Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycB and hycA are separated by 124 nucleotides hycA and hypA are separated by 211 nucleotides hypA and hypB are separated by 3 nucleotides hypB and hypC overlap by 10 nucleotides
b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871
hycB
b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457
hycA
b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019
hypA
b2727: hypB - GTP hydrolase involved in nickel liganding into hydrogenases (NCBI), at 2,849,023 to 2,849,895
hypB
b2728: hypC - protein required for maturation of hydrogenases 1 and 3 (NCBI), at 2,849,886 to 2,850,158
hypC
Position (kb)
2848
2849
2850 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2847.703 kb on + strand, within hycB at 2847.711 kb on - strand, within hycB at 2847.722 kb on - strand, within hycB at 2847.761 kb on - strand, within hycB at 2847.822 kb on - strand at 2847.829 kb on + strand at 2847.829 kb on + strand at 2847.888 kb on - strand at 2847.953 kb on - strand at 2848.071 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.082 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.719 kb on - strand, within hypA at 2848.850 kb on - strand, within hypA at 2848.892 kb on + strand, within hypA at 2848.912 kb on + strand, within hypA at 2848.944 kb on + strand, within hypA at 2848.965 kb on - strand, within hypA at 2848.965 kb on - strand, within hypA at 2849.025 kb on + strand at 2849.025 kb on + strand at 2849.290 kb on - strand, within hypB at 2849.290 kb on - strand, within hypB at 2849.345 kb on + strand, within hypB at 2849.390 kb on - strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.486 kb on + strand, within hypB at 2849.486 kb on + strand, within hypB at 2849.494 kb on + strand, within hypB at 2849.507 kb on - strand, within hypB at 2849.581 kb on - strand, within hypB at 2849.748 kb on + strand, within hypB at 2849.884 kb on - strand at 2849.917 kb on + strand, within hypC at 2849.917 kb on + strand, within hypC at 2849.917 kb on - strand, within hypC at 2849.917 kb on - strand, within hypC
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 2,847,703 + hycB b2724 0.72 +0.4 2,847,711 - hycB b2724 0.74 +0.1 2,847,722 - hycB b2724 0.75 -0.8 2,847,761 - hycB b2724 0.82 +0.9 2,847,822 - -0.7 2,847,829 + +0.5 2,847,829 + +0.1 2,847,888 - -0.7 2,847,953 - +0.2 2,848,071 - hycA b2725 0.16 -3.9 2,848,073 - hycA b2725 0.17 -0.2 2,848,073 - hycA b2725 0.17 -0.2 2,848,082 - hycA b2725 0.19 -0.4 2,848,153 - hycA b2725 0.34 +0.3 2,848,153 - hycA b2725 0.34 +0.2 2,848,157 + hycA b2725 0.35 +0.8 2,848,157 + hycA b2725 0.35 -0.4 2,848,253 - hycA b2725 0.56 -0.7 2,848,253 - hycA b2725 0.56 -0.4 2,848,684 - +0.2 2,848,684 - +0.1 2,848,684 - +0.4 2,848,719 - hypA b2726 0.14 +0.3 2,848,850 - hypA b2726 0.52 +0.7 2,848,892 + hypA b2726 0.64 +0.5 2,848,912 + hypA b2726 0.69 -0.2 2,848,944 + hypA b2726 0.78 +0.9 2,848,965 - hypA b2726 0.84 +0.6 2,848,965 - hypA b2726 0.84 -0.0 2,849,025 + +0.0 2,849,025 + -0.5 2,849,290 - hypB b2727 0.31 -0.0 2,849,290 - hypB b2727 0.31 -0.1 2,849,345 + hypB b2727 0.37 +0.4 2,849,390 - hypB b2727 0.42 -0.2 2,849,430 + hypB b2727 0.47 +1.6 2,849,430 + hypB b2727 0.47 +0.4 2,849,486 + hypB b2727 0.53 -0.4 2,849,486 + hypB b2727 0.53 -0.9 2,849,494 + hypB b2727 0.54 -1.0 2,849,507 - hypB b2727 0.55 +0.4 2,849,581 - hypB b2727 0.64 +0.3 2,849,748 + hypB b2727 0.83 -0.6 2,849,884 - -0.7 2,849,917 + hypC b2728 0.11 +0.7 2,849,917 + hypC b2728 0.11 -0.4 2,849,917 - hypC b2728 0.11 -0.1 2,849,917 - hypC b2728 0.11 +0.0
Or see this region's nucleotide sequence