Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbJ and ygbK overlap by 4 nucleotides ygbK and ygbL are separated by 91 nucleotides ygbL and ygbM are separated by 4 nucleotides
b2736: ygbJ - predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,859,452 to 2,860,360
ygbJ
b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523
ygbK
b2738: ygbL - hypothetical protein (NCBI), at 2,861,615 to 2,862,253
ygbL
b2739: ygbM - hypothetical protein (NCBI), at 2,862,258 to 2,863,034
ygbM
Position (kb)
2860
2861
2862 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2859.528 kb on - strand at 2859.528 kb on - strand at 2859.539 kb on - strand at 2859.664 kb on + strand, within ygbJ at 2859.852 kb on - strand, within ygbJ at 2859.962 kb on - strand, within ygbJ at 2859.965 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.296 kb on + strand at 2860.464 kb on - strand at 2860.540 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.657 kb on + strand, within ygbK at 2860.721 kb on + strand, within ygbK at 2860.847 kb on - strand, within ygbK at 2860.851 kb on + strand, within ygbK at 2860.895 kb on - strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.997 kb on + strand, within ygbK at 2860.998 kb on - strand, within ygbK at 2861.029 kb on - strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.080 kb on + strand, within ygbK at 2861.111 kb on + strand, within ygbK at 2861.166 kb on - strand, within ygbK at 2861.195 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.518 kb on + strand at 2861.531 kb on + strand at 2861.531 kb on + strand at 2861.539 kb on - strand at 2861.539 kb on - strand at 2861.551 kb on + strand at 2861.602 kb on - strand at 2861.614 kb on - strand at 2861.614 kb on - strand at 2861.719 kb on - strand, within ygbL at 2861.766 kb on - strand, within ygbL at 2861.778 kb on + strand, within ygbL at 2861.778 kb on + strand, within ygbL at 2861.832 kb on + strand, within ygbL at 2861.924 kb on - strand, within ygbL at 2861.984 kb on - strand, within ygbL at 2862.001 kb on + strand, within ygbL at 2862.001 kb on + strand, within ygbL at 2862.384 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.479 kb on - strand, within ygbM at 2862.511 kb on - strand, within ygbM
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 2,859,528 - +0.9 2,859,528 - +1.9 2,859,539 - -0.2 2,859,664 + ygbJ b2736 0.23 -0.7 2,859,852 - ygbJ b2736 0.44 -1.0 2,859,962 - ygbJ b2736 0.56 -0.6 2,859,965 + ygbJ b2736 0.56 -0.3 2,860,243 + ygbJ b2736 0.87 -1.5 2,860,243 + ygbJ b2736 0.87 -2.3 2,860,296 + +0.1 2,860,464 - -3.3 2,860,540 - ygbK b2737 0.16 +0.2 2,860,598 - ygbK b2737 0.21 +0.3 2,860,598 - ygbK b2737 0.21 -0.7 2,860,657 + ygbK b2737 0.26 -2.9 2,860,721 + ygbK b2737 0.31 -1.2 2,860,847 - ygbK b2737 0.42 -1.9 2,860,851 + ygbK b2737 0.42 -0.6 2,860,895 - ygbK b2737 0.46 +2.4 2,860,990 + ygbK b2737 0.54 -2.3 2,860,990 + ygbK b2737 0.54 +1.2 2,860,997 + ygbK b2737 0.55 -1.5 2,860,998 - ygbK b2737 0.55 -0.1 2,861,029 - ygbK b2737 0.58 -1.3 2,861,044 + ygbK b2737 0.59 -2.7 2,861,044 + ygbK b2737 0.59 -2.6 2,861,080 + ygbK b2737 0.62 +0.7 2,861,111 + ygbK b2737 0.65 -2.5 2,861,166 - ygbK b2737 0.69 -2.8 2,861,195 + ygbK b2737 0.72 +0.8 2,861,301 + ygbK b2737 0.81 -0.7 2,861,301 + ygbK b2737 0.81 -2.5 2,861,518 + -0.0 2,861,531 + -2.5 2,861,531 + -2.1 2,861,539 - +0.3 2,861,539 - +0.4 2,861,551 + -0.7 2,861,602 - +0.8 2,861,614 - +0.7 2,861,614 - +0.4 2,861,719 - ygbL b2738 0.16 -0.4 2,861,766 - ygbL b2738 0.24 -0.0 2,861,778 + ygbL b2738 0.26 -1.4 2,861,778 + ygbL b2738 0.26 -0.2 2,861,832 + ygbL b2738 0.34 +0.8 2,861,924 - ygbL b2738 0.48 -1.4 2,861,984 - ygbL b2738 0.58 +0.6 2,862,001 + ygbL b2738 0.60 -0.3 2,862,001 + ygbL b2738 0.60 +1.1 2,862,384 - ygbM b2739 0.16 -1.0 2,862,439 - ygbM b2739 0.23 -0.4 2,862,439 - ygbM b2739 0.23 +1.3 2,862,479 - ygbM b2739 0.28 +0.9 2,862,511 - ygbM b2739 0.33 -0.5
Or see this region's nucleotide sequence