Experiment: T7_phage 0.0015625 MOI TC2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argF and ykgS are separated by 123 nucleotides ykgS and insB-3 are separated by 15 nucleotides insB-3 and insA-3 overlap by 82 nucleotides insA-3 and yagJ are separated by 57 nucleotides yagJ and yagK are separated by 90 nucleotides
b0273: argF - CP4-6 prophage; ornithine carbamoyltransferase 2, chain F (NCBI), at 288,525 to 289,529
argF
b4688: ykgS - no description, at 289,653 to 289,857
ykgS
b0274: insB-3 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 289,873 to 290,376
insB-3
b0275: insA-3 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 290,295 to 290,570
insA-3
b0276: yagJ - CP4-6 prophage; predicted protein (RefSeq), at 290,628 to 291,455
yagJ
b0277: yagK - CP4-6 prophage; conserved protein (NCBI), at 291,546 to 292,172
yagK
Position (kb) 290
291 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 289.480 kb on - strand at 289.483 kb on - strand at 289.636 kb on - strand at 289.687 kb on - strand, within ykgS at 290.734 kb on + strand, within yagJ at 290.749 kb on + strand, within yagJ at 290.762 kb on - strand, within yagJ at 290.767 kb on - strand, within yagJ at 290.767 kb on - strand, within yagJ at 290.824 kb on + strand, within yagJ at 290.832 kb on - strand, within yagJ at 290.916 kb on - strand, within yagJ at 290.943 kb on - strand, within yagJ at 290.997 kb on + strand, within yagJ at 291.015 kb on + strand, within yagJ at 291.015 kb on + strand, within yagJ at 291.025 kb on + strand, within yagJ at 291.041 kb on - strand, within yagJ at 291.110 kb on + strand, within yagJ at 291.115 kb on + strand, within yagJ at 291.118 kb on - strand, within yagJ at 291.118 kb on - strand, within yagJ at 291.118 kb on - strand, within yagJ at 291.159 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.161 kb on + strand, within yagJ at 291.169 kb on - strand at 291.169 kb on - strand, within yagJ at 291.178 kb on - strand, within yagJ at 291.249 kb on - strand, within yagJ at 291.249 kb on - strand, within yagJ at 291.268 kb on + strand, within yagJ at 291.344 kb on - strand, within yagJ at 291.374 kb on + strand at 291.435 kb on - strand at 291.435 kb on - strand at 291.446 kb on - strand at 291.446 kb on - strand at 291.449 kb on + strand at 291.514 kb on - strand at 291.545 kb on + strand at 291.545 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC2 remove 289,480 - +1.6 289,483 - +0.1 289,636 - +0.8 289,687 - ykgS b4688 0.17 -0.6 290,734 + yagJ b0276 0.13 +0.1 290,749 + yagJ b0276 0.15 -0.0 290,762 - yagJ b0276 0.16 -1.7 290,767 - yagJ b0276 0.17 -0.6 290,767 - yagJ b0276 0.17 -2.4 290,824 + yagJ b0276 0.24 +0.2 290,832 - yagJ b0276 0.25 +1.4 290,916 - yagJ b0276 0.35 +0.4 290,943 - yagJ b0276 0.38 -0.4 290,997 + yagJ b0276 0.45 -0.1 291,015 + yagJ b0276 0.47 -2.7 291,015 + yagJ b0276 0.47 -0.9 291,025 + yagJ b0276 0.48 -2.7 291,041 - yagJ b0276 0.50 +0.5 291,110 + yagJ b0276 0.58 +0.3 291,115 + yagJ b0276 0.59 -1.6 291,118 - yagJ b0276 0.59 +1.2 291,118 - yagJ b0276 0.59 +3.3 291,118 - yagJ b0276 0.59 +0.7 291,159 + yagJ b0276 0.64 +1.0 291,161 + yagJ b0276 0.64 +0.8 291,161 + yagJ b0276 0.64 +1.3 291,161 + yagJ b0276 0.64 -0.6 291,161 + yagJ b0276 0.64 +1.4 291,161 + yagJ b0276 0.64 -1.4 291,169 - +1.6 291,169 - yagJ b0276 0.65 -1.2 291,178 - yagJ b0276 0.66 -2.9 291,249 - yagJ b0276 0.75 -0.9 291,249 - yagJ b0276 0.75 -1.1 291,268 + yagJ b0276 0.77 +0.3 291,344 - yagJ b0276 0.86 -0.1 291,374 + +0.8 291,435 - -1.5 291,435 - -1.4 291,446 - -0.3 291,446 - +0.5 291,449 + +1.3 291,514 - +1.4 291,545 + +1.1 291,545 + +1.4
Or see this region's nucleotide sequence