Strain Fitness in Escherichia coli BW25113 around b0472

Experiment: T7_phage 0.0015625 MOI TC2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaX and ybaB are separated by 52 nucleotidesybaB and recR overlap by 1 nucleotidesrecR and htpG are separated by 109 nucleotides b0470: dnaX - DNA polymerase III subunits gamma and tau (NCBI), at 491,316 to 493,247 dnaX b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629 ybaB b0472: recR - recombination protein RecR (NCBI), at 493,629 to 494,234 recR b0473: htpG - heat shock protein 90 (NCBI), at 494,344 to 496,218 htpG Position (kb) 493 494 495Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 493.296 kb on + strandat 493.296 kb on + strandat 493.320 kb on + strandat 493.327 kb on - strandat 493.392 kb on + strand, within ybaBat 493.488 kb on - strand, within ybaBat 493.499 kb on - strand, within ybaBat 493.499 kb on - strand, within ybaBat 493.517 kb on + strand, within ybaBat 493.713 kb on - strand, within recRat 493.724 kb on + strand, within recRat 493.879 kb on + strand, within recRat 493.882 kb on - strand, within recRat 493.887 kb on - strand, within recRat 493.887 kb on - strand, within recRat 493.949 kb on + strand, within recRat 493.993 kb on + strand, within recRat 494.016 kb on - strand, within recRat 494.016 kb on - strand, within recRat 494.016 kb on - strand, within recRat 494.018 kb on + strand, within recRat 494.057 kb on + strand, within recRat 494.074 kb on + strand, within recRat 494.094 kb on - strand, within recRat 494.099 kb on - strand, within recRat 494.115 kb on + strand, within recRat 494.211 kb on - strandat 494.211 kb on - strandat 494.211 kb on - strandat 494.291 kb on - strandat 494.345 kb on - strandat 494.345 kb on - strandat 494.448 kb on + strandat 494.517 kb on - strandat 494.561 kb on + strand, within htpGat 494.596 kb on - strand, within htpGat 494.619 kb on - strand, within htpGat 494.630 kb on - strand, within htpGat 494.658 kb on + strand, within htpGat 494.758 kb on + strand, within htpGat 494.821 kb on + strand, within htpGat 494.871 kb on - strand, within htpGat 494.871 kb on - strand, within htpGat 495.048 kb on - strand, within htpGat 495.108 kb on + strand, within htpGat 495.133 kb on + strand, within htpGat 495.155 kb on + strand, within htpGat 495.160 kb on + strand, within htpGat 495.164 kb on + strand, within htpGat 495.174 kb on + strand, within htpGat 495.205 kb on + strand, within htpG

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Per-strain Table

Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2
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493,296 + -2.1
493,296 + +3.5
493,320 + +1.2
493,327 - +3.7
493,392 + ybaB b0471 0.28 +0.3
493,488 - ybaB b0471 0.57 -0.9
493,499 - ybaB b0471 0.60 +1.8
493,499 - ybaB b0471 0.60 +2.1
493,517 + ybaB b0471 0.66 +2.3
493,713 - recR b0472 0.14 +2.9
493,724 + recR b0472 0.16 +2.3
493,879 + recR b0472 0.41 +2.0
493,882 - recR b0472 0.42 +2.2
493,887 - recR b0472 0.43 +2.0
493,887 - recR b0472 0.43 +3.0
493,949 + recR b0472 0.53 +2.9
493,993 + recR b0472 0.60 +3.1
494,016 - recR b0472 0.64 +3.3
494,016 - recR b0472 0.64 +1.1
494,016 - recR b0472 0.64 +2.6
494,018 + recR b0472 0.64 +3.0
494,057 + recR b0472 0.71 +2.8
494,074 + recR b0472 0.73 +3.1
494,094 - recR b0472 0.77 +2.1
494,099 - recR b0472 0.78 +2.7
494,115 + recR b0472 0.80 +2.5
494,211 - +1.7
494,211 - +4.9
494,211 - -2.0
494,291 - -2.6
494,345 - -2.0
494,345 - -1.1
494,448 + -0.7
494,517 - +1.3
494,561 + htpG b0473 0.12 -1.1
494,596 - htpG b0473 0.13 +0.3
494,619 - htpG b0473 0.15 +0.6
494,630 - htpG b0473 0.15 +0.3
494,658 + htpG b0473 0.17 +0.5
494,758 + htpG b0473 0.22 -1.3
494,821 + htpG b0473 0.25 +0.5
494,871 - htpG b0473 0.28 -0.8
494,871 - htpG b0473 0.28 -3.7
495,048 - htpG b0473 0.38 +1.1
495,108 + htpG b0473 0.41 -1.8
495,133 + htpG b0473 0.42 +0.5
495,155 + htpG b0473 0.43 -3.1
495,160 + htpG b0473 0.44 -2.8
495,164 + htpG b0473 0.44 -3.5
495,174 + htpG b0473 0.44 +0.9
495,205 + htpG b0473 0.46 +1.4

Or see this region's nucleotide sequence