Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wbbK and wbbJ overlap by 16 nucleotides wbbJ and wbbI overlap by 20 nucleotides wbbI and wbbH are separated by 2 nucleotides
b2032: wbbK - lipopolysaccharide biosynthesis protein (NCBI), at 2,101,415 to 2,102,533
wbbK
b2033: wbbJ - predicted acyl transferase (NCBI), at 2,102,518 to 2,103,108
wbbJ
b2034: wbbI - hypothetical protein (NCBI), at 2,103,089 to 2,104,081
wbbI
b2035: wbbH - O-antigen polymerase (NCBI), at 2,104,084 to 2,105,250
wbbH
Position (kb)
2102
2103
2104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2101.600 kb on - strand, within wbbK at 2101.637 kb on - strand, within wbbK at 2101.638 kb on - strand, within wbbK at 2101.651 kb on - strand, within wbbK at 2101.657 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.659 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.670 kb on - strand, within wbbK at 2101.672 kb on - strand, within wbbK at 2101.791 kb on - strand, within wbbK at 2101.827 kb on - strand, within wbbK at 2101.827 kb on - strand, within wbbK at 2101.832 kb on - strand, within wbbK at 2101.832 kb on - strand, within wbbK at 2101.833 kb on - strand, within wbbK at 2101.921 kb on - strand, within wbbK at 2101.921 kb on - strand, within wbbK at 2101.950 kb on - strand, within wbbK at 2101.961 kb on - strand, within wbbK at 2102.023 kb on - strand, within wbbK at 2102.023 kb on - strand, within wbbK at 2102.032 kb on - strand, within wbbK at 2102.134 kb on - strand, within wbbK at 2102.134 kb on - strand, within wbbK at 2102.166 kb on - strand, within wbbK at 2102.166 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.211 kb on - strand, within wbbK at 2102.242 kb on - strand, within wbbK at 2102.242 kb on - strand, within wbbK at 2102.253 kb on - strand, within wbbK at 2102.280 kb on - strand, within wbbK at 2102.350 kb on - strand, within wbbK at 2102.351 kb on - strand, within wbbK at 2102.358 kb on - strand, within wbbK at 2102.406 kb on - strand, within wbbK at 2102.414 kb on - strand, within wbbK at 2102.418 kb on - strand, within wbbK at 2102.500 kb on - strand at 2102.587 kb on - strand, within wbbJ at 2102.589 kb on - strand, within wbbJ at 2102.640 kb on - strand, within wbbJ at 2102.686 kb on - strand, within wbbJ at 2102.694 kb on - strand, within wbbJ at 2102.702 kb on - strand, within wbbJ at 2102.769 kb on - strand, within wbbJ at 2102.769 kb on - strand, within wbbJ at 2102.783 kb on - strand, within wbbJ at 2102.828 kb on - strand, within wbbJ at 2102.828 kb on - strand, within wbbJ at 2102.833 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.848 kb on - strand, within wbbJ at 2102.871 kb on - strand, within wbbJ at 2102.876 kb on - strand, within wbbJ at 2102.933 kb on - strand, within wbbJ at 2102.939 kb on - strand, within wbbJ at 2102.939 kb on - strand, within wbbJ at 2102.939 kb on - strand, within wbbJ at 2102.996 kb on - strand, within wbbJ at 2103.057 kb on - strand at 2103.059 kb on - strand at 2103.116 kb on - strand at 2103.126 kb on - strand at 2103.126 kb on - strand at 2103.126 kb on - strand at 2103.137 kb on - strand at 2103.215 kb on - strand, within wbbI at 2103.215 kb on - strand, within wbbI at 2103.215 kb on - strand, within wbbI at 2103.215 kb on - strand, within wbbI at 2103.242 kb on - strand, within wbbI at 2103.251 kb on - strand, within wbbI at 2103.251 kb on - strand, within wbbI at 2103.252 kb on - strand, within