Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagR and yagS overlap by 4 nucleotides yagS and yagT overlap by 4 nucleotides yagT and yagU are separated by 417 nucleotides
b0284: yagR - predicted oxidoreductase with molybdenum-binding domain (NCBI), at 297,960 to 300,158
yagR
b0285: yagS - predicted oxidoreductase with FAD-binding domain (NCBI), at 300,155 to 301,111
yagS
b0286: yagT - predicted xanthine dehydrogenase, 2Fe-2S subunit (NCBI), at 301,108 to 301,797
yagT
b0287: yagU - conserved inner membrane protein (NCBI), at 302,215 to 302,829
yagU
Position (kb) 301
302 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 300.109 kb on + strand at 300.109 kb on + strand at 300.176 kb on + strand at 300.181 kb on + strand at 300.181 kb on + strand at 300.196 kb on - strand at 300.268 kb on - strand, within yagS at 300.268 kb on - strand, within yagS at 300.325 kb on + strand, within yagS at 300.385 kb on + strand, within yagS at 300.483 kb on + strand, within yagS at 300.486 kb on - strand, within yagS at 300.486 kb on - strand, within yagS at 300.544 kb on + strand, within yagS at 300.552 kb on - strand, within yagS at 300.581 kb on + strand, within yagS at 300.719 kb on + strand, within yagS at 300.798 kb on + strand, within yagS at 300.829 kb on - strand, within yagS at 300.887 kb on - strand, within yagS at 300.916 kb on - strand, within yagS at 301.099 kb on - strand at 301.177 kb on + strand, within yagT at 301.287 kb on + strand, within yagT at 301.300 kb on + strand, within yagT at 301.300 kb on + strand, within yagT at 301.344 kb on - strand, within yagT at 301.428 kb on - strand, within yagT at 301.483 kb on - strand, within yagT at 301.483 kb on - strand, within yagT at 301.489 kb on - strand, within yagT at 301.580 kb on - strand, within yagT at 301.580 kb on - strand, within yagT at 301.638 kb on - strand, within yagT at 301.638 kb on - strand, within yagT at 301.672 kb on - strand, within yagT at 301.790 kb on - strand at 301.795 kb on + strand at 301.795 kb on + strand at 301.797 kb on + strand at 301.797 kb on + strand at 301.797 kb on + strand at 301.800 kb on - strand at 301.833 kb on - strand at 301.857 kb on - strand at 301.955 kb on + strand at 301.963 kb on - strand at 301.986 kb on + strand at 301.996 kb on - strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.031 kb on + strand at 302.033 kb on + strand at 302.033 kb on + strand at 302.033 kb on + strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.040 kb on + strand at 302.048 kb on - strand at 302.048 kb on - strand at 302.145 kb on + strand at 302.175 kb on + strand at 302.182 kb on + strand at 302.183 kb on - strand at 302.185 kb on + strand at 302.198 kb on - strand at 302.204 kb on - strand at 302.205 kb on - strand at 302.207 kb on + strand at 302.212 kb on + strand at 302.212 kb on + strand at 302.215 kb on - strand at 302.220 kb on - strand at 302.220 kb on - strand at 302.220 kb on - strand at 302.242 kb on + strand at 302.254 kb on - strand at 302.254 kb on - strand at 302.258 kb on + strand at 302.261 kb on - strand at 302.261 kb on - strand at 302.261 kb on - strand at 302.261 kb on - strand at 302.271 kb on + strand at 302.274 kb on - strand at 302.274 kb on - strand at 302.278 kb on - strand, within yagU at 302.293 kb on + strand, within yagU at 302.302 kb on + strand, within yagU at 302.304 kb on + strand, within yagU at 302.308 kb on + strand, within yagU at 302.316 kb on - strand, within yagU at 302.318 kb on - strand, within yagU at 302.340 kb on + strand, within yagU at 302.355 kb on + strand, within yagU at 302.355 kb on + strand, within yagU at 302.367 kb on + strand, within yagU at 302.377 kb on + strand, within yagU at 302.380 kb on + strand, within yagU at 302.380 kb on + strand, within yagU at 302.387 kb on - strand, within yagU at 302.403 kb on + strand, within yagU at 302.442 kb on - strand, within yagU at 302.457 kb on + strand, within yagU at 302.464 kb on - strand, within yagU at 302.470 kb on - strand, within yagU at 302.485 kb on + strand, within yagU at 302.485 kb on + strand, within yagU at 302.485 kb on + strand, within yagU at 302.495 kb on - strand, within yagU at 302.506 kb on + strand, within yagU at 302.508 kb on + strand, within yagU at 302.514 kb on - strand, within yagU at 302.514 kb on - strand, within yagU at 302.516 kb on + strand, within yagU at 302.516 kb on + strand, within yagU at 302.517 kb on + strand, within yagU at 302.517 kb on + strand, within yagU at 302.524 kb on + strand, within yagU at 302.524 kb on + strand, within yagU at 302.525 kb on - strand, within yagU at 302.530 kb on + strand, within yagU at 302.538 kb on - strand, within yagU at 302.561 kb on + strand, within yagU at 302.608 kb on + strand, within yagU at 302.608 kb on + strand, within yagU at 302.608 kb on + strand, within yagU at 302.616 kb on - strand, within yagU at 302.616 kb on - strand, within yagU at 302.623 kb on + strand, within yagU at 302.626 kb on - strand, within yagU at 302.656 kb on + strand, within yagU at 302.663 kb on + strand, within yagU at 302.664 kb on + strand, within yagU at 302.664 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.676 kb on - strand, within yagU at 302.690 kb on + strand, within yagU at 302.694 kb on + strand, within yagU at 302.780 kb on + strand at 302.780 kb on + strand at 302.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 300,109 + +1.2 300,109 + -2.8 300,176 + -0.5 300,181 + -3.4 300,181 + -1.3 300,196 - -0.5 300,268 - yagS b0285 0.12 +0.2 300,268 - yagS b0285 0.12 +0.2 300,325 + yagS b0285 0.18 +0.5 300,385 + yagS b0285 0.24 +1.9 300,483 + yagS b0285 0.34 -3.9 300,486 - yagS b0285 0.35 -2.2 300,486 - yagS b0285 0.35 -2.1 300,544 + yagS b0285 0.41 -0.1 300,552 - yagS b0285 0.41 +1.1 300,581 + yagS b0285 0.45 +0.9 300,719 + yagS b0285 0.59 -0.5 300,798 + yagS b0285 0.67 +1.0 300,829 - yagS b0285 0.70 -2.2 300,887 - yagS b0285 0.76 +1.2 300,916 - yagS b0285 0.80 +0.6 301,099 - +0.6 301,177 + yagT b0286 0.10 +2.2 301,287 + yagT b0286 0.26 -2.2 301,300 + yagT b0286 0.28 +0.9 301,300 + yagT b0286 0.28 -3.6 301,344 - yagT b0286 0.34 -4.2 301,428 - yagT b0286 0.46 -0.8 301,483 - yagT b0286 0.54 -0.9 301,483 - yagT b0286 0.54 +2.1 301,489 - yagT b0286 0.55 +1.9 