Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yehI and yehK are separated by 60 nucleotides yehK and yehL are separated by 306 nucleotides yehL and yehM are separated by 10 nucleotides
b2118: yehI - hypothetical protein (NCBI), at 2,198,301 to 2,201,933
yehI
b4541: yehK - hypothetical protein (NCBI), at 2,201,994 to 2,202,311
yehK
b2119: yehL - orf, hypothetical protein (VIMSS), at 2,202,618 to 2,203,706
yehL
b2120: yehM - hypothetical protein (NCBI), at 2,203,717 to 2,205,996
yehM
Position (kb)
2202
2203
2204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2201.645 kb on - strand at 2201.709 kb on + strand at 2201.709 kb on + strand at 2201.746 kb on + strand at 2201.764 kb on - strand at 2201.833 kb on + strand at 2201.833 kb on + strand at 2201.838 kb on + strand at 2201.955 kb on - strand at 2202.021 kb on + strand at 2202.040 kb on + strand, within yehK at 2202.102 kb on + strand, within yehK at 2202.114 kb on + strand, within yehK at 2202.116 kb on + strand, within yehK at 2202.125 kb on + strand, within yehK at 2202.125 kb on + strand, within yehK at 2202.135 kb on + strand, within yehK at 2202.152 kb on + strand, within yehK at 2202.166 kb on + strand, within yehK at 2202.166 kb on + strand, within yehK at 2202.166 kb on + strand, within yehK at 2202.199 kb on + strand, within yehK at 2202.431 kb on + strand at 2202.437 kb on + strand at 2202.445 kb on + strand at 2202.445 kb on + strand at 2202.662 kb on + strand at 2202.662 kb on + strand at 2202.663 kb on - strand at 2202.663 kb on - strand at 2202.663 kb on - strand at 2202.663 kb on - strand at 2202.665 kb on - strand at 2202.665 kb on - strand at 2202.665 kb on - strand at 2202.668 kb on - strand at 2202.670 kb on - strand at 2202.675 kb on - strand at 2202.675 kb on - strand at 2202.714 kb on - strand at 2202.719 kb on - strand at 2202.733 kb on - strand, within yehL at 2202.733 kb on - strand, within yehL at 2202.751 kb on + strand, within yehL at 2202.790 kb on + strand, within yehL at 2202.790 kb on + strand, within yehL at 2202.840 kb on - strand, within yehL at 2202.843 kb on - strand, within yehL at 2202.951 kb on + strand, within yehL at 2202.951 kb on + strand, within yehL at 2202.994 kb on - strand, within yehL at 2202.994 kb on - strand, within yehL at 2203.056 kb on - strand, within yehL at 2203.110 kb on + strand, within yehL at 2203.120 kb on + strand, within yehL at 2203.372 kb on + strand, within yehL at 2203.392 kb on + strand, within yehL at 2203.392 kb on + strand, within yehL at 2203.456 kb on + strand, within yehL at 2203.473 kb on + strand, within yehL at 2203.473 kb on + strand, within yehL at 2203.508 kb on + strand, within yehL at 2203.508 kb on + strand, within yehL at 2203.511 kb on - strand, within yehL at 2203.548 kb on + strand, within yehL at 2203.563 kb on - strand, within yehL at 2203.693 kb on + strand at 2203.903 kb on + strand at 2203.903 kb on + strand at 2203.918 kb on + strand at 2204.037 kb on + strand, within yehM at 2204.045 kb on - strand, within yehM at 2204.166 kb on + strand at 2204.172 kb on - strand, within yehM at 2204.172 kb on - strand, within yehM at 2204.208 kb on - strand, within yehM at 2204.213 kb on + strand, within yehM at 2204.313 kb on - strand, within yehM at 2204.313 kb on - strand, within yehM at 2204.399 kb on + strand, within yehM at 2204.639 kb on + strand, within yehM at 2204.639 kb on + strand, within yehM at 2204.639 kb on - strand, within yehM at 2204.670 kb on - strand, within yehM
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 2,201,645 - +1.7 2,201,709 + +0.1 2,201,709 + +0.3 2,201,746 + +1.2 2,201,764 - +0.8 2,201,833 + -0.1 2,201,833 + +0.3 2,201,838 + +0.6 2,201,955 - -0.0 2,202,021 + +0.6 2,202,040 + yehK b4541 0.14 +4.2 2,202,102 + yehK b4541 0.34 -0.8 2,202,114 + yehK b4541 0.38 +1.8 2,202,116 + yehK b4541 0.38 +1.7 2,202,125 + yehK b4541 0.41 -1.2 2,202,125 + yehK b4541 0.41 +3.1 2,202,135 + yehK b4541 0.44 +0.2 2,202,152 + yehK b4541 0.50 +1.2 2,202,166 + yehK b4541 0.54 -1.9 2,202,166 + yehK b4541 0.54 -1.0 2,202,166 + yehK b4541 0.54 -0.0 2,202,199 + yehK b4541 0.64 +2.8 2,202,431 + -0.5 2,202,437 + +1.0 2,202,445 + +0.5 2,202,445 + +4.6 2,202,662 + +2.8 2,202,662 + +2.8 2,202,663 - -2.0 2,202,663 - -2.0 2,202,663 - -2.7 2,202,663 - -2.4 2,202,665 - -0.5 2,202,665 - -2.4 2,202,665 - -1.5 2,202,668 - +0.9 2,202,670 - -2.0 2,202,675 - +1.2 2,202,675 - -0.2 2,202,714 - -1.8 2,202,719 - -2.4 2,202,733 - yehL b2119 0.11 +2.1 2,202,733 - yehL b2119 0.11 +0.8 2,202,751 + yehL b2119 0.12 +2.8 2,202,790 + yehL b2119 0.16 +2.8 2,202,790 + yehL b2119 0.16 +0.8 2,202,840 - yehL b2119 0.20 +0.7 2,202,843 - yehL b2119 0.21 -3.2 2,202,951 + yehL b2119 0.31 +2.8 2,202,951 + yehL b2119 0.31 +1.8 2,202,994 - yehL b2119 0.35 -0.5 2,202,994 - yehL b2119 0.35 -2.4 2,203,056 - yehL b2119 0.40 -0.6 2,203,110 + yehL b2119 0.45 -3.2 2,203,120 + yehL b2119 0.46 -0.1 2,203,372 + yehL b2119 0.69 +0.6 2,203,392 + yehL b2119 0.71 -2.2 2,203,392 + yehL b2119 0.71 -2.4 2,203,456 + yehL b2119 0.77 -1.8 2,203,473 + yehL b2119 0.79 -1.4 2,203,473 + yehL b2119 0.79 -1.8 2,203,508 + yehL b2119 0.82 -3.5 2,203,508 + yehL b2119 0.82 +0.9 2,203,511 - yehL b2119 0.82 -1.8 2,203,548 + yehL b2119 0.85 -1.9 2,203,563 - yehL b2119 0.87 -0.0 2,203,693 + +0.4 2,203,903 + -0.3 2,203,903 + -0.1 2,203,918 + -2.3 2,204,037 + yehM b2120 0.14 -1.5 2,204,045 - yehM b2120 0.14 +0.2 2,204,166 + +1.1 2,204,172 - yehM b2120 0.20 -1.2 2,204,172 - yehM b2120 0.20 +0.2 2,204,208 - yehM b2120 0.22 +0.6 2,204,213 + yehM b2120 0.22 +1.2 2,204,313 - yehM b2120 0.26 -1.4 2,204,313 - yehM b2120 0.26 -0.4 2,204,399 + yehM b2120 0.30 -2.5 2,204,639 + yehM b2120 0.40 +0.1 2,204,639 + yehM b2120 0.40 -0.6 2,204,639 - yehM b2120 0.40 -0.4 2,204,670 - yehM b2120 0.42 +1.1
Or see this region's nucleotide sequence