wbbI at 2103.349 kb on - strand, within wbbI at 2103.362 kb on - strand, within wbbI at 2103.382 kb on - strand, within wbbI at 2103.382 kb on - strand, within wbbI at 2103.392 kb on - strand, within wbbI at 2103.393 kb on - strand, within wbbI at 2103.436 kb on - strand, within wbbI at 2103.436 kb on - strand, within wbbI at 2103.449 kb on - strand, within wbbI at 2103.490 kb on - strand, within wbbI at 2103.490 kb on - strand, within wbbI at 2103.592 kb on - strand, within wbbI at 2103.599 kb on - strand, within wbbI at 2103.603 kb on - strand, within wbbI at 2103.613 kb on - strand, within wbbI at 2103.613 kb on - strand, within wbbI at 2103.623 kb on - strand, within wbbI at 2103.625 kb on - strand, within wbbI at 2103.628 kb on - strand, within wbbI at 2103.635 kb on - strand, within wbbI at 2103.635 kb on - strand, within wbbI at 2103.640 kb on - strand, within wbbI at 2103.689 kb on - strand, within wbbI at 2103.717 kb on - strand, within wbbI at 2103.733 kb on - strand, within wbbI at 2103.741 kb on - strand, within wbbI at 2103.741 kb on - strand, within wbbI at 2103.742 kb on - strand, within wbbI at 2103.742 kb on - strand, within wbbI at 2103.754 kb on - strand, within wbbI at 2103.754 kb on - strand, within wbbI at 2103.759 kb on - strand, within wbbI at 2103.764 kb on - strand, within wbbI at 2103.768 kb on - strand, within wbbI at 2103.770 kb on - strand, within wbbI at 2103.770 kb on - strand, within wbbI at 2103.777 kb on - strand, within wbbI at 2103.777 kb on - strand, within wbbI at 2103.813 kb on - strand, within wbbI at 2103.933 kb on - strand, within wbbI at 2103.933 kb on - strand, within wbbI at 2103.952 kb on - strand, within wbbI at 2103.953 kb on - strand, within wbbI at 2103.953 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.962 kb on - strand, within wbbI at 2103.963 kb on - strand, within wbbI at 2103.975 kb on - strand, within wbbI at 2104.000 kb on - strand at 2104.008 kb on - strand at 2104.008 kb on - strand at 2104.008 kb on - strand at 2104.008 kb on - strand at 2104.039 kb on - strand at 2104.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 2,101,600 - wbbK b2032 0.17 -1.4 2,101,637 - wbbK b2032 0.20 +0.5 2,101,638 - wbbK b2032 0.20 +0.0 2,101,651 - wbbK b2032 0.21 -0.1 2,101,657 - wbbK b2032 0.22 +1.0 2,101,659 - wbbK b2032 0.22 -0.4 2,101,659 - wbbK b2032 0.22 -1.4 2,101,659 - wbbK b2032 0.22 -1.0 2,101,659 - wbbK b2032 0.22 +0.2 2,101,659 - wbbK b2032 0.22 +0.8 2,101,670 - wbbK b2032 0.23 -1.1 2,101,670 - wbbK b2032 0.23 +0.9 2,101,670 - wbbK b2032 0.23 +0.9 2,101,670 - wbbK b2032 0.23 +2.3 2,101,670 - wbbK b2032 0.23 +0.4 2,101,672 - wbbK b2032 0.23 +0.3 2,101,791 - wbbK b2032 0.34 +0.2 2,101,827 - wbbK b2032 0.37 +1.6 2,101,827 - wbbK b2032 0.37 -1.6 2,101,832 - wbbK b2032 0.37 -1.9 2,101,832 - wbbK b2032 0.37 -0.3 2,101,833 - wbbK b2032 0.37 -0.4 2,101,921 - wbbK b2032 0.45 +0.4 2,101,921 - wbbK b2032 0.45 -0.9 2,101,950 - wbbK b2032 0.48 +1.1 2,101,961 - wbbK b2032 0.49 -0.3 2,102,023 - wbbK b2032 0.54 +0.9 2,102,023 - wbbK b2032 0.54 -0.5 2,102,032 - wbbK b2032 0.55 +0.9 2,102,134 - wbbK b2032 0.64 +0.3 2,102,134 - wbbK b2032 0.64 -0.6 2,102,166 - wbbK b2032 0.67 +1.2 2,102,166 - wbbK b2032 0.67 +0.5 2,102,211 - wbbK b2032 0.71 +0.6 2,102,211 - wbbK b2032 0.71 -0.9 2,102,211 - wbbK b2032 0.71 +1.6 2,102,211 - wbbK b2032 0.71 -0.4 2,102,242 - wbbK b2032 0.74 +0.3 2,102,242 - wbbK b2032 0.74 -0.3 2,102,253 - wbbK b2032 0.75 -1.2 2,102,280 - wbbK b2032 0.77 -0.5 2,102,350 - wbbK b2032 0.84 +0.6 2,102,351 - wbbK b2032 0.84 -0.6 2,102,358 - wbbK b2032 0.84 +0.9 2,102,406 - wbbK b2032 0.89 -0.0 2,102,414 - wbbK b2032 0.89 +1.8 2,102,418 - wbbK b2032 0.90 -0.4 2,102,500 - -0.2 2,102,587 - wbbJ b2033 0.12 -0.5 2,102,589 - wbbJ b2033 0.12 -1.8 2,102,640 - wbbJ b2033 0.21 -0.1 2,102,686 - wbbJ b2033 0.28 +0.6 2,102,694 - wbbJ b2033 0.30 +0.6 2,102,702 - wbbJ b2033 0.31 +0.6 2,102,769 - wbbJ b2033 0.42 +1.5 2,102,769 - wbbJ b2033 0.42 +1.7 2,102,783 - wbbJ b2033 0.45 +0.0 2,102,828 - wbbJ b2033 0.52 +0.2 2,102,828 - wbbJ b2033 0.52 -1.5 2,102,833 - wbbJ b2033 0.53 -1.0 2,102,848 - wbbJ b2033 0.56 +0.5 2,102,848 - wbbJ b2033 0.56 +1.1 2,102,848 - wbbJ b2033 0.56 +1.4 2,102,848 - wbbJ b2033 0.56 +1.0 2,102,848 - wbbJ b2033 0.56 +0.0 2,102,848 - wbbJ b2033 0.56 +0.8 2,102,848 - wbbJ b2033 0.56 +0.2 2,102,871 - wbbJ b2033 0.60 +1.1 2,102,876 - wbbJ b2033 0.61 -0.2 2,102,933 - wbbJ b2033 0.70 +0.8 2,102,939 - wbbJ b2033 0.71 -0.9 2,102,939 - wbbJ b2033 0.71 -1.0 2,102,939 - wbbJ b2033 0.71 +1.0 2,102,996 - wbbJ b2033 0.81 +0.8 2,103,057 - -0.3 2,103,059 - +0.2 2,103,116 - +1.3 2,103,126 - +0.9 2,103,126 - -0.1 2,103,126 - -0.4 2,103,137 - -0.5 2,103,215 - wbbI b2034 0.13 -0.2 2,103,215 - wbbI b2034 0.13 -1.0 2,103,215 - wbbI b2034 0.13 +0.2 2,103,215 - wbbI b2034 0.13 +0.0 2,103,242 - wbbI b2034 0.15 +1.1 2,103,251 - wbbI b2034 0.16 +0.6 2,103,251 - wbbI b2034 0.16 -0.1 2,103,252 - wbbI b2034 0.16 -0.3 2,103,349 - wbbI b2034 0.26 -0.4 2,103,362 - wbbI b2034 0.27 -0.4 2,103,382 - wbbI b2034 0.30 +0.8 2,103,382 - wbbI b2034 0.30 +0.3 2,103,392 - wbbI b2034 0.31 -0.2 2,103,393 - wbbI b2034 0.31 -1.2 2,103,436 - wbbI b2034 0.35 -0.0 2,103,436 - wbbI b2034 0.35 -0.3 2,103,449 - wbbI b2034 0.36 +1.3 2,103,490 - wbbI b2034 0.40 +0.6 2,103,490 - wbbI b2034 0.40 +0.4 2,103,592 - wbbI b2034 0.51 +0.3 2,103,599 - wbbI b2034 0.51 -0.3 2,103,603 - wbbI b2034 0.52 -0.3 2,103,613 - wbbI b2034 0.53 +0.9 2,103,613 - wbbI b2034 0.53 +1.1 2,103,623 - wbbI b2034 0.54 -0.3 2,103,625 - wbbI b2034 0.54 +0.5 2,103,628 - wbbI b2034 0.54 +0.2 2,103,635 - wbbI b2034 0.55 -1.4 2,103,635 - wbbI b2034 0.55 +0.4 2,103,640 - wbbI b2034 0.55 -0.4 2,103,689 - wbbI b2034 0.60 -0.1 2,103,717 - wbbI b2034 0.63 -0.6 2,103,733 - wbbI b2034 0.65 +0.1 2,103,741 - wbbI b2034 0.66 +0.9 2,103,741 - wbbI b2034 0.66 -0.6 2,103,742 - wbbI b2034 0.66 +1.5 2,103,742 - wbbI b2034 0.66 +1.9 2,103,754 - wbbI b2034 0.67 +1.2 2,103,754 - wbbI b2034 0.67 +0.3 2,103,759 - wbbI b2034 0.67 +0.9 2,103,764 - wbbI b2034 0.68 +0.4 2,103,768 - wbbI b2034 0.68 +0.8 2,103,770 - wbbI b2034 0.69 +0.3 2,103,770 - wbbI b2034 0.69 +0.4 2,103,777 - wbbI b2034 0.69 +1.4 2,103,777 - wbbI b2034 0.69 +0.4 2,103,813 - wbbI b2034 0.73 +0.2 2,103,933 - wbbI b2034 0.85 -0.0 2,103,933 - wbbI b2034 0.85 +1.3 2,103,952 - wbbI b2034 0.87 +1.5 2,103,953 - wbbI b2034 0.87 +0.4 2,103,953 - wbbI b2034 0.87 -1.3 2,103,962 - wbbI b2034 0.88 +0.8 2,103,962 - wbbI b2034 0.88 -0.9 2,103,962 - wbbI b2034 0.88 -0.5 2,103,962 - wbbI b2034 0.88 +1.0 2,103,962 - wbbI b2034 0.88 -0.3 2,103,962 - wbbI b2034 0.88 +0.9 2,103,962 - wbbI b2034 0.88 +0.6 2,103,963 - wbbI b2034 0.88 -0.1 2,103,975 - wbbI b2034 0.89 -1.3 2,104,000 - -2.4 2,104,008 - -0.9 2,104,008 - -0.2 2,104,008 - +0.6 2,104,008 - +1.2 2,104,039 - -0.1 2,104,039 - +0.3
Or see this region's nucleotide sequence