Strain Fitness in Escherichia coli BW25113 around b0485

Experiment: T7_phage 0.0015625 MOI TC2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcopA and ybaS are separated by 261 nucleotidesybaS and ybaT are separated by 2 nucleotides b0484: copA - copper transporter (NCBI), at 508,099 to 510,603 copA b0485: ybaS - predicted glutaminase (NCBI), at 510,865 to 511,797 ybaS b0486: ybaT - predicted transporter (NCBI), at 511,800 to 513,092 ybaT Position (kb) 510 511 512Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 509.929 kb on + strand, within copAat 509.935 kb on + strand, within copAat 510.004 kb on - strand, within copAat 510.019 kb on + strand, within copAat 510.186 kb on + strand, within copAat 510.217 kb on - strand, within copAat 510.217 kb on - strand, within copAat 510.264 kb on - strand, within copAat 510.266 kb on + strand, within copAat 510.272 kb on + strand, within copAat 510.277 kb on + strand, within copAat 510.453 kb on + strandat 510.461 kb on - strandat 510.461 kb on - strandat 510.488 kb on + strandat 510.515 kb on - strandat 510.523 kb on - strandat 510.525 kb on + strandat 510.525 kb on + strandat 510.587 kb on - strandat 510.631 kb on - strandat 510.651 kb on - strandat 510.652 kb on - strandat 510.844 kb on + strandat 510.903 kb on + strandat 510.903 kb on + strandat 510.979 kb on - strand, within ybaSat 510.979 kb on - strand, within ybaSat 511.000 kb on - strand, within ybaSat 511.172 kb on + strand, within ybaSat 511.172 kb on + strand, within ybaSat 511.359 kb on + strand, within ybaSat 511.389 kb on - strand, within ybaSat 511.392 kb on - strand, within ybaSat 511.434 kb on + strand, within ybaSat 511.583 kb on + strand, within ybaSat 511.583 kb on + strand, within ybaSat 511.673 kb on + strand, within ybaSat 511.673 kb on + strand, within ybaSat 511.673 kb on + strand, within ybaSat 511.980 kb on + strand, within ybaTat 512.081 kb on + strand, within ybaTat 512.081 kb on + strand, within ybaTat 512.171 kb on + strand, within ybaTat 512.239 kb on - strand, within ybaTat 512.413 kb on - strand, within ybaTat 512.414 kb on - strand, within ybaTat 512.514 kb on - strand, within ybaTat 512.567 kb on - strand, within ybaTat 512.604 kb on + strand, within ybaTat 512.771 kb on + strand, within ybaT

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2
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509,929 + copA b0484 0.73 -1.6
509,935 + copA b0484 0.73 +1.8
510,004 - copA b0484 0.76 -2.2
510,019 + copA b0484 0.77 -3.1
510,186 + copA b0484 0.83 -0.9
510,217 - copA b0484 0.85 +0.9
510,217 - copA b0484 0.85 +0.3
510,264 - copA b0484 0.86 -1.3
510,266 + copA b0484 0.87 +0.2
510,272 + copA b0484 0.87 +1.9
510,277 + copA b0484 0.87 -0.9
510,453 + +0.3
510,461 - -0.7
510,461 - -0.2
510,488 + -3.1
510,515 - +1.7
510,523 - -2.3
510,525 + -2.9
510,525 + +1.6
510,587 - +0.8
510,631 - -0.9
510,651 - -3.1
510,652 - +0.3
510,844 + -0.2
510,903 + -2.9
510,903 + -0.6
510,979 - ybaS b0485 0.12 -0.9
510,979 - ybaS b0485 0.12 -1.3
511,000 - ybaS b0485 0.14 -2.1
511,172 + ybaS b0485 0.33 -3.1
511,172 + ybaS b0485 0.33 -0.3
511,359 + ybaS b0485 0.53 +0.5
511,389 - ybaS b0485 0.56 +0.3
511,392 - ybaS b0485 0.56 -0.0
511,434 + ybaS b0485 0.61 -2.5
511,583 + ybaS b0485 0.77 +0.3
511,583 + ybaS b0485 0.77 -3.2
511,673 + ybaS b0485 0.87 +0.0
511,673 + ybaS b0485 0.87 -0.3
511,673 + ybaS b0485 0.87 -0.1
511,980 + ybaT b0486 0.14 -1.0
512,081 + ybaT b0486 0.22 +0.9
512,081 + ybaT b0486 0.22 -2.7
512,171 + ybaT b0486 0.29 +0.4
512,239 - ybaT b0486 0.34 -1.5
512,413 - ybaT b0486 0.47 -0.4
512,414 - ybaT b0486 0.47 +0.3
512,514 - ybaT b0486 0.55 -0.4
512,567 - ybaT b0486 0.59 -3.9
512,604 + ybaT b0486 0.62 +0.7
512,771 + ybaT b0486 0.75 -1.1

Or see this region's nucleotide sequence