strand, within yjfYat 4422.616 kb on - strand, within yjfYat 4422.616 kb on - strand, within yjfYat 4422.616 kb on - strand, within yjfYat 4422.616 kb on - strand, within yjfYat 4422.616 kb on - strand, within yjfYat 4422.626 kb on - strand, within yjfYat 4422.642 kb on + strand, within yjfYat 4422.642 kb on + strand, within yjfYat 4422.655 kb on - strand, within yjfYat 4422.660 kb on - strand, within yjfYat 4422.660 kb on - strand, within yjfYat 4422.669 kb on - strand, within yjfYat 4422.687 kb on + strand, within yjfYat 4422.704 kb on + strand, within yjfYat 4422.704 kb on + strand, within yjfYat 4422.705 kb on - strand, within yjfYat 4422.771 kb on - strand, within yjfYat 4422.784 kb on + strand, within yjfYat 4422.799 kb on + strandat 4422.802 kb on - strandat 4422.812 kb on + strandat 4422.814 kb on - strandat 4422.820 kb on - strandat 4422.820 kb on - strandat 4422.855 kb on - strandat 4422.855 kb on - strandat 4423.415 kb on + strand, within rpsFat 4423.552 kb on + strandat 4423.552 kb on + strandat 4423.563 kb on + strandat 4423.605 kb on + strand, within priBat 4423.636 kb on + strand, within priBat 4423.736 kb on + strand, within priBat 4423.752 kb on + strand, within priB

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2
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4,421,764 - -4.0
4,421,811 - ulaF b4198 0.13 +1.7
4,421,811 - ulaF b4198 0.13 -2.8
4,421,866 - ulaF b4198 0.21 -3.1
4,421,996 + ulaF b4198 0.40 +0.2
4,421,996 + ulaF b4198 0.40 -2.5
4,422,095 - ulaF b4198 0.54 -3.9
4,422,095 - ulaF b4198 0.54 -4.4
4,422,097 + ulaF b4198 0.54 +0.3
4,422,133 - ulaF b4198 0.60 +1.1
4,422,133 - ulaF b4198 0.60 +0.3
4,422,188 - ulaF b4198 0.68 +0.1
4,422,188 - ulaF b4198 0.68 +0.4
4,422,212 - ulaF b4198 0.71 +0.8
4,422,212 - ulaF b4198 0.71 +2.5
4,422,229 + ulaF b4198 0.74 +0.6
4,422,229 + ulaF b4198 0.74 +0.7
4,422,321 + ulaF b4198 0.87 -2.1
4,422,321 + ulaF b4198 0.87 -1.3
4,422,326 + ulaF b4198 0.88 +0.9
4,422,326 + ulaF b4198 0.88 -0.7
4,422,331 + ulaF b4198 0.89 +0.2
4,422,337 - ulaF b4198 0.89 -0.2
4,422,403 + -0.6
4,422,439 + -0.9
4,422,447 - -3.4
4,422,450 + -0.6
4,422,458 - +0.3
4,422,458 - -2.2
4,422,486 + -4.4
4,422,508 - -1.1
4,422,527 - +1.4
4,422,533 - -1.8
4,422,543 - -0.8
4,422,565 + -3.2
4,422,568 - yjfY b4199 0.11 +2.2
4,422,592 - yjfY b4199 0.19 +0.2
4,422,598 + yjfY b4199 0.21 +1.9
4,422,598 + yjfY b4199 0.21 -2.9
4,422,606 + yjfY b4199 0.24 +1.0
4,422,606 + yjfY b4199 0.24 -0.2
4,422,608 + yjfY b4199 0.25 +1.2
4,422,608 + yjfY b4199 0.25 +1.2
4,422,608 + yjfY b4199 0.25 -2.6
4,422,614 - yjfY b4199 0.27 -2.3
4,422,614 - yjfY b4199 0.27 -3.0
4,422,616 - yjfY b4199 0.28 -2.0
4,422,616 - yjfY b4199 0.28 +2.4
4,422,616 - yjfY b4199 0.28 +1.4
4,422,616 - yjfY b4199 0.28 -2.8
4,422,616 - yjfY b4199 0.28 -2.9
4,422,616 - yjfY b4199 0.28 +0.7
4,422,626 - yjfY b4199 0.32 -0.8
4,422,642 + yjfY b4199 0.37 -2.0
4,422,642 + yjfY b4199 0.37 -0.7
4,422,655 - yjfY b4199 0.42 -1.7
4,422,660 - yjfY b4199 0.44 -3.0
4,422,660 - yjfY b4199 0.44 -1.1
4,422,669 - yjfY b4199 0.47 -3.2
4,422,687 + yjfY b4199 0.54 -1.4
4,422,704 + yjfY b4199 0.60 -2.3
4,422,704 + yjfY b4199 0.60 +0.5
4,422,705 - yjfY b4199 0.60 -1.6
4,422,771 - yjfY b4199 0.84 -0.8
4,422,784 + yjfY b4199 0.89 +0.9
4,422,799 + +0.2
4,422,802 - -2.6
4,422,812 + +1.0
4,422,814 - -0.5
4,422,820 - +1.5
4,422,820 - +0.8
4,422,855 - +0.2
4,422,855 - -0.6
4,423,415 + rpsF b4200 0.69 -1.1
4,423,552 + -0.3
4,423,552 + +1.5
4,423,563 + +0.5
4,423,605 + priB b4201 0.20 +2.2
4,423,636 + priB b4201 0.30 +2.0
4,423,736 + priB b4201 0.61 -0.1
4,423,752 + priB b4201 0.66 +0.3

Or see this region's nucleotide sequence