Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedY and yedZ are separated by 0 nucleotides yedZ and yodA are separated by 256 nucleotides
b1971: yedY - hypothetical protein (NCBI), at 2,037,502 to 2,038,506
yedY
b1972: yedZ - hypothetical protein (NCBI), at 2,038,507 to 2,039,142
yedZ
b1973: yodA - conserved metal-binding protein (NCBI), at 2,039,399 to 2,040,049
yodA
Position (kb)
2038
2039
2040 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2037.623 kb on - strand, within yedY at 2037.623 kb on - strand, within yedY at 2037.648 kb on + strand, within yedY at 2037.689 kb on + strand, within yedY at 2037.689 kb on + strand, within yedY at 2037.889 kb on + strand, within yedY at 2037.918 kb on - strand, within yedY at 2037.940 kb on + strand, within yedY at 2038.260 kb on + strand, within yedY at 2038.260 kb on + strand, within yedY at 2038.260 kb on + strand, within yedY at 2038.273 kb on - strand, within yedY at 2038.370 kb on + strand, within yedY at 2038.378 kb on - strand, within yedY at 2038.378 kb on - strand, within yedY at 2038.704 kb on + strand, within yedZ at 2038.748 kb on + strand, within yedZ at 2038.750 kb on + strand, within yedZ at 2038.787 kb on + strand, within yedZ at 2038.787 kb on + strand, within yedZ at 2038.951 kb on - strand, within yedZ at 2038.951 kb on - strand, within yedZ at 2038.989 kb on - strand, within yedZ at 2039.002 kb on - strand, within yedZ at 2039.037 kb on + strand, within yedZ at 2039.052 kb on + strand, within yedZ at 2039.052 kb on + strand, within yedZ at 2039.211 kb on - strand at 2039.216 kb on - strand at 2039.429 kb on + strand at 2039.439 kb on + strand at 2039.439 kb on + strand at 2039.451 kb on + strand at 2039.472 kb on + strand, within yodA at 2039.479 kb on + strand, within yodA at 2039.479 kb on + strand, within yodA at 2039.487 kb on - strand, within yodA at 2039.499 kb on - strand, within yodA at 2039.499 kb on - strand, within yodA at 2039.569 kb on + strand, within yodA at 2039.569 kb on - strand, within yodA at 2039.572 kb on + strand, within yodA at 2039.589 kb on + strand, within yodA at 2039.627 kb on + strand, within yodA at 2039.736 kb on + strand, within yodA at 2039.808 kb on + strand, within yodA at 2040.005 kb on - strand at 2040.010 kb on - strand at 2040.015 kb on - strand at 2040.023 kb on + strand at 2040.033 kb on - strand at 2040.095 kb on - strand at 2040.095 kb on - strand at 2040.139 kb on + strand at 2040.142 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 2,037,623 - yedY b1971 0.12 +2.7 2,037,623 - yedY b1971 0.12 +0.1 2,037,648 + yedY b1971 0.15 +1.7 2,037,689 + yedY b1971 0.19 +1.1 2,037,689 + yedY b1971 0.19 +2.7 2,037,889 + yedY b1971 0.39 +1.0 2,037,918 - yedY b1971 0.41 +2.9 2,037,940 + yedY b1971 0.44 -2.8 2,038,260 + yedY b1971 0.75 -0.6 2,038,260 + yedY b1971 0.75 +1.0 2,038,260 + yedY b1971 0.75 +0.4 2,038,273 - yedY b1971 0.77 -1.2 2,038,370 + yedY b1971 0.86 +1.4 2,038,378 - yedY b1971 0.87 -1.2 2,038,378 - yedY b1971 0.87 -0.5 2,038,704 + yedZ b1972 0.31 -1.1 2,038,748 + yedZ b1972 0.38 +0.3 2,038,750 + yedZ b1972 0.38 +0.7 2,038,787 + yedZ b1972 0.44 +0.7 2,038,787 + yedZ b1972 0.44 +0.4 2,038,951 - yedZ b1972 0.70 +0.9 2,038,951 - yedZ b1972 0.70 +1.3 2,038,989 - yedZ b1972 0.76 -2.5 2,039,002 - yedZ b1972 0.78 +1.2 2,039,037 + yedZ b1972 0.83 +1.2 2,039,052 + yedZ b1972 0.86 -1.5 2,039,052 + yedZ b1972 0.86 +1.0 2,039,211 - -0.8 2,039,216 - +0.2 2,039,429 + +1.1 2,039,439 + -1.0 2,039,439 + +2.7 2,039,451 + +0.7 2,039,472 + yodA b1973 0.11 -2.0 2,039,479 + yodA b1973 0.12 -1.4 2,039,479 + yodA b1973 0.12 +1.2 2,039,487 - yodA b1973 0.14 +1.9 2,039,499 - yodA b1973 0.15 -0.3 2,039,499 - yodA b1973 0.15 +2.2 2,039,569 + yodA b1973 0.26 +0.4 2,039,569 - yodA b1973 0.26 +0.7 2,039,572 + yodA b1973 0.27 +2.7 2,039,589 + yodA b1973 0.29 +0.8 2,039,627 + yodA b1973 0.35 +0.4 2,039,736 + yodA b1973 0.52 +0.7 2,039,808 + yodA b1973 0.63 +1.7 2,040,005 - +2.7 2,040,010 - +2.7 2,040,015 - +2.7 2,040,023 + +1.1 2,040,033 - +2.7 2,040,095 - +2.3 2,040,095 - -0.6 2,040,139 + +2.7 2,040,142 + +2.7
Or see this region's nucleotide sequence