Strain Fitness in Escherichia coli BW25113 around b2886

Experiment: T7_phage 0.0015625 MOI TC2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygfQ and ygfS are separated by 35 nucleotidesygfS and ygfT overlap by 1 nucleotides b4464: ygfQ - predicted transporter (NCBI), at 3,025,143 to 3,026,510 ygfQ b2886: ygfS - putative oxidoreductase, Fe-S subunit (VIMSS), at 3,026,546 to 3,027,034 ygfS b2887: ygfT - putative oxidoreductase, Fe-S subunit (VIMSS), at 3,027,034 to 3,028,953 ygfT Position (kb) 3026 3027 3028Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3025.587 kb on - strand, within ygfQat 3025.589 kb on + strand, within ygfQat 3025.591 kb on + strand, within ygfQat 3025.599 kb on - strand, within ygfQat 3025.643 kb on + strand, within ygfQat 3025.755 kb on - strand, within ygfQat 3025.757 kb on - strand, within ygfQat 3025.863 kb on - strand, within ygfQat 3025.965 kb on - strand, within ygfQat 3025.965 kb on - strand, within ygfQat 3026.052 kb on + strand, within ygfQat 3026.088 kb on + strand, within ygfQat 3026.137 kb on + strand, within ygfQat 3026.137 kb on + strand, within ygfQat 3026.261 kb on - strandat 3026.261 kb on - strand, within ygfQat 3026.263 kb on + strand, within ygfQat 3026.321 kb on + strand, within ygfQat 3026.321 kb on + strand, within ygfQat 3026.331 kb on + strand, within ygfQat 3026.363 kb on - strand, within ygfQat 3026.414 kb on - strandat 3026.467 kb on - strandat 3026.623 kb on + strand, within ygfSat 3026.641 kb on + strand, within ygfSat 3026.662 kb on + strand, within ygfSat 3026.705 kb on + strand, within ygfSat 3026.723 kb on - strand, within ygfSat 3026.800 kb on - strand, within ygfSat 3026.800 kb on - strand, within ygfSat 3026.812 kb on - strand, within ygfSat 3026.853 kb on + strand, within ygfSat 3026.947 kb on + strand, within ygfSat 3026.947 kb on + strand, within ygfSat 3027.136 kb on - strandat 3027.136 kb on - strandat 3027.184 kb on - strandat 3027.255 kb on - strand, within ygfTat 3027.255 kb on - strand, within ygfTat 3027.337 kb on + strand, within ygfTat 3027.370 kb on - strand, within ygfTat 3027.380 kb on - strand, within ygfTat 3027.407 kb on + strand, within ygfTat 3027.466 kb on - strand, within ygfTat 3027.566 kb on + strand, within ygfTat 3027.574 kb on - strand, within ygfTat 3027.574 kb on - strand, within ygfTat 3027.635 kb on - strand, within ygfTat 3027.658 kb on + strand, within ygfTat 3027.676 kb on - strand, within ygfTat 3027.676 kb on - strand, within ygfTat 3027.687 kb on + strand, within ygfTat 3027.690 kb on - strand, within ygfTat 3027.720 kb on - strand, within ygfTat 3027.759 kb on - strand, within ygfTat 3027.870 kb on - strand, within ygfTat 3027.870 kb on - strand, within ygfTat 3027.929 kb on - strand, within ygfTat 3027.990 kb on - strand, within ygfTat 3027.990 kb on - strand, within ygfTat 3027.990 kb on - strand, within ygfT

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2
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3,025,587 - ygfQ b4464 0.32 -0.4
3,025,589 + ygfQ b4464 0.33 -0.2
3,025,591 + ygfQ b4464 0.33 +1.4
3,025,599 - ygfQ b4464 0.33 -0.8
3,025,643 + ygfQ b4464 0.37 +0.2
3,025,755 - ygfQ b4464 0.45 -2.4
3,025,757 - ygfQ b4464 0.45 +0.3
3,025,863 - ygfQ b4464 0.53 +1.2
3,025,965 - ygfQ b4464 0.60 -1.3
3,025,965 - ygfQ b4464 0.60 -1.0
3,026,052 + ygfQ b4464 0.66 +1.8
3,026,088 + ygfQ b4464 0.69 -2.4
3,026,137 + ygfQ b4464 0.73 -1.0
3,026,137 + ygfQ b4464 0.73 -2.9
3,026,261 - +1.7
3,026,261 - ygfQ b4464 0.82 -0.9
3,026,263 + ygfQ b4464 0.82 +1.5
3,026,321 + ygfQ b4464 0.86 +1.6
3,026,321 + ygfQ b4464 0.86 +1.2
3,026,331 + ygfQ b4464 0.87 -0.5
3,026,363 - ygfQ b4464 0.89 +1.1
3,026,414 - -1.3
3,026,467 - -1.8
3,026,623 + ygfS b2886 0.16 +1.6
3,026,641 + ygfS b2886 0.19 +1.0
3,026,662 + ygfS b2886 0.24 -1.5
3,026,705 + ygfS b2886 0.33 +2.4
3,026,723 - ygfS b2886 0.36 +0.8
3,026,800 - ygfS b2886 0.52 -0.5
3,026,800 - ygfS b2886 0.52 -0.3
3,026,812 - ygfS b2886 0.54 +0.7
3,026,853 + ygfS b2886 0.63 -3.5
3,026,947 + ygfS b2886 0.82 +2.2
3,026,947 + ygfS b2886 0.82 -2.8
3,027,136 - -3.6
3,027,136 - +0.5
3,027,184 - +0.6
3,027,255 - ygfT b2887 0.12 +0.5
3,027,255 - ygfT b2887 0.12 +1.7
3,027,337 + ygfT b2887 0.16 +1.1
3,027,370 - ygfT b2887 0.17 +0.6
3,027,380 - ygfT b2887 0.18 +1.2
3,027,407 + ygfT b2887 0.19 -0.6
3,027,466 - ygfT b2887 0.23 +1.6
3,027,566 + ygfT b2887 0.28 -1.9
3,027,574 - ygfT b2887 0.28 -2.2
3,027,574 - ygfT b2887 0.28 -0.2
3,027,635 - ygfT b2887 0.31 -3.4
3,027,658 + ygfT b2887 0.33 -1.8
3,027,676 - ygfT b2887 0.33 +0.2
3,027,676 - ygfT b2887 0.33 -2.3
3,027,687 + ygfT b2887 0.34 -1.0
3,027,690 - ygfT b2887 0.34 +0.0
3,027,720 - ygfT b2887 0.36 +0.4
3,027,759 - ygfT b2887 0.38 -1.1
3,027,870 - ygfT b2887 0.44 +1.7
3,027,870 - ygfT b2887 0.44 +0.4
3,027,929 - ygfT b2887 0.47 +0.9
3,027,990 - ygfT b2887 0.50 -0.4
3,027,990 - ygfT b2887 0.50 +0.5
3,027,990 - ygfT b2887 0.50 +0.0

Or see this region's nucleotide sequence