Strain Fitness in Escherichia coli BW25113 around b1972

Experiment: T7_phage 0.0015625 MOI TC2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedY and yedZ are separated by 0 nucleotidesyedZ and yodA are separated by 256 nucleotides b1971: yedY - hypothetical protein (NCBI), at 2,037,502 to 2,038,506 yedY b1972: yedZ - hypothetical protein (NCBI), at 2,038,507 to 2,039,142 yedZ b1973: yodA - conserved metal-binding protein (NCBI), at 2,039,399 to 2,040,049 yodA Position (kb) 2038 2039 2040Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2037.623 kb on - strand, within yedYat 2037.623 kb on - strand, within yedYat 2037.648 kb on + strand, within yedYat 2037.689 kb on + strand, within yedYat 2037.689 kb on + strand, within yedYat 2037.889 kb on + strand, within yedYat 2037.918 kb on - strand, within yedYat 2037.940 kb on + strand, within yedYat 2038.260 kb on + strand, within yedYat 2038.260 kb on + strand, within yedYat 2038.260 kb on + strand, within yedYat 2038.273 kb on - strand, within yedYat 2038.370 kb on + strand, within yedYat 2038.378 kb on - strand, within yedYat 2038.378 kb on - strand, within yedYat 2038.704 kb on + strand, within yedZat 2038.748 kb on + strand, within yedZat 2038.750 kb on + strand, within yedZat 2038.787 kb on + strand, within yedZat 2038.787 kb on + strand, within yedZat 2038.951 kb on - strand, within yedZat 2038.951 kb on - strand, within yedZat 2038.989 kb on - strand, within yedZat 2039.002 kb on - strand, within yedZat 2039.037 kb on + strand, within yedZat 2039.052 kb on + strand, within yedZat 2039.052 kb on + strand, within yedZat 2039.211 kb on - strandat 2039.216 kb on - strandat 2039.429 kb on + strandat 2039.439 kb on + strandat 2039.439 kb on + strandat 2039.451 kb on + strandat 2039.472 kb on + strand, within yodAat 2039.479 kb on + strand, within yodAat 2039.479 kb on + strand, within yodAat 2039.487 kb on - strand, within yodAat 2039.499 kb on - strand, within yodAat 2039.499 kb on - strand, within yodAat 2039.569 kb on + strand, within yodAat 2039.569 kb on - strand, within yodAat 2039.572 kb on + strand, within yodAat 2039.589 kb on + strand, within yodAat 2039.627 kb on + strand, within yodAat 2039.736 kb on + strand, within yodAat 2039.808 kb on + strand, within yodAat 2040.005 kb on - strandat 2040.010 kb on - strandat 2040.015 kb on - strandat 2040.023 kb on + strandat 2040.033 kb on - strandat 2040.095 kb on - strandat 2040.095 kb on - strandat 2040.139 kb on + strandat 2040.142 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2
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2,037,623 - yedY b1971 0.12 +2.7
2,037,623 - yedY b1971 0.12 +0.1
2,037,648 + yedY b1971 0.15 +1.7
2,037,689 + yedY b1971 0.19 +1.1
2,037,689 + yedY b1971 0.19 +2.7
2,037,889 + yedY b1971 0.39 +1.0
2,037,918 - yedY b1971 0.41 +2.9
2,037,940 + yedY b1971 0.44 -2.8
2,038,260 + yedY b1971 0.75 -0.6
2,038,260 + yedY b1971 0.75 +1.0
2,038,260 + yedY b1971 0.75 +0.4
2,038,273 - yedY b1971 0.77 -1.2
2,038,370 + yedY b1971 0.86 +1.4
2,038,378 - yedY b1971 0.87 -1.2
2,038,378 - yedY b1971 0.87 -0.5
2,038,704 + yedZ b1972 0.31 -1.1
2,038,748 + yedZ b1972 0.38 +0.3
2,038,750 + yedZ b1972 0.38 +0.7
2,038,787 + yedZ b1972 0.44 +0.7
2,038,787 + yedZ b1972 0.44 +0.4
2,038,951 - yedZ b1972 0.70 +0.9
2,038,951 - yedZ b1972 0.70 +1.3
2,038,989 - yedZ b1972 0.76 -2.5
2,039,002 - yedZ b1972 0.78 +1.2
2,039,037 + yedZ b1972 0.83 +1.2
2,039,052 + yedZ b1972 0.86 -1.5
2,039,052 + yedZ b1972 0.86 +1.0
2,039,211 - -0.8
2,039,216 - +0.2
2,039,429 + +1.1
2,039,439 + -1.0
2,039,439 + +2.7
2,039,451 + +0.7
2,039,472 + yodA b1973 0.11 -2.0
2,039,479 + yodA b1973 0.12 -1.4
2,039,479 + yodA b1973 0.12 +1.2
2,039,487 - yodA b1973 0.14 +1.9
2,039,499 - yodA b1973 0.15 -0.3
2,039,499 - yodA b1973 0.15 +2.2
2,039,569 + yodA b1973 0.26 +0.4
2,039,569 - yodA b1973 0.26 +0.7
2,039,572 + yodA b1973 0.27 +2.7
2,039,589 + yodA b1973 0.29 +0.8
2,039,627 + yodA b1973 0.35 +0.4
2,039,736 + yodA b1973 0.52 +0.7
2,039,808 + yodA b1973 0.63 +1.7
2,040,005 - +2.7
2,040,010 - +2.7
2,040,015 - +2.7
2,040,023 + +1.1
2,040,033 - +2.7
2,040,095 - +2.3
2,040,095 - -0.6
2,040,139 + +2.7
2,040,142 + +2.7

Or see this region's nucleotide sequence