Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfA and ytfB overlap by 17 nucleotides ytfB and fklB are separated by 217 nucleotides fklB and cycA are separated by 308 nucleotides
b4205: ytfA - predicted transcriptional regulator (RefSeq), at 4,425,717 to 4,426,118
ytfA
b4206: ytfB - orf, hypothetical protein (VIMSS), at 4,426,102 to 4,426,740
ytfB
b4207: fklB - FKBP-type 22KD peptidyl-prolyl cis-trans isomerase (rotamase) (VIMSS), at 4,426,958 to 4,427,578
fklB
b4208: cycA - D-alanine/D-serine/glycine transporter (NCBI), at 4,427,887 to 4,429,299
cycA
Position (kb)
4426
4427
4428 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4425.996 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.997 kb on - strand, within ytfA at 4425.999 kb on + strand, within ytfA at 4425.999 kb on + strand, within ytfA at 4426.009 kb on - strand, within ytfA at 4426.013 kb on - strand, within ytfA at 4426.016 kb on - strand, within ytfA at 4426.016 kb on - strand, within ytfA at 4426.021 kb on - strand, within ytfA at 4426.021 kb on - strand, within ytfA at 4426.021 kb on - strand, within ytfA at 4426.031 kb on - strand, within ytfA at 4426.031 kb on - strand, within ytfA at 4426.031 kb on - strand, within ytfA at 4426.031 kb on - strand, within ytfA at 4426.031 kb on - strand, within ytfA at 4426.035 kb on + strand, within ytfA at 4426.035 kb on + strand, within ytfA at 4426.035 kb on + strand, within ytfA at 4426.042 kb on - strand, within ytfA at 4426.043 kb on - strand, within ytfA at 4426.043 kb on - strand, within ytfA at 4426.046 kb on - strand, within ytfA at 4426.046 kb on - strand, within ytfA at 4426.046 kb on - strand, within ytfA at 4426.052 kb on - strand, within ytfA at 4426.053 kb on - strand, within ytfA at 4426.053 kb on - strand, within ytfA at 4426.053 kb on - strand, within ytfA at 4426.055 kb on - strand, within ytfA at 4426.056 kb on - strand, within ytfA at 4426.057 kb on - strand, within ytfA at 4426.069 kb on - strand, within ytfA at 4426.090 kb on - strand at 4426.090 kb on - strand at 4426.097 kb on + strand at 4426.099 kb on + strand at 4426.099 kb on + strand at 4426.102 kb on - strand at 4426.105 kb on - strand at 4426.105 kb on - strand at 4426.105 kb on - strand at 4426.124 kb on - strand at 4426.169 kb on + strand, within ytfB at 4426.169 kb on + strand, within ytfB at 4426.172 kb on + strand, within ytfB at 4426.182 kb on - strand, within ytfB at 4426.188 kb on - strand, within ytfB at 4426.191 kb on - strand, within ytfB at 4426.191 kb on - strand, within ytfB at 4426.201 kb on - strand, within ytfB at 4426.248 kb on + strand, within ytfB at 4426.248 kb on + strand, within ytfB at 4426.259 kb on + strand, within ytfB at 4426.259 kb on + strand, within ytfB at 4426.267 kb on - strand, within ytfB at 4426.277 kb on + strand, within ytfB at 4426.280 kb on - strand, within ytfB at 4426.292 kb on - strand, within ytfB at 4426.307 kb on - strand, within ytfB at 4426.443 kb on + strand, within ytfB at 4426.451 kb on - strand, within ytfB at 4426.577 kb on - strand, within ytfB at 4426.673 kb on - strand, within ytfB at 4426.673 kb on - strand, within ytfB at 4426.673 kb on - strand, within ytfB at 4426.731 kb on + strand at 4426.731 kb on + strand at 4426.747 kb on + strand at 4426.750 kb on - strand at 4426.755 kb on - strand at 4426.789 kb on + strand at 4426.792 kb on - strand at 4426.792 kb on - strand at 4426.844 kb on + strand at 4426.844 kb on + strand at 4426.878 kb on - strand at 4426.911 kb on + strand at 4426.911 