301,580 - yagT b0286 0.68 -0.5 301,580 - yagT b0286 0.68 -1.2 301,638 - yagT b0286 0.77 +1.6 301,638 - yagT b0286 0.77 +3.5 301,672 - yagT b0286 0.82 +0.7 301,790 - -2.9 301,795 + +1.1 301,795 + -0.0 301,797 + -2.0 301,797 + -1.6 301,797 + +2.4 301,800 - -2.4 301,833 - +0.3 301,857 - +1.0 301,955 + -1.7 301,963 - +3.2 301,986 + -3.5 301,996 - -0.1 302,030 + +0.6 302,030 + -0.4 302,030 + +0.0 302,030 + +0.5 302,030 + +0.3 302,030 + +1.8 302,030 + +0.0 302,030 + +0.6 302,030 + +0.0 302,030 + +1.6 302,030 + +1.3 302,031 + +0.5 302,033 + -0.2 302,033 + -0.1 302,033 + +0.6 302,038 - +0.2 302,038 - +1.2 302,038 - -0.7 302,038 - +0.2 302,038 - -0.1 302,038 - -1.3 302,038 - +3.2 302,038 - +1.6 302,038 - -1.8 302,040 + -0.0 302,048 - -1.0 302,048 - -1.0 302,145 + -3.0 302,175 + -2.6 302,182 + +0.7 302,183 - +0.0 302,185 + -1.4 302,198 - +0.7 302,204 - -3.3 302,205 - +2.4 302,207 + -2.0 302,212 + -2.6 302,212 + -2.3 302,215 - -0.8 302,220 - +0.9 302,220 - +0.9 302,220 - +3.8 302,242 + +1.8 302,254 - +0.8 302,254 - -0.1 302,258 + -0.1 302,261 - -2.4 302,261 - -0.6 302,261 - -1.2 302,261 - +2.0 302,271 + +0.3 302,274 - +3.2 302,274 - +0.9 302,278 - yagU b0287 0.10 -1.4 302,293 + yagU b0287 0.13 -1.4 302,302 + yagU b0287 0.14 -1.3 302,304 + yagU b0287 0.14 -2.0 302,308 + yagU b0287 0.15 +2.2 302,316 - yagU b0287 0.16 -0.3 302,318 - yagU b0287 0.17 +1.2 302,340 + yagU b0287 0.20 -0.8 302,355 + yagU b0287 0.23 +1.5 302,355 + yagU b0287 0.23 -2.0 302,367 + yagU b0287 0.25 -1.1 302,377 + yagU b0287 0.26 -1.5 302,380 + yagU b0287 0.27 +0.4 302,380 + yagU b0287 0.27 -0.3 302,387 - yagU b0287 0.28 +1.2 302,403 + yagU b0287 0.31 +0.4 302,442 - yagU b0287 0.37 -0.4 302,457 + yagU b0287 0.39 -1.4 302,464 - yagU b0287 0.40 +1.6 302,470 - yagU b0287 0.41 +3.2 302,485 + yagU b0287 0.44 +0.9 302,485 + yagU b0287 0.44 -0.5 302,485 + yagU b0287 0.44 +0.6 302,495 - yagU b0287 0.46 +1.2 302,506 + yagU b0287 0.47 -0.1 302,508 + yagU b0287 0.48 -2.4 302,514 - yagU b0287 0.49 -0.4 302,514 - yagU b0287 0.49 +4.5 302,516 + yagU b0287 0.49 -2.4 302,516 + yagU b0287 0.49 -3.5 302,517 + yagU b0287 0.49 -1.4 302,517 + yagU b0287 0.49 -2.4 302,524 + yagU b0287 0.50 -0.0 302,524 + yagU b0287 0.50 -0.3 302,525 - yagU b0287 0.50 -0.5 302,530 + yagU b0287 0.51 -0.1 302,538 - yagU b0287 0.53 +1.6 302,561 + yagU b0287 0.56 -0.6 302,608 + yagU b0287 0.64 -0.9 302,608 + yagU b0287 0.64 +0.9 302,608 + yagU b0287 0.64 +0.0 302,616 - yagU b0287 0.65 +1.2 302,616 - yagU b0287 0.65 +3.7 302,623 + yagU b0287 0.66 -2.0 302,626 - yagU b0287 0.67 +0.9 302,656 + yagU b0287 0.72 +0.5 302,663 + yagU b0287 0.73 +1.4 302,664 + yagU b0287 0.73 +0.2 302,664 + yagU b0287 0.73 +1.3 302,668 + yagU b0287 0.74 -1.9 302,668 + yagU b0287 0.74 -0.2 302,668 + yagU b0287 0.74 -0.2 302,668 + yagU b0287 0.74 -0.0 302,675 + yagU b0287 0.75 +0.3 302,675 + yagU b0287 0.75 +0.2 302,675 + yagU b0287 0.75 +0.6 302,675 + yagU b0287 0.75 -0.7 302,675 + yagU b0287 0.75 -2.3 302,676 - yagU b0287 0.75 +3.2 302,690 + yagU b0287 0.77 +2.1 302,694 + yagU b0287 0.78 +0.0 302,780 + -2.1 302,780 + +0.9 302,782 + +0.4
Or see this region's nucleotide sequence