Strain Fitness in Escherichia coli BW25113 around b1590

Experiment: T7_phage 0.0015625 MOI TC2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfF and ynfG are separated by 10 nucleotidesynfG and ynfH are separated by 1 nucleotidesynfH and b1591 are separated by 42 nucleotidesb1591 and b1592 are separated by 194 nucleotides b1588: ynfF - oxidoreductase subunit (RefSeq), at 1,658,580 to 1,661,003 ynfF b1589: ynfG - oxidoreductase, Fe-S subunit (NCBI), at 1,661,014 to 1,661,631 ynfG b1590: ynfH - oxidoreductase, membrane subunit (NCBI), at 1,661,633 to 1,662,487 ynfH b1591: b1591 - putative oxidoreductase component (VIMSS), at 1,662,530 to 1,663,144 b1591 b1592: b1592 - putative chloride channel (VIMSS), at 1,663,339 to 1,664,595 b1592 Position (kb) 1661 1662 1663Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1660.766 kb on + strandat 1660.766 kb on + strandat 1660.779 kb on - strandat 1660.779 kb on - strandat 1660.790 kb on - strandat 1661.057 kb on - strandat 1661.346 kb on - strand, within ynfGat 1661.351 kb on + strand, within ynfGat 1661.351 kb on + strand, within ynfGat 1661.364 kb on - strand, within ynfGat 1661.755 kb on + strand, within ynfHat 1661.755 kb on + strand, within ynfHat 1661.782 kb on - strand, within ynfHat 1661.851 kb on - strand, within ynfHat 1662.281 kb on + strand, within ynfHat 1662.281 kb on + strand, within ynfHat 1662.405 kb on + strandat 1662.482 kb on + strandat 1662.522 kb on + strandat 1662.589 kb on + strandat 1662.796 kb on + strand, within b1591at 1662.877 kb on + strand, within b1591

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2
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1,660,766 + -1.7
1,660,766 + +0.0
1,660,779 - -1.7
1,660,779 - +1.5
1,660,790 - -1.0
1,661,057 - -1.6
1,661,346 - ynfG b1589 0.54 +0.4
1,661,351 + ynfG b1589 0.55 -1.5
1,661,351 + ynfG b1589 0.55 -1.8
1,661,364 - ynfG b1589 0.57 -1.5
1,661,755 + ynfH b1590 0.14 -3.9
1,661,755 + ynfH b1590 0.14 -2.7
1,661,782 - ynfH b1590 0.17 -0.7
1,661,851 - ynfH b1590 0.25 -3.1
1,662,281 + ynfH b1590 0.76 -1.8
1,662,281 + ynfH b1590 0.76 -0.6
1,662,405 + -1.0
1,662,482 + -1.4
1,662,522 + -0.2
1,662,589 + +0.7
1,662,796 + b1591 b1591 0.43 -2.1
1,662,877 + b1591 b1591 0.56 -2.0

Or see this region's nucleotide sequence