Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fieF and pfkA are separated by 180 nucleotides pfkA and sbp are separated by 319 nucleotides
b3915: fieF - ferrous iron efflux protein F (NCBI), at 4,104,492 to 4,105,394
fieF
b3916: pfkA - 6-phosphofructokinase (NCBI), at 4,105,575 to 4,106,537
pfkA
b3917: sbp - sulfate transporter subunit (NCBI), at 4,106,857 to 4,107,846
sbp
Position (kb)
4105
4106
4107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4104.584 kb on + strand, within fieF at 4104.584 kb on + strand, within fieF at 4104.592 kb on - strand, within fieF at 4104.592 kb on - strand, within fieF at 4104.592 kb on - strand, within fieF at 4104.592 kb on - strand, within fieF at 4104.615 kb on + strand, within fieF at 4104.615 kb on + strand, within fieF at 4104.625 kb on + strand, within fieF at 4104.682 kb on - strand, within fieF at 4104.698 kb on - strand, within fieF at 4104.698 kb on - strand, within fieF at 4104.724 kb on - strand, within fieF at 4104.726 kb on - strand, within fieF at 4104.726 kb on - strand, within fieF at 4104.751 kb on + strand, within fieF at 4104.766 kb on + strand, within fieF at 4104.771 kb on + strand, within fieF at 4104.785 kb on + strand, within fieF at 4104.841 kb on + strand, within fieF at 4104.849 kb on - strand, within fieF at 4104.849 kb on - strand, within fieF at 4104.896 kb on + strand, within fieF at 4104.896 kb on + strand, within fieF at 4104.920 kb on + strand, within fieF at 4104.945 kb on + strand, within fieF at 4104.950 kb on - strand, within fieF at 4104.956 kb on + strand, within fieF at 4104.956 kb on + strand, within fieF at 4105.001 kb on + strand, within fieF at 4105.001 kb on + strand, within fieF at 4105.001 kb on + strand, within fieF at 4105.004 kb on - strand, within fieF at 4105.005 kb on + strand, within fieF at 4105.009 kb on - strand, within fieF at 4105.019 kb on - strand, within fieF at 4105.019 kb on - strand, within fieF at 4105.024 kb on + strand, within fieF at 4105.084 kb on - strand, within fieF at 4105.112 kb on - strand, within fieF at 4105.115 kb on + strand, within fieF at 4105.123 kb on - strand, within fieF at 4105.123 kb on - strand, within fieF at 4105.204 kb on + strand, within fieF at 4105.204 kb on + strand, within fieF at 4105.204 kb on + strand, within fieF at 4105.204 kb on + strand, within fieF at 4105.279 kb on - strand, within fieF at 4105.288 kb on + strand, within fieF at 4105.297 kb on + strand, within fieF at 4105.301 kb on - strand, within fieF at 4105.346 kb on + strand at 4105.346 kb on + strand at 4105.356 kb on + strand at 4105.361 kb on + strand at 4105.388 kb on + strand at 4105.398 kb on + strand at 4105.398 kb on + strand at 4105.452 kb on + strand at 4105.453 kb on + strand at 4105.517 kb on - strand at 4105.569 kb on + strand at 4105.569 kb on + strand at 4105.569 kb on - strand at 4105.571 kb on + strand at 4105.620 kb on + strand at 4105.620 kb on + strand at 4105.718 kb on - strand, within pfkA at 4105.718 kb on - strand, within pfkA at 4105.728 kb on + strand, within pfkA at 4105.728 kb on - strand, within pfkA at 4105.746 kb on - strand, within pfkA at 4105.752 kb on + strand, within pfkA at 4105.768 kb on - strand, within pfkA at 4105.890 kb on + strand, within pfkA at 4106.003 kb on + strand, within pfkA at 4106.004 kb on - strand, within pfkA at 4106.014 kb on + strand, within pfkA at 4106.014 kb on + strand, within pfkA at 4106.022 kb on - strand, within pfkA at 4106.044 kb on + strand, within pfkA at 4106.070 kb on - strand, within pfkA at 4106.324 kb on + strand, within pfkA at 4106.325 kb on + strand, within pfkA at 4106.417 kb on - strand, within pfkA at 4106.425 kb on + strand, within pfkA at 4106.433 kb on - strand, within pfkA at 4106.465 kb on + strand at 4106.482 kb on + strand at 4106.482 kb on + strand at 4106.495 kb on + strand at 4106.495 kb on + strand at 4106.508 kb on + strand at 4106.508 kb on + strand at 4106.516 kb on - strand at 4106.616 kb on - strand at 4106.717 kb on + strand at 4106.717 kb on + strand at 4106.760 kb on - strand at 4106.824 kb on + strand at 4106.827 kb on + strand at 4106.839 kb on + strand at 4106.839 kb on + strand at 4106.839 kb on + strand at 4106.847 kb on - strand at 4106.917 kb on + strand at 4106.923 kb on - strand at 4106.923 kb on - strand at 4106.970 kb on + strand, within sbp at 4106.970 kb on + strand, within sbp at 4106.970 kb on + strand, within sbp at 4106.999 kb on - strand, within sbp at 4106.999 kb on - strand, within sbp at 4107.116 kb on - strand, within sbp at 4107.144 kb on + strand, within sbp at 4107.159 kb on + strand, within sbp at 4107.167 kb on + strand, within sbp at 4107.179 kb on + strand, within sbp at 4107.187 kb on - strand, within sbp at 4107.189 kb on + strand, within sbp at 4107.238 kb on + strand, within sbp at 4107.292 kb on - strand, within sbp at 4107.292 kb on - strand, within sbp at 4107.299 kb on + strand, within sbp at 4107.321 kb on - strand, within sbp at 4107.321 kb on - strand, within sbp at 4107.329 kb on - strand, within sbp at 4107.339 kb on - strand, within sbp at 4107.357 kb on + strand, within sbp at 4107.357 kb on + strand, within sbp at 4107.369 kb on + strand, within sbp at 4107.369 kb on + strand, within sbp at 4107.472 kb on - strand, within sbp at 4107.502 kb on + strand, within sbp
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 4,104,584 + fieF b3915 0.10 +0.8 4,104,584 + fieF b3915 0.10 +0.2 4,104,592 - fieF b3915 0.11 -0.2 4,104,592 - fieF b3915 0.11 +0.1 4,104,592 - fieF b3915 0.11 +0.2 4,104,592 - fieF b3915 0.11 +0.1 4,104,615 + fieF b3915 0.14 -0.3 4,104,615 + fieF b3915 0.14 -0.9 4,104,625 + fieF b3915 0.15 +0.4 4,104,682 - fieF b3915 0.21 +0.7 4,104,698 - fieF b3915 0.23 +1.1 4,104,698 - fieF b3915 0.23 +1.2 4,104,724 - fieF b3915 0.26 +0.0 4,104,726 - fieF b3915 0.26 -0.4 4,104,726 - fieF b3915 0.26 -0.1 4,104,751 + fieF b3915 0.29 +0.2 4,104,766 + fieF b3915 0.30 -0.3 4,104,771 + fieF b3915 0.31 +0.5 4,104,785 + fieF b3915 0.32 +0.2 4,104,841 + fieF b3915 0.39 -1.1 4,104,849 - fieF b3915 0.40 -0.4 4,104,849 - fieF b3915 0.40 -0.5 4,104,896 + fieF b3915 0.45 +0.3 4,104,896 + fieF