Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydiK and rprA are separated by 185 nucleotides rprA and b1689 are separated by 137 nucleotides b1689 and ydiM are separated by 99 nucleotides ydiM and ydiN are separated by 226 nucleotides
b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210
ydiK
b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501
rprA
b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995
b1689
b1690: ydiM - predicted transporter (NCBI), at 1,769,095 to 1,770,309
ydiM
b1691: ydiN - predicted transporter (RefSeq), at 1,770,536 to 1,771,801
ydiN
Position (kb)
1769
1770
1771 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1768.225 kb on + strand at 1768.225 kb on + strand at 1768.227 kb on + strand at 1768.290 kb on - strand at 1768.307 kb on + strand at 1768.307 kb on + strand at 1768.312 kb on - strand at 1768.382 kb on + strand at 1768.390 kb on - strand at 1768.466 kb on - strand, within rprA at 1768.471 kb on - strand, within rprA at 1768.479 kb on + strand, within rprA at 1768.516 kb on + strand at 1768.551 kb on + strand at 1768.559 kb on - strand at 1768.561 kb on + strand at 1768.561 kb on + strand at 1768.569 kb on + strand at 1768.569 kb on + strand at 1768.569 kb on - strand at 1768.571 kb on + strand at 1768.654 kb on + strand at 1768.661 kb on - strand at 1768.685 kb on + strand, within b1689 at 1768.714 kb on - strand, within b1689 at 1768.720 kb on + strand, within b1689 at 1768.765 kb on + strand, within b1689 at 1768.765 kb on + strand, within b1689 at 1768.781 kb on - strand, within b1689 at 1768.788 kb on + strand, within b1689 at 1768.788 kb on + strand, within b1689 at 1768.905 kb on + strand, within b1689 at 1768.905 kb on + strand, within b1689 at 1768.905 kb on + strand, within b1689 at 1768.913 kb on - strand, within b1689 at 1768.976 kb on + strand at 1769.159 kb on - strand at 1769.186 kb on - strand at 1769.198 kb on + strand at 1769.223 kb on + strand, within ydiM at 1769.230 kb on + strand, within ydiM at 1769.238 kb on - strand, within ydiM at 1769.244 kb on + strand, within ydiM at 1769.244 kb on + strand, within ydiM at 1769.323 kb on + strand, within ydiM at 1769.331 kb on - strand, within ydiM at 1769.341 kb on - strand, within ydiM at 1769.401 kb on + strand, within ydiM at 1769.461 kb on + strand, within ydiM at 1769.464 kb on + strand, within ydiM at 1769.548 kb on - strand, within ydiM at 1769.556 kb on - strand, within ydiM at 1769.561 kb on - strand, within ydiM at 1769.566 kb on - strand, within ydiM at 1769.641 kb on - strand, within ydiM at 1769.646 kb on + strand, within ydiM at 1769.653 kb on - strand, within ydiM at 1769.751 kb on - strand, within ydiM at 1769.770 kb on - strand, within ydiM at 1769.791 kb on - strand, within ydiM at 1769.817 kb on + strand, within ydiM at 1769.817 kb on - strand, within ydiM at 1769.847 kb on - strand, within ydiM at 1769.847 kb on - strand, within ydiM at 1769.942 kb on - strand, within ydiM at 1769.942 kb on - strand, within ydiM at 1769.951 kb on + strand, within ydiM at 1770.096 kb on + strand, within ydiM at 1770.106 kb on + strand, within ydiM at 1770.858 kb on - strand, within ydiN at 1770.860 kb on - strand, within ydiN at 1770.860 kb on - strand, within ydiN at 1770.870 kb on - strand, within ydiN at 1770.870 kb on - strand, within ydiN at 1770.870 kb on - strand, within ydiN at 1770.882 kb on - strand, within ydiN at 1770.906 kb on - strand, within ydiN at 1771.124 kb on + strand, within ydiN at 1771.160 kb on + strand, within ydiN at 1771.195 kb on + strand, within ydiN at 1771.269 kb on + strand, within ydiN at 1771.273 kb on + strand, within ydiN at 1771.273 kb on + strand, within ydiN at 1771.273 kb on + strand, within ydiN
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,768,225 + +0.6 1,768,225 + +2.2 1,768,227 + +0.5 1,768,290 - +0.3 1,768,307 + +0.4 1,768,307 + +0.0 1,768,312 - +0.3 1,768,382 + +0.2 1,768,390 - -0.3 1,768,466 - rprA b4431 0.66 +0.4 1,768,471 - rprA b4431 0.71 +0.6 1,768,479 + rprA b4431 0.78 -1.4 1,768,516 + +1.6 1,768,551 + -1.2 1,768,559 - +0.4 1,768,561 + -1.1 1,768,561 + +0.1 1,768,569 + +0.3 1,768,569 + +0.1 1,768,569 - -0.3 1,768,571 + -1.1 1,768,654 + +0.6 1,768,661 - +0.1 1,768,685 + b1689 b1689 0.13 -1.7 1,768,714 - b1689 b1689 0.21 +0.4 1,768,720 + b1689 b1689 0.23 +0.2 1,768,765 + b1689 b1689 0.35 +3.0 1,768,765 + b1689 b1689 0.35 +1.2 1,768,781 - b1689 b1689 0.40 -0.7 1,768,788 + b1689 b1689 0.42 -0.1 1,768,788 + b1689 b1689 0.42 -2.4 1,768,905 + b1689 b1689 0.75 +2.4 1,768,905 + b1689 b1689 0.75 +0.6 1,768,905 + b1689 b1689 0.75 +0.3 1,768,913 - b1689 b1689 0.77 +1.6 1,768,976 + +1.0 1,769,159 - +1.2 1,769,186 - +0.6 1,769,198 + +1.6 1,769,223 + ydiM b1690 0.11 -0.2 1,769,230 + ydiM b1690 0.11 -0.4 1,769,238 - ydiM b1690 0.12 +1.6 1,769,244 + ydiM b1690 0.12 -0.4 1,769,244 + ydiM b1690 0.12 +0.1 1,769,323 + ydiM b1690 0.19 -0.7 1,769,331 - ydiM b1690 0.19 +1.6 1,769,341 - ydiM b1690 0.20 +1.6 1,769,401 + ydiM b1690 0.25 +1.6 1,769,461 + ydiM b1690 0.30 -0.7 1,769,464 + ydiM b1690 0.30 -1.2 1,769,548 - ydiM b1690 0.37 -1.4 1,769,556 - ydiM b1690 0.38 +1.6 1,769,561 - ydiM b1690 0.38 -1.2 1,769,566 - ydiM b1690 0.39 +2.6 1,769,641 - ydiM b1690 0.45 +0.1 1,769,646 + ydiM b1690 0.45 +1.6 1,769,653 - ydiM b1690 0.46 +1.6 1,769,751 - ydiM b1690 0.54 +1.6 1,769,770 - ydiM b1690 0.56 +0.1 1,769,791 - ydiM b1690 0.57 +0.6 1,769,817 + ydiM b1690 0.59 +1.6 1,769,817 - ydiM b1690 0.59 +1.6 1,769,847 - ydiM b1690 0.62 +1.6 1,769,847 - ydiM b1690 0.62 +1.6 1,769,942 - ydiM b1690 0.70 +1.6 1,769,942 - ydiM b1690 0.70 -0.7 1,769,951 + ydiM b1690 0.70 +1.6 1,770,096 + ydiM b1690 0.82 +1.6 1,770,106 + ydiM b1690 0.83 +1.6 1,770,858 - ydiN b1691 0.25 +1.6 1,770,860 - ydiN b1691 0.26 +1.6 1,770,860 - ydiN b1691 0.26 +0.6 1,770,870 - ydiN b1691 0.26 +0.6 1,770,870 - ydiN b1691 0.26 +0.4 1,770,870 - ydiN b1691 0.26 +2.2 1,770,882 - ydiN b1691 0.27 -1.2 1,770,906 - ydiN b1691 0.29 +1.6 1,771,124 + ydiN b1691 0.46 +2.6 1,771,160 + ydiN b1691 0.49 +1.6 1,771,195 + ydiN b1691 0.52 -0.9 1,771,269 + ydiN b1691 0.58 +0.6 1,771,273 + ydiN b1691 0.58 -0.7 1,771,273 + ydiN b1691 0.58 +2.0 1,771,273 + ydiN b1691 0.58 -0.9
Or see this region's nucleotide sequence