Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedO and fliY are separated by 104 nucleotides fliY and fliZ are separated by 87 nucleotides fliZ and fliA are separated by 45 nucleotides
b1919: yedO - putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS), at 1,996,518 to 1,997,504
yedO
b1920: fliY - cystine transporter subunit (NCBI), at 1,997,609 to 1,998,409
fliY
b1921: fliZ - orf, hypothetical protein (VIMSS), at 1,998,497 to 1,999,048
fliZ
b1922: fliA - flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS), at 1,999,094 to 1,999,813
fliA
Position (kb)
1998
1999
2000 Strain fitness (log2 ratio)
-2
-1
0
1 at 1997.658 kb on + strand at 1997.811 kb on + strand, within fliY at 1997.811 kb on + strand, within fliY at 1997.931 kb on - strand, within fliY at 1998.266 kb on + strand, within fliY at 1998.375 kb on + strand at 1998.375 kb on + strand at 1998.387 kb on - strand at 1998.549 kb on - strand at 1998.568 kb on + strand, within fliZ at 1998.568 kb on - strand, within fliZ at 1998.616 kb on - strand, within fliZ at 1998.981 kb on + strand, within fliZ at 1998.981 kb on + strand, within fliZ at 1999.139 kb on + strand at 1999.145 kb on - strand at 1999.145 kb on - strand at 1999.177 kb on - strand, within fliA at 1999.209 kb on - strand, within fliA at 1999.292 kb on - strand, within fliA at 1999.292 kb on - strand, within fliA at 1999.302 kb on - strand, within fliA at 1999.504 kb on - strand, within fliA at 1999.504 kb on - strand, within fliA at 1999.508 kb on - strand, within fliA at 1999.509 kb on - strand, within fliA at 1999.509 kb on - strand, within fliA at 1999.541 kb on + strand, within fliA at 1999.595 kb on - strand, within fliA at 1999.600 kb on - strand, within fliA at 1999.615 kb on + strand, within fliA at 1999.655 kb on + strand, within fliA at 1999.800 kb on - strand at 1999.832 kb on + strand at 1999.903 kb on - strand at 1999.969 kb on + strand at 1999.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,997,658 + -0.9 1,997,811 + fliY b1920 0.25 -0.4 1,997,811 + fliY b1920 0.25 +0.2 1,997,931 - fliY b1920 0.40 +0.4 1,998,266 + fliY b1920 0.82 +0.1 1,998,375 + -0.5 1,998,375 + -1.3 1,998,387 - +0.3 1,998,549 - -0.9 1,998,568 + fliZ b1921 0.13 -1.9 1,998,568 - fliZ b1921 0.13 -0.5 1,998,616 - fliZ b1921 0.22 -0.2 1,998,981 + fliZ b1921 0.88 -1.2 1,998,981 + fliZ b1921 0.88 +0.4 1,999,139 + -1.2 1,999,145 - -0.7 1,999,145 - +1.3 1,999,177 - fliA b1922 0.12 +0.5 1,999,209 - fliA b1922 0.16 -0.5 1,999,292 - fliA b1922 0.28 -0.2 1,999,292 - fliA b1922 0.28 -1.4 1,999,302 - fliA b1922 0.29 +0.5 1,999,504 - fliA b1922 0.57 +0.9 1,999,504 - fliA b1922 0.57 -0.1 1,999,508 - fliA b1922 0.57 +0.2 1,999,509 - fliA b1922 0.58 +0.1 1,999,509 - fliA b1922 0.58 +0.5 1,999,541 + fliA b1922 0.62 +0.4 1,999,595 - fliA b1922 0.70 +0.4 1,999,600 - fliA b1922 0.70 +0.7 1,999,615 + fliA b1922 0.72 -0.2 1,999,655 + fliA b1922 0.78 +0.4 1,999,800 - -0.2 1,999,832 + -0.5 1,999,903 - +0.2 1,999,969 + +0.4 1,999,969 + -0.4
Or see this region's nucleotide sequence