Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpsB and wcaI are separated by 2 nucleotides wcaI and wcaH overlap by 4 nucleotides wcaH and fcl are separated by 2 nucleotides
b2049: cpsB - mannose-1-phosphate guanyltransferase (NCBI), at 2,121,108 to 2,122,544
cpsB
b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770
wcaI
b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246
wcaH
b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214
fcl
Position (kb)
2122
2123
2124 Strain fitness (log2 ratio)
-1
0
1 at 2121.605 kb on + strand, within cpsB at 2121.742 kb on + strand, within cpsB at 2121.742 kb on + strand, within cpsB at 2121.969 kb on + strand, within cpsB at 2122.061 kb on + strand, within cpsB at 2122.061 kb on + strand, within cpsB at 2122.748 kb on + strand, within wcaI at 2122.748 kb on + strand, within wcaI at 2123.290 kb on + strand, within wcaI at 2123.290 kb on + strand, within wcaI at 2123.479 kb on + strand, within wcaI at 2123.610 kb on + strand, within wcaI at 2123.668 kb on + strand at 2123.822 kb on + strand, within wcaH at 2124.177 kb on - strand, within wcaH at 2124.282 kb on + strand at 2124.282 kb on + strand at 2124.465 kb on + strand, within fcl at 2124.601 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.722 kb on - strand, within fcl
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 2,121,605 + cpsB b2049 0.35 -0.0 2,121,742 + cpsB b2049 0.44 -0.2 2,121,742 + cpsB b2049 0.44 -0.1 2,121,969 + cpsB b2049 0.60 +0.0 2,122,061 + cpsB b2049 0.66 +0.3 2,122,061 + cpsB b2049 0.66 +1.3 2,122,748 + wcaI b2050 0.16 +0.4 2,122,748 + wcaI b2050 0.16 +0.5 2,123,290 + wcaI b2050 0.61 +0.7 2,123,290 + wcaI b2050 0.61 +1.5 2,123,479 + wcaI b2050 0.76 +0.3 2,123,610 + wcaI b2050 0.87 +0.1 2,123,668 + +0.3 2,123,822 + wcaH b2051 0.11 +0.0 2,124,177 - wcaH b2051 0.85 +0.1 2,124,282 + +0.6 2,124,282 + +0.2 2,124,465 + fcl b2052 0.22 -0.2 2,124,601 + fcl b2052 0.36 +0.4 2,124,630 + fcl b2052 0.39 -0.7 2,124,630 + fcl b2052 0.39 -0.5 2,124,722 - fcl b2052 0.49 +0.5
Or see this region's nucleotide sequence