Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhdJ and yhdU are separated by 83 nucleotides yhdU and envR are separated by 2 nucleotides envR and acrE are separated by 398 nucleotides
b3262: yhdJ - putative methyltransferase (VIMSS), at 3,409,675 to 3,410,559
yhdJ
b3263: yhdU - predicted membrane protein (NCBI), at 3,410,643 to 3,410,822
yhdU
b3264: envR - DNA-binding transcriptional regulator (NCBI), at 3,410,825 to 3,411,487
envR
b3265: acrE - cytoplasmic membrane lipoprotein (NCBI), at 3,411,886 to 3,413,043
acrE
Position (kb)
3410
3411
3412 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3409.828 kb on - strand, within yhdJ at 3409.873 kb on - strand, within yhdJ at 3409.879 kb on + strand, within yhdJ at 3409.879 kb on + strand, within yhdJ at 3409.895 kb on - strand, within yhdJ at 3409.915 kb on + strand, within yhdJ at 3409.968 kb on + strand, within yhdJ at 3410.048 kb on - strand, within yhdJ at 3410.097 kb on + strand, within yhdJ at 3410.108 kb on + strand, within yhdJ at 3410.168 kb on + strand, within yhdJ at 3410.186 kb on - strand, within yhdJ at 3410.186 kb on - strand, within yhdJ at 3410.316 kb on + strand, within yhdJ at 3410.334 kb on - strand, within yhdJ at 3410.423 kb on + strand, within yhdJ at 3410.460 kb on + strand, within yhdJ at 3410.464 kb on + strand, within yhdJ at 3410.565 kb on + strand at 3410.565 kb on + strand at 3410.565 kb on + strand at 3410.603 kb on + strand at 3410.613 kb on + strand at 3410.616 kb on - strand at 3410.618 kb on + strand at 3410.629 kb on + strand at 3410.631 kb on - strand at 3410.631 kb on - strand at 3410.633 kb on + strand at 3410.633 kb on + strand at 3410.633 kb on + strand at 3410.636 kb on - strand at 3410.653 kb on + strand at 3410.653 kb on + strand at 3410.654 kb on + strand at 3410.661 kb on - strand, within yhdU at 3410.677 kb on - strand, within yhdU at 3410.678 kb on - strand, within yhdU at 3410.678 kb on - strand, within yhdU at 3410.679 kb on + strand, within yhdU at 3410.693 kb on + strand, within yhdU at 3410.698 kb on + strand, within yhdU at 3410.712 kb on - strand, within yhdU at 3410.716 kb on + strand, within yhdU at 3410.716 kb on + strand, within yhdU at 3410.764 kb on + strand, within yhdU at 3410.764 kb on + strand, within yhdU at 3410.764 kb on + strand, within yhdU at 3410.772 kb on - strand, within yhdU at 3410.772 kb on - strand, within yhdU at 3410.778 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.788 kb on + strand, within yhdU at 3410.880 kb on + strand at 3410.896 kb on + strand, within envR at 3410.901 kb on + strand, within envR at 3410.911 kb on + strand, within envR at 3410.968 kb on - strand, within envR at 3411.043 kb on + strand, within envR at 3411.062 kb on + strand, within envR at 3411.079 kb on + strand, within envR at 3411.114 kb on + strand, within envR at 3411.122 kb on - strand, within envR at 3411.231 kb on - strand, within envR at 3411.243 