Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b0868 and ybjT are separated by 98 nucleotides ybjT and ltaE are separated by 10 nucleotides
b0868: b0868 - putative nucleotide di-P-sugar epimerase or dehydratase (VIMSS), at 904,963 to 905,976
b0868
b0869: ybjT - putative dTDP-glucose enzyme (VIMSS), at 906,075 to 907,505
ybjT
b0870: ltaE - L-allo-threonine aldolase, PLP-dependent (NCBI), at 907,516 to 908,517
ltaE
Position (kb) 906
907
908 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 905.245 kb on + strand, within b0868 at 905.246 kb on - strand, within b0868 at 905.246 kb on - strand, within b0868 at 905.248 kb on + strand, within b0868 at 905.253 kb on + strand, within b0868 at 905.261 kb on - strand, within b0868 at 905.261 kb on - strand, within b0868 at 905.275 kb on - strand, within b0868 at 905.278 kb on + strand, within b0868 at 905.278 kb on + strand, within b0868 at 905.284 kb on - strand, within b0868 at 905.286 kb on - strand, within b0868 at 905.357 kb on - strand, within b0868 at 905.357 kb on - strand, within b0868 at 905.376 kb on - strand, within b0868 at 905.402 kb on + strand, within b0868 at 905.468 kb on + strand, within b0868 at 905.489 kb on - strand, within b0868 at 905.525 kb on - strand, within b0868 at 905.543 kb on + strand, within b0868 at 905.734 kb on + strand, within b0868 at 905.734 kb on + strand, within b0868 at 905.735 kb on + strand, within b0868 at 905.735 kb on + strand, within b0868 at 905.746 kb on + strand, within b0868 at 905.964 kb on + strand at 906.135 kb on - strand at 906.263 kb on - strand, within ybjT at 906.527 kb on + strand, within ybjT at 906.527 kb on + strand, within ybjT at 906.635 kb on - strand, within ybjT at 906.667 kb on - strand, within ybjT at 906.667 kb on - strand, within ybjT at 906.865 kb on - strand, within ybjT at 907.084 kb on + strand, within ybjT at 907.090 kb on + strand, within ybjT at 907.090 kb on + strand, within ybjT at 907.271 kb on + strand, within ybjT at 907.385 kb on + strand at 907.462 kb on + strand at 907.473 kb on - strand at 908.109 kb on + strand, within ltaE at 908.179 kb on - strand, within ltaE at 908.192 kb on - strand, within ltaE at 908.220 kb on + strand, within ltaE at 908.235 kb on + strand, within ltaE at 908.314 kb on + strand, within ltaE at 908.337 kb on + strand, within ltaE at 908.403 kb on - strand, within ltaE at 908.461 kb on - strand at 908.493 kb on + strand at 908.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 905,245 + b0868 b0868 0.28 -3.2 905,246 - b0868 b0868 0.28 -1.2 905,246 - b0868 b0868 0.28 -3.4 905,248 + b0868 b0868 0.28 -2.9 905,253 + b0868 b0868 0.29 +0.8 905,261 - b0868 b0868 0.29 -0.7 905,261 - b0868 b0868 0.29 -0.6 905,275 - b0868 b0868 0.31 -0.3 905,278 + b0868 b0868 0.31 +0.4 905,278 + b0868 b0868 0.31 -1.7 905,284 - b0868 b0868 0.32 -1.3 905,286 - b0868 b0868 0.32 +0.1 905,357 - b0868 b0868 0.39 -0.6 905,357 - b0868 b0868 0.39 +0.2 905,376 - b0868 b0868 0.41 -2.6 905,402 + b0868 b0868 0.43 -3.4 905,468 + b0868 b0868 0.50 -2.8 905,489 - b0868 b0868 0.52 -0.1 905,525 - b0868 b0868 0.55 -0.5 905,543 + b0868 b0868 0.57 -1.2 905,734 + b0868 b0868 0.76 -1.3 905,734 + b0868 b0868 0.76 +0.2 905,735 + b0868 b0868 0.76 -2.8 905,735 + b0868 b0868 0.76 -1.8 905,746 + b0868 b0868 0.77 -2.7 905,964 + +1.6 906,135 - -1.5 906,263 - ybjT b0869 0.13 -2.4 906,527 + ybjT b0869 0.32 -2.3 906,527 + ybjT b0869 0.32 -0.5 906,635 - ybjT b0869 0.39 -0.8 906,667 - ybjT b0869 0.41 +1.3 906,667 - ybjT b0869 0.41 +0.7 906,865 - ybjT b0869 0.55 -2.5 907,084 + ybjT b0869 0.71 -1.2 907,090 + ybjT b0869 0.71 -0.1 907,090 + ybjT b0869 0.71 +2.2 907,271 + ybjT b0869 0.84 -1.0 907,385 + -2.2 907,462 + +2.5 907,473 - -0.5 908,109 + ltaE b0870 0.59 +0.3 908,179 - ltaE b0870 0.66 +1.0 908,192 - ltaE b0870 0.67 -0.1 908,220 + ltaE b0870 0.70 -0.9 908,235 + ltaE b0870 0.72 -3.0 908,314 + ltaE b0870 0.80 +1.5 908,337 + ltaE b0870 0.82 -0.0 908,403 - ltaE b0870 0.89 -2.3 908,461 - -1.2 908,493 + -0.9 908,493 + -1.4
Or see this region's nucleotide sequence