b3915 0.45 -0.9 4,104,920 + fieF b3915 0.47 -0.6 4,104,945 + fieF b3915 0.50 -0.2 4,104,950 - fieF b3915 0.51 +0.1 4,104,956 + fieF b3915 0.51 -1.6 4,104,956 + fieF b3915 0.51 -0.7 4,105,001 + fieF b3915 0.56 -0.1 4,105,001 + fieF b3915 0.56 -0.9 4,105,001 + fieF b3915 0.56 -0.5 4,105,004 - fieF b3915 0.57 -0.7 4,105,005 + fieF b3915 0.57 -0.3 4,105,009 - fieF b3915 0.57 -0.2 4,105,019 - fieF b3915 0.58 +0.6 4,105,019 - fieF b3915 0.58 -0.4 4,105,024 + fieF b3915 0.59 -0.4 4,105,084 - fieF b3915 0.66 -2.5 4,105,112 - fieF b3915 0.69 -0.5 4,105,115 + fieF b3915 0.69 -1.4 4,105,123 - fieF b3915 0.70 +0.3 4,105,123 - fieF b3915 0.70 -0.2 4,105,204 + fieF b3915 0.79 -0.3 4,105,204 + fieF b3915 0.79 -0.0 4,105,204 + fieF b3915 0.79 -0.8 4,105,204 + fieF b3915 0.79 +0.4 4,105,279 - fieF b3915 0.87 +0.1 4,105,288 + fieF b3915 0.88 +0.4 4,105,297 + fieF b3915 0.89 -0.1 4,105,301 - fieF b3915 0.90 +0.8 4,105,346 + -0.4 4,105,346 + +0.5 4,105,356 + -0.2 4,105,361 + -0.2 4,105,388 + -0.4 4,105,398 + +0.0 4,105,398 + -0.6 4,105,452 + -0.3 4,105,453 + +1.2 4,105,517 - +0.8 4,105,569 + +0.0 4,105,569 + -0.0 4,105,569 - -0.4 4,105,571 + +0.5 4,105,620 + +0.8 4,105,620 + +0.5 4,105,718 - pfkA b3916 0.15 +0.8 4,105,718 - pfkA b3916 0.15 -0.9 4,105,728 + pfkA b3916 0.16 -0.7 4,105,728 - pfkA b3916 0.16 +3.0 4,105,746 - pfkA b3916 0.18 +0.2 4,105,752 + pfkA b3916 0.18 +0.0 4,105,768 - pfkA b3916 0.20 +1.8 4,105,890 + pfkA b3916 0.33 -1.4 4,106,003 + pfkA b3916 0.44 +0.9 4,106,004 - pfkA b3916 0.45 -0.5 4,106,014 + pfkA b3916 0.46 -1.1 4,106,014 + pfkA b3916 0.46 -0.4 4,106,022 - pfkA b3916 0.46 +0.0 4,106,044 + pfkA b3916 0.49 -0.5 4,106,070 - pfkA b3916 0.51 -1.6 4,106,324 + pfkA b3916 0.78 +0.5 4,106,325 + pfkA b3916 0.78 -0.4 4,106,417 - pfkA b3916 0.87 +0.8 4,106,425 + pfkA b3916 0.88 +0.6 4,106,433 - pfkA b3916 0.89 +0.8 4,106,465 + +0.5 4,106,482 + -0.5 4,106,482 + -0.8 4,106,495 + +0.7 4,106,495 + +0.7 4,106,508 + +2.0 4,106,508 + -0.5 4,106,516 - -0.7 4,106,616 - -0.7 4,106,717 + +0.7 4,106,717 + -0.5 4,106,760 - -0.3 4,106,824 + -0.4 4,106,827 + -0.1 4,106,839 + -0.3 4,106,839 + -1.9 4,106,839 + +0.3 4,106,847 - +0.5 4,106,917 + -0.9 4,106,923 - -0.1 4,106,923 - -0.6 4,106,970 + sbp b3917 0.11 +0.1 4,106,970 + sbp b3917 0.11 +0.2 4,106,970 + sbp b3917 0.11 -0.2 4,106,999 - sbp b3917 0.14 -1.1 4,106,999 - sbp b3917 0.14 +0.1 4,107,116 - sbp b3917 0.26 -0.8 4,107,144 + sbp b3917 0.29 +0.7 4,107,159 + sbp b3917 0.31 -0.8 4,107,167 + sbp b3917 0.31 -0.1 4,107,179 + sbp b3917 0.33 -0.4 4,107,187 - sbp b3917 0.33 -0.6 4,107,189 + sbp b3917 0.34 -0.2 4,107,238 + sbp b3917 0.38 +0.0 4,107,292 - sbp b3917 0.44 +0.3 4,107,292 - sbp b3917 0.44 -0.2 4,107,299 + sbp b3917 0.45 +1.3 4,107,321 - sbp b3917 0.47 +0.1 4,107,321 - sbp b3917 0.47 -0.1 4,107,329 - sbp b3917 0.48 +1.2 4,107,339 - sbp b3917 0.49 +0.5 4,107,357 + sbp b3917 0.51 +0.3 4,107,357 + sbp b3917 0.51 +1.0 4,107,369 + sbp b3917 0.52 +1.0 4,107,369 + sbp b3917 0.52 +0.5 4,107,472 - sbp b3917 0.62 -1.2 4,107,502 + sbp b3917 0.65 +0.7
Or see this region's nucleotide sequence