kb on + strand at 4426.912 kb on + strand at 4426.922 kb on - strand at 4426.922 kb on - strand at 4426.932 kb on + strand at 4426.932 kb on + strand at 4426.933 kb on + strand at 4426.965 kb on - strand at 4427.007 kb on - strand at 4427.007 kb on - strand at 4427.009 kb on + strand at 4427.022 kb on + strand, within fklB at 4427.114 kb on + strand, within fklB at 4427.132 kb on - strand, within fklB at 4427.161 kb on + strand, within fklB at 4427.215 kb on - strand, within fklB at 4427.243 kb on + strand, within fklB at 4427.251 kb on - strand, within fklB at 4427.251 kb on - strand, within fklB at 4427.251 kb on - strand, within fklB at 4427.298 kb on - strand at 4427.298 kb on - strand, within fklB at 4427.345 kb on + strand, within fklB at 4427.371 kb on - strand, within fklB at 4427.371 kb on - strand, within fklB at 4427.371 kb on - strand, within fklB at 4427.381 kb on - strand, within fklB at 4427.394 kb on - strand, within fklB at 4427.394 kb on - strand, within fklB at 4427.415 kb on + strand, within fklB at 4427.415 kb on + strand, within fklB at 4427.541 kb on - strand at 4427.541 kb on - strand at 4427.563 kb on + strand at 4427.563 kb on + strand at 4427.580 kb on - strand at 4427.624 kb on - strand at 4427.710 kb on + strand at 4427.783 kb on + strand at 4427.783 kb on + strand at 4427.788 kb on + strand at 4427.806 kb on - strand at 4427.830 kb on - strand at 4427.830 kb on - strand at 4427.840 kb on + strand at 4427.840 kb on + strand at 4427.840 kb on + strand at 4427.850 kb on + strand at 4427.852 kb on - strand at 4427.852 kb on - strand at 4427.906 kb on - strand at 4427.906 kb on - strand at 4427.906 kb on - strand at 4427.961 kb on + strand at 4427.961 kb on + strand at 4427.990 kb on + strand at 4428.012 kb on - strand at 4428.038 kb on + strand, within cycA at 4428.110 kb on + strand, within cycA at 4428.171 kb on - strand, within cycA at 4428.256 kb on + strand, within cycA at 4428.256 kb on + strand, within cycA at 4428.257 kb on + strand, within cycA at 4428.265 kb on - strand, within cycA at 4428.270 kb on - strand, within cycA at 4428.275 kb on - strand, within cycA at 4428.283 kb on - strand, within cycA at 4428.293 kb on + strand, within cycA at 4428.293 kb on + strand, within cycA at 4428.314 kb on - strand, within cycA at 4428.337 kb on - strand, within cycA at 4428.375 kb on - strand, within cycA at 4428.398 kb on + strand, within cycA at 4428.422 kb on + strand, within cycA at 4428.427 kb on + strand, within cycA at 4428.454 kb on - strand, within cycA
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 4,425,996 - ytfA b4205 0.69 -0.2 4,425,997 - ytfA b4205 0.70 -1.1 4,425,997 - ytfA b4205 0.70 -1.2 4,425,997 - ytfA b4205 0.70 -0.7 4,425,997 - ytfA b4205 0.70 -1.0 4,425,997 - ytfA b4205 0.70 +0.2 4,425,997 - ytfA b4205 0.70 -0.2 4,425,997 - ytfA b4205 0.70 -0.0 4,425,997 - ytfA b4205 0.70 -0.9 4,425,997 - ytfA b4205 0.70 -0.4 4,425,999 + ytfA b4205 0.70 +0.6 4,425,999 + ytfA b4205 0.70 +2.6 4,426,009 - ytfA b4205 0.73 -1.0 4,426,013 - ytfA b4205 0.74 -0.4 4,426,016 - ytfA b4205 0.74 -0.3 4,426,016 - ytfA b4205 0.74 -1.9 4,426,021 - ytfA b4205 0.76 +0.4 4,426,021 - ytfA b4205 0.76 -0.5 4,426,021 - ytfA b4205 0.76 -0.2 4,426,031 - ytfA b4205 0.78 -0.6 4,426,031 - ytfA b4205 0.78 +1.0 4,426,031 - ytfA b4205 0.78 +0.3 4,426,031 - ytfA b4205 0.78 -0.1 4,426,031 - ytfA b4205 0.78 +0.4 4,426,035 + ytfA b4205 0.79 +2.6 4,426,035 + ytfA b4205 0.79 +2.6 4,426,035 + ytfA b4205 0.79 +0.1 4,426,042 - ytfA b4205 0.81 -0.4 4,426,043 - ytfA b4205 0.81 -0.7 4,426,043 - ytfA b4205 0.81 +0.1 4,426,046 - ytfA b4205 0.82 +0.3 4,426,046 - ytfA b4205 0.82 +0.5 4,426,046 - ytfA b4205 0.82 +0.6 4,426,052 - ytfA b4205 0.83 -0.0 4,426,053 - ytfA b4205 0.84 +1.0 4,426,053 - ytfA b4205 0.84 +0.7 4,426,053 - ytfA b4205 0.84 -0.3 4,426,055 - ytfA b4205 0.84 +1.1 4,426,056 - ytfA b4205 0.84 -0.3 4,426,057 - ytfA b4205 0.85 -0.1 4,426,069 - ytfA b4205 0.88 +0.2 4,426,090 - +0.0 4,426,090 - -0.3 4,426,097 + +0.6 4,426,099 + +0.7 4,426,099 + +0.1 4,426,102 - +0.5 4,426,105 - +1.7 4,426,105 - +1.1 4,426,105 - -0.0 4,426,124 - +0.0 4,426,169 + ytfB b4206 0.10 -0.7 4,426,169 + ytfB b4206 0.10 -0.2 4,426,172 + ytfB b4206 0.11 +0.1 4,426,182 - ytfB b4206 0.13 -0.7 4,426,188 - ytfB b4206 0.13 +1.2 4,426,191 - ytfB b4206 0.14 -1.0 4,426,191 - ytfB b4206 0.14 +0.3 4,426,201 - ytfB b4206 0.15 -0.3 4,426,248 + ytfB b4206 0.23 -2.2 4,426,248 + ytfB b4206 0.23 +0.3 4,426,259 + ytfB b4206 0.25 +0.6 4,426,259 + ytfB b4206 0.25 -0.3 4,426,267 - ytfB b4206 0.26 +0.5 4,426,277 + ytfB b4206 0.27 -0.7 4,426,280 - ytfB b4206 0.28 -2.2 4,426,292 - ytfB b4206 0.30 -0.6 4,426,307 - ytfB b4206 0.32 -0.0 4,426,443 + ytfB b4206 0.53 -0.3 4,426,451 - ytfB b4206 0.55 +0.3 4,426,577 - ytfB b4206 0.74 +0.2 4,426,673 - ytfB b4206 0.89 +0.0 4,426,673 - ytfB b4206 0.89 -0.5 4,426,673 - ytfB b4206 0.89 +0.6 4,426,731 + +0.6 4,426,731 + +0.4 4,426,747 + +0.8 4,426,750 - -0.1 4,426,755 - -0.1 4,426,789 + -0.4 4,426,792 - -0.3 4,426,792 - -0.6 4,426,844 + +0.3 4,426,844 + +0.1 4,426,878 - -0.8 4,426,911 + -0.3 4,426,911 + -1.2 4,426,912 + -0.5 4,426,922 - +0.5 4,426,922 - -0.4 4,426,932 + +0.3 4,426,932 + +1.7 4,426,933 + -0.7 4,426,965 - -0.9 4,427,007 - -0.9 4,427,007 - -0.3 4,427,009 + -0.6 4,427,022 + fklB b4207 0.10 -0.4 4,427,114 + fklB b4207 0.25 +0.3 4,427,132 - fklB b4207 0.28 +0.3 4,427,161 + fklB b4207 0.33 +0.0 4,427,215 - fklB b4207 0.41 +1.0 4,427,243 + fklB b4207 0.46 -0.0 4,427,251 - fklB b4207 0.47 +0.7 4,427,251 - fklB b4207 0.47 +0.5 4,427,251 - fklB b4207 0.47 +0.0 4,427,298 - -0.4 4,427,298 - fklB b4207 0.55 +0.9 4,427,345 + fklB b4207 0.62 -0.0 4,427,371 - fklB b4207 0.67 -1.8 4,427,371 - fklB b4207 0.67 +0.1 4,427,371 - fklB b4207 0.67 +0.4 4,427,381 - fklB b4207 0.68 -0.2 4,427,394 - fklB b4207 0.70 +0.8 4,427,394 - fklB b4207 0.70 +0.1 4,427,415 + fklB b4207 0.74 +1.2 4,427,415 + fklB b4207 0.74 +0.7 4,427,541 - +0.5 4,427,541 - +0.9 4,427,563 + +0.5 4,427,563 + +0.3 4,427,580 - -0.5 4,427,624 - -0.4 4,427,710 + -0.3 4,427,783 + -0.5 4,427,783 + -0.2 4,427,788 + -0.3 4,427,806 - -0.1 4,427,830 - +0.5 4,427,830 - -1.4 4,427,840 + +0.2 4,427,840 + +0.1 4,427,840 + -1.0 4,427,850 + -0.8 4,427,852 - +0.4 4,427,852 - -0.1 4,427,906 - +0.8 4,427,906 - +0.2 4,427,906 - -0.3 4,427,961 + +0.2 4,427,961 + -0.9 4,427,990 + -0.6 4,428,012 - +0.2 4,428,038 + cycA b4208 0.11 -0.3 4,428,110 + cycA b4208 0.16 +0.5 4,428,171 - cycA b4208 0.20 -0.2 4,428,256 + cycA b4208 0.26 -1.3 4,428,256 + cycA b4208 0.26 -0.6 4,428,257 + cycA b4208 0.26 -0.3 4,428,265 - cycA b4208 0.27 -0.4 4,428,270 - cycA b4208 0.27 -3.3 4,428,275 - cycA b4208 0.27 +0.5 4,428,283 - cycA b4208 0.28 -0.1 4,428,293 + cycA b4208 0.29 -0.1 4,428,293 + cycA b4208 0.29 -0.0 4,428,314 - cycA b4208 0.30 +1.0 4,428,337 - cycA b4208 0.32 +0.1 4,428,375 - cycA b4208 0.35 +0.0 4,428,398 + cycA b4208 0.36 +1.0 4,428,422 + cycA b4208 0.38 +0.5 4,428,427 + cycA b4208 0.38 +0.8 4,428,454 - cycA b4208 0.40 -1.0
Or see this region's nucleotide sequence