kb on - strand, within envR at 3411.249 kb on - strand, within envR at 3411.250 kb on - strand, within envR at 3411.250 kb on - strand, within envR at 3411.252 kb on + strand, within envR at 3411.260 kb on - strand, within envR at 3411.260 kb on - strand, within envR at 3411.293 kb on + strand, within envR at 3411.298 kb on - strand, within envR at 3411.298 kb on - strand, within envR at 3411.306 kb on - strand, within envR at 3411.369 kb on - strand, within envR at 3411.397 kb on + strand, within envR at 3411.404 kb on + strand, within envR at 3411.410 kb on + strand, within envR at 3411.442 kb on + strand at 3411.442 kb on + strand at 3411.450 kb on + strand at 3411.452 kb on + strand at 3411.455 kb on + strand at 3411.807 kb on - strand at 3411.807 kb on - strand at 3411.807 kb on - strand at 3411.889 kb on - strand at 3411.891 kb on - strand at 3411.923 kb on - strand at 3411.928 kb on - strand at 3411.928 kb on - strand at 3411.928 kb on - strand at 3411.928 kb on - strand at 3411.947 kb on - strand at 3411.960 kb on + strand at 3411.960 kb on + strand at 3411.968 kb on - strand at 3411.999 kb on - strand at 3412.013 kb on + strand, within acrE at 3412.056 kb on - strand, within acrE at 3412.065 kb on + strand, within acrE at 3412.065 kb on + strand, within acrE at 3412.067 kb on - strand, within acrE at 3412.067 kb on - strand, within acrE at 3412.067 kb on - strand, within acrE at 3412.067 kb on - strand, within acrE at 3412.079 kb on - strand, within acrE at 3412.079 kb on - strand, within acrE at 3412.081 kb on - strand, within acrE at 3412.103 kb on - strand, within acrE at 3412.111 kb on - strand, within acrE at 3412.147 kb on - strand, within acrE at 3412.150 kb on + strand, within acrE at 3412.161 kb on + strand, within acrE at 3412.161 kb on + strand, within acrE at 3412.169 kb on - strand, within acrE at 3412.169 kb on - strand, within acrE at 3412.169 kb on - strand, within acrE at 3412.282 kb on + strand, within acrE at 3412.288 kb on + strand, within acrE at 3412.288 kb on + strand, within acrE at 3412.305 kb on + strand, within acrE at 3412.314 kb on - strand, within acrE at 3412.333 kb on + strand, within acrE
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 3,409,828 - yhdJ b3262 0.17 +0.1 3,409,873 - yhdJ b3262 0.22 -0.3 3,409,879 + yhdJ b3262 0.23 +0.6 3,409,879 + yhdJ b3262 0.23 -1.7 3,409,895 - yhdJ b3262 0.25 +0.7 3,409,915 + yhdJ b3262 0.27 -0.1 3,409,968 + yhdJ b3262 0.33 +1.3 3,410,048 - yhdJ b3262 0.42 -0.2 3,410,097 + yhdJ b3262 0.48 +1.1 3,410,108 + yhdJ b3262 0.49 +0.3 3,410,168 + yhdJ b3262 0.56 +0.0 3,410,186 - yhdJ b3262 0.58 -0.5 3,410,186 - yhdJ b3262 0.58 -2.0 3,410,316 + yhdJ b3262 0.72 +0.0 3,410,334 - yhdJ b3262 0.74 +2.7 3,410,423 + yhdJ b3262 0.85 +0.1 3,410,460 + yhdJ b3262 0.89 +0.7 3,410,464 + yhdJ b3262 0.89 -0.6 3,410,565 + +0.8 3,410,565 + +0.7 3,410,565 + -0.8 3,410,603 + +0.2 3,410,613 + -0.5 3,410,616 - +0.1 