Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tfaQ and stfQ overlap by 1 nucleotides stfQ and nohA overlap by 8 nucleotides nohA and ynfO are separated by 388 nucleotides ynfO and ydfO are separated by 57 nucleotides
b1546: tfaQ - Qin prophage; predicted tail fibre assembly protein (NCBI), at 1,632,334 to 1,632,909
tfaQ
b1547: stfQ - Qin prophage; predicted side tail fibre assembly protein (NCBI), at 1,632,909 to 1,633,871
stfQ
b1548: nohA - Qin prophage; predicted packaging protein (RefSeq), at 1,633,864 to 1,634,391
nohA
b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013
ynfO
b1549: ydfO - orf, hypothetical protein (VIMSS), at 1,635,071 to 1,635,481
ydfO
Position (kb)
1633
1634
1635 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1632.995 kb on + strand at 1633.092 kb on - strand, within stfQ at 1633.234 kb on + strand, within stfQ at 1633.234 kb on + strand, within stfQ at 1633.282 kb on - strand, within stfQ at 1633.282 kb on - strand, within stfQ at 1633.282 kb on - strand, within stfQ at 1633.716 kb on + strand, within stfQ at 1633.724 kb on - strand, within stfQ at 1633.724 kb on - strand, within stfQ at 1633.829 kb on - strand at 1634.153 kb on + strand, within nohA at 1634.153 kb on + strand, within nohA at 1634.884 kb on + strand, within ynfO at 1634.884 kb on + strand, within ynfO at 1634.916 kb on + strand, within ynfO at 1634.916 kb on + strand, within ynfO
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,632,995 + +0.2 1,633,092 - stfQ b1547 0.19 +0.2 1,633,234 + stfQ b1547 0.34 +0.2 1,633,234 + stfQ b1547 0.34 +0.3 1,633,282 - stfQ b1547 0.39 +0.8 1,633,282 - stfQ b1547 0.39 -0.5 1,633,282 - stfQ b1547 0.39 +0.1 1,633,716 + stfQ b1547 0.84 +0.3 1,633,724 - stfQ b1547 0.85 -0.1 1,633,724 - stfQ b1547 0.85 -0.4 1,633,829 - -0.0 1,634,153 + nohA b1548 0.55 -0.3 1,634,153 + nohA b1548 0.55 -0.8 1,634,884 + ynfO b4533 0.44 +0.6 1,634,884 + ynfO b4533 0.44 -0.6 1,634,916 + ynfO b4533 0.58 +3.4 1,634,916 + ynfO b4533 0.58 +0.8
Or see this region's nucleotide sequence