Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfR and focB are separated by 21 nucleotides focB and yfgO are separated by 37 nucleotides
b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934
hyfR
b2492: focB - predicted formate transporter (NCBI), at 2,611,956 to 2,612,804
focB
b2493: yfgO - predicted inner membrane protein (NCBI), at 2,612,842 to 2,613,903
yfgO
Position (kb)
2611
2612
2613 Strain fitness (log2 ratio)
-1
0
1
2 at 2611.065 kb on + strand, within hyfR at 2611.090 kb on + strand, within hyfR at 2611.139 kb on - strand, within hyfR at 2611.139 kb on - strand, within hyfR at 2611.289 kb on + strand, within hyfR at 2611.392 kb on - strand, within hyfR at 2611.599 kb on - strand, within hyfR at 2611.599 kb on - strand, within hyfR at 2611.829 kb on - strand at 2611.950 kb on + strand at 2612.067 kb on - strand, within focB at 2612.067 kb on - strand, within focB at 2612.133 kb on - strand, within focB at 2612.145 kb on + strand, within focB at 2612.214 kb on + strand, within focB at 2612.214 kb on + strand, within focB at 2612.217 kb on - strand, within focB at 2612.217 kb on - strand, within focB at 2612.397 kb on + strand, within focB at 2612.425 kb on + strand, within focB at 2612.497 kb on - strand, within focB at 2612.579 kb on + strand, within focB at 2612.579 kb on + strand, within focB at 2612.589 kb on - strand, within focB at 2612.690 kb on - strand, within focB at 2612.690 kb on - strand, within focB at 2612.720 kb on - strand at 2612.720 kb on - strand at 2612.749 kb on + strand at 2612.749 kb on + strand at 2612.764 kb on + strand at 2612.764 kb on + strand at 2612.845 kb on - strand at 2612.845 kb on - strand at 2612.875 kb on - strand at 2612.875 kb on - strand at 2612.958 kb on - strand, within yfgO at 2612.986 kb on - strand, within yfgO at 2612.986 kb on - strand, within yfgO at 2613.179 kb on - strand, within yfgO at 2613.179 kb on - strand, within yfgO at 2613.183 kb on + strand, within yfgO at 2613.309 kb on - strand, within yfgO at 2613.313 kb on - strand, within yfgO at 2613.313 kb on - strand, within yfgO at 2613.320 kb on + strand, within yfgO at 2613.402 kb on + strand, within yfgO at 2613.402 kb on + strand, within yfgO at 2613.424 kb on - strand, within yfgO at 2613.629 kb on + strand, within yfgO at 2613.642 kb on - strand, within yfgO at 2613.642 kb on - strand, within yfgO at 2613.668 kb on - strand, within yfgO at 2613.668 kb on - strand, within yfgO at 2613.678 kb on + strand, within yfgO at 2613.750 kb on + strand, within yfgO
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 2,611,065 + hyfR b2491 0.57 -1.7 2,611,090 + hyfR b2491 0.58 +0.3 2,611,139 - hyfR b2491 0.60 -0.5 2,611,139 - hyfR b2491 0.60 +0.5 2,611,289 + hyfR b2491 0.68 -0.2 2,611,392 - hyfR b2491 0.73 -0.8 2,611,599 - hyfR b2491 0.83 +0.3 2,611,599 - hyfR b2491 0.83 -0.2 2,611,829 - +0.6 2,611,950 + -0.6 2,612,067 - focB b2492 0.13 +0.0 2,612,067 - focB b2492 0.13 -0.3 2,612,133 - focB b2492 0.21 +0.2 2,612,145 + focB b2492 0.22 +0.3 2,612,214 + focB b2492 0.30 +0.6 2,612,214 + focB b2492 0.30 +1.6 2,612,217 - focB b2492 0.31 +1.1 2,612,217 - focB b2492 0.31 +0.1 2,612,397 + focB b2492 0.52 +0.4 2,612,425 + focB b2492 0.55 +2.3 2,612,497 - focB b2492 0.64 -0.3 2,612,579 + focB b2492 0.73 +0.8 2,612,579 + focB b2492 0.73 +0.9 2,612,589 - focB b2492 0.75 -0.3 2,612,690 - focB b2492 0.86 +0.5 2,612,690 - focB b2492 0.86 +0.5 2,612,720 - +0.4 2,612,720 - +0.6 2,612,749 + -0.4 2,612,749 + +0.9 2,612,764 + +0.3 2,612,764 + +0.7 2,612,845 - -0.1 2,612,845 - +0.6 2,612,875 - -1.3 2,612,875 - -0.6 2,612,958 - yfgO b2493 0.11 -0.2 2,612,986 - yfgO b2493 0.14 +0.9 2,612,986 - yfgO b2493 0.14 -0.0 2,613,179 - yfgO b2493 0.32 +1.8 2,613,179 - yfgO b2493 0.32 +0.1 2,613,183 + yfgO b2493 0.32 +0.6 2,613,309 - yfgO b2493 0.44 -0.9 2,613,313 - yfgO b2493 0.44 -0.3 2,613,313 - yfgO b2493 0.44 -0.5 2,613,320 + yfgO b2493 0.45 -0.7 2,613,402 + yfgO b2493 0.53 -0.9 2,613,402 + yfgO b2493 0.53 -0.5 2,613,424 - yfgO b2493 0.55 -1.0 2,613,629 + yfgO b2493 0.74 +0.1 2,613,642 - yfgO b2493 0.75 -0.1 2,613,642 - yfgO b2493 0.75 -0.9 2,613,668 - yfgO b2493 0.78 +0.5 2,613,668 - yfgO b2493 0.78 +0.7 2,613,678 + yfgO b2493 0.79 -0.8 2,613,750 + yfgO b2493 0.85 +0.5
Or see this region's nucleotide sequence