Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfN and ytfP are separated by 2 nucleotides ytfP and chpS are separated by 211 nucleotides chpS and chpB overlap by 7 nucleotides chpB and ppa are separated by 79 nucleotides
b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914
ytfN
b4222: ytfP - hypothetical protein (NCBI), at 4,445,917 to 4,446,258
ytfP
b4224: chpS - suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS), at 4,446,470 to 4,446,721
chpS
b4225: chpB - toxin of the ChpB-ChpS toxin-antitoxin system (NCBI), at 4,446,715 to 4,447,065
chpB
b4226: ppa - inorganic pyrophosphatase (NCBI), at 4,447,145 to 4,447,675
ppa
Position (kb)
4446
4447 Strain fitness (log2 ratio)
-1
0
1 at 4445.535 kb on - strand, within ytfN at 4445.639 kb on + strand at 4445.668 kb on - strand at 4445.668 kb on - strand at 4445.736 kb on + strand at 4445.737 kb on + strand at 4445.737 kb on + strand at 4445.770 kb on - strand at 4445.794 kb on + strand at 4445.806 kb on + strand at 4445.806 kb on + strand at 4445.806 kb on + strand at 4445.836 kb on + strand at 4445.836 kb on + strand at 4445.859 kb on - strand at 4445.866 kb on + strand at 4445.866 kb on + strand at 4445.962 kb on + strand, within ytfP at 4445.962 kb on + strand, within ytfP at 4446.046 kb on - strand, within ytfP at 4446.131 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.176 kb on + strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.184 kb on - strand, within ytfP at 4446.184 kb on - strand, within ytfP at 4446.240 kb on + strand at 4446.259 kb on + strand at 4446.260 kb on + strand at 4446.272 kb on - strand at 4446.273 kb on - strand at 4446.273 kb on - strand at 4446.375 kb on - strand at 4446.454 kb on + strand at 4446.454 kb on + strand at 4446.496 kb on + strand, within chpS at 4446.501 kb on + strand, within chpS at 4446.501 kb on + strand, within chpS at 4446.509 kb on - strand, within chpS at 4446.509 kb on - strand, within chpS at 4446.514 kb on - strand, within chpS at 4446.559 kb on + strand, within chpS at 4446.569 kb on - strand, within chpS at 4446.569 kb on - strand, within chpS at 4446.646 kb on - strand, within chpS at 4446.652 kb on - strand, within chpS at 4446.746 kb on + strand at 4446.777 kb on + strand, within chpB at 4446.787 kb on + strand, within chpB at 4446.787 kb on + strand, within chpB at 4446.787 kb on - strand, within chpB at 4446.787 kb on - strand, within chpB at 4446.794 kb on + strand, within chpB at 4446.910 kb on + strand, within chpB at 4446.916 kb on - strand, within chpB at 4446.945 kb on + strand, within chpB at 4446.949 kb on - strand, within chpB at 4446.960 kb on + strand, within chpB at 4447.006 kb on - strand, within chpB at 4447.006 kb on - strand, within chpB at 4447.057 kb on + strand at 4447.060 kb on + strand at 4447.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 4,445,535 - ytfN b4221 0.90 -1.4 4,445,639 + -0.1 4,445,668 - +0.3 4,445,668 - +1.3 4,445,736 + +0.2 4,445,737 + -1.5 4,445,737 + +1.2 4,445,770 - +1.3 4,445,794 + -0.2 4,445,806 + +0.4 4,445,806 + -0.3 4,445,806 + +0.3 4,445,836 + +0.1 4,445,836 + +0.1 4,445,859 - +0.8 4,445,866 + -1.4 4,445,866 + +0.2 4,445,962 + ytfP b4222 0.13 +0.0 4,445,962 + ytfP b4222 0.13 -1.3 4,446,046 - ytfP b4222 0.38 -0.3 4,446,131 + ytfP b4222 0.63 -0.4 4,446,166 + ytfP b4222 0.73 +0.5 4,446,166 + ytfP b4222 0.73 -0.6 4,446,166 + ytfP b4222 0.73 +1.5 4,446,176 + ytfP b4222 0.76 +0.2 4,446,176 - ytfP b4222 0.76 -1.3 4,446,176 - ytfP b4222 0.76 +0.4 4,446,176 - ytfP b4222 0.76 -0.5 4,446,184 - ytfP b4222 0.78 +0.9 4,446,184 - ytfP b4222 0.78 +0.1 4,446,240 + +0.6 4,446,259 + -0.2 4,446,260 + -0.0 4,446,272 - -0.2 4,446,273 - -1.1 4,446,273 - -0.3 4,446,375 - +0.7 4,446,454 + -0.8 4,446,454 + +0.3 4,446,496 + chpS b4224 0.10 +0.4 4,446,501 + chpS b4224 0.12 -0.1 4,446,501 + chpS b4224 0.12 +0.3 4,446,509 - chpS b4224 0.15 +0.7 4,446,509 - chpS b4224 0.15 +0.5 4,446,514 - chpS b4224 0.17 +0.7 4,446,559 + chpS b4224 0.35 +1.3 4,446,569 - chpS b4224 0.39 +0.6 4,446,569 - chpS b4224 0.39 +0.4 4,446,646 - chpS b4224 0.70 +0.1 4,446,652 - chpS b4224 0.72 -0.4 4,446,746 + +0.5 4,446,777 + chpB b4225 0.18 -0.1 4,446,787 + chpB b4225 0.21 +0.3 4,446,787 + chpB b4225 0.21 -0.1 4,446,787 - chpB b4225 0.21 +0.2 4,446,787 - chpB b4225 0.21 +0.3 4,446,794 + chpB b4225 0.23 +0.8 4,446,910 + chpB b4225 0.56 +0.4 4,446,916 - chpB b4225 0.57 +0.6 4,446,945 + chpB b4225 0.66 +0.4 4,446,949 - chpB b4225 0.67 -0.2 4,446,960 + chpB b4225 0.70 -0.3 4,447,006 - chpB b4225 0.83 +0.5 4,447,006 - chpB b4225 0.83 -0.1 4,447,057 + +0.4 4,447,060 + -0.5 4,447,060 + -0.3
Or see this region's nucleotide sequence