3,410,618 + +0.1 3,410,629 + -0.5 3,410,631 - +0.5 3,410,631 - +0.6 3,410,633 + -1.8 3,410,633 + +0.9 3,410,633 + +0.6 3,410,636 - -1.1 3,410,653 + +0.8 3,410,653 + +0.6 3,410,654 + +0.1 3,410,661 - yhdU b3263 0.10 +0.5 3,410,677 - yhdU b3263 0.19 +1.3 3,410,678 - yhdU b3263 0.19 +0.1 3,410,678 - yhdU b3263 0.19 +1.1 3,410,679 + yhdU b3263 0.20 -0.2 3,410,693 + yhdU b3263 0.28 -0.3 3,410,698 + yhdU b3263 0.31 +0.6 3,410,712 - yhdU b3263 0.38 +1.1 3,410,716 + yhdU b3263 0.41 +0.7 3,410,716 + yhdU b3263 0.41 -0.0 3,410,764 + yhdU b3263 0.67 +0.3 3,410,764 + yhdU b3263 0.67 -0.6 3,410,764 + yhdU b3263 0.67 +1.0 3,410,772 - yhdU b3263 0.72 +2.7 3,410,772 - yhdU b3263 0.72 +2.7 3,410,778 + yhdU b3263 0.75 -0.7 3,410,788 + yhdU b3263 0.81 +1.7 3,410,788 + yhdU b3263 0.81 +0.0 3,410,788 + yhdU b3263 0.81 +0.0 3,410,788 + yhdU b3263 0.81 -0.2 3,410,880 + -0.1 3,410,896 + envR b3264 0.11 -1.1 3,410,901 + envR b3264 0.11 +1.1 3,410,911 + envR b3264 0.13 -1.0 3,410,968 - envR b3264 0.22 +2.7 3,411,043 + envR b3264 0.33 +1.1 3,411,062 + envR b3264 0.36 +2.7 3,411,079 + envR b3264 0.38 +1.7 3,411,114 + envR b3264 0.44 +2.7 3,411,122 - envR b3264 0.45 +2.7 3,411,231 - envR b3264 0.61 -0.3 3,411,243 - envR b3264 0.63 +0.2 3,411,249 - envR b3264 0.64 +0.4 3,411,250 - envR b3264 0.64 -0.9 3,411,250 - envR b3264 0.64 -0.9 3,411,252 + envR b3264 0.64 +2.7 3,411,260 - envR b3264 0.66 +0.3 3,411,260 - envR b3264 0.66 -0.2 3,411,293 + envR b3264 0.71 +1.7 3,411,298 - envR b3264 0.71 -0.3 3,411,298 - envR b3264 0.71 -0.9 3,411,306 - envR b3264 0.73 +1.1 3,411,369 - envR b3264 0.82 -1.0 3,411,397 + envR b3264 0.86 +0.1 3,411,404 + envR b3264 0.87 -1.0 3,411,410 + envR b3264 0.88 +0.1 3,411,442 + +0.7 3,411,442 + -0.5 3,411,450 + +1.1 3,411,452 + +2.7 3,411,455 + +2.7 3,411,807 - -0.5 3,411,807 - +0.1 3,411,807 - +0.7 3,411,889 - -1.1 3,411,891 - +0.0 3,411,923 - +0.3 3,411,928 - +0.4 3,411,928 - -1.2 3,411,928 - +0.8 3,411,928 - -0.7 3,411,947 - +0.3 3,411,960 + +1.1 3,411,960 + +2.7 3,411,968 - -0.6 3,411,999 - -0.5 3,412,013 + acrE b3265 0.11 +1.2 3,412,056 - acrE b3265 0.15 -0.2 3,412,065 + acrE b3265 0.15 +0.3 3,412,065 + acrE b3265 0.15 +1.3 3,412,067 - acrE b3265 0.16 +0.2 3,412,067 - acrE b3265 0.16 +0.5 3,412,067 - acrE b3265 0.16 -0.1 3,412,067 - acrE b3265 0.16 -0.5 3,412,079 - acrE b3265 0.17 -0.3 3,412,079 - acrE b3265 0.17 +0.0 3,412,081 - acrE b3265 0.17 +0.8 3,412,103 - acrE b3265 0.19 +0.6 3,412,111 - acrE b3265 0.19 +0.3 3,412,147 - acrE b3265 0.23 +0.1 3,412,150 + acrE b3265 0.23 +0.3 3,412,161 + acrE b3265 0.24 -0.4 3,412,161 + acrE b3265 0.24 +0.7 3,412,169 - acrE b3265 0.24 -0.1 3,412,169 - acrE b3265 0.24 -0.0 3,412,169 - acrE b3265 0.24 -0.4 3,412,282 + acrE b3265 0.34 -0.1 3,412,288 + acrE b3265 0.35 +1.2 3,412,288 + acrE b3265 0.35 +0.6 3,412,305 + acrE b3265 0.36 -0.9 3,412,314 - acrE b3265 0.37 +0.4 3,412,333 + acrE b3265 0.39 -0.0
Or see this region's nucleotide sequence