Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeT and yneL are separated by 254 nucleotides yneL and hipA are separated by 317 nucleotides hipA and hipB overlap by 1 nucleotides hipB and yneO are separated by 222 nucleotides
b1505: ydeT - hypothetical protein (RefSeq), at 1,586,877 to 1,588,103
ydeT
b1506: yneL - predicted transcriptional regulator (NCBI), at 1,588,358 to 1,588,560
yneL
b1507: hipA - regulator with hipB (NCBI), at 1,588,878 to 1,590,200
hipA
b1508: hipB - DNA-binding transcriptional regulator (NCBI), at 1,590,200 to 1,590,466
hipB
b4696: yneO - no description, at 1,590,689 to 1,596,011
yneO
Position (kb)
1588
1589
1590
1591 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1588.339 kb on - strand at 1588.354 kb on - strand at 1588.406 kb on - strand, within yneL at 1588.460 kb on - strand, within yneL at 1588.474 kb on - strand, within yneL at 1588.483 kb on - strand, within yneL at 1588.483 kb on - strand, within yneL at 1588.494 kb on - strand, within yneL at 1588.997 kb on - strand at 1589.129 kb on + strand, within hipA at 1589.163 kb on - strand, within hipA at 1589.222 kb on + strand, within hipA at 1589.337 kb on - strand, within hipA at 1589.584 kb on + strand, within hipA at 1589.584 kb on + strand, within hipA at 1589.592 kb on - strand, within hipA at 1589.592 kb on - strand, within hipA at 1589.629 kb on - strand, within hipA at 1589.888 kb on - strand, within hipA at 1589.977 kb on + strand, within hipA at 1590.648 kb on + strand at 1590.772 kb on + strand at 1590.807 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,588,339 - +1.4 1,588,354 - +0.0 1,588,406 - yneL b1506 0.24 -0.2 1,588,460 - yneL b1506 0.50 +0.6 1,588,474 - yneL b1506 0.57 -0.4 1,588,483 - yneL b1506 0.62 +0.0 1,588,483 - yneL b1506 0.62 -1.2 1,588,494 - yneL b1506 0.67 +1.0 1,588,997 - -0.7 1,589,129 + hipA b1507 0.19 +2.6 1,589,163 - hipA b1507 0.22 +1.2 1,589,222 + hipA b1507 0.26 +3.2 1,589,337 - hipA b1507 0.35 +0.7 1,589,584 + hipA b1507 0.53 -1.1 1,589,584 + hipA b1507 0.53 +0.7 1,589,592 - hipA b1507 0.54 +1.0 1,589,592 - hipA b1507 0.54 +1.2 1,589,629 - hipA b1507 0.57 +0.8 1,589,888 - hipA b1507 0.76 +1.0 1,589,977 + hipA b1507 0.83 -0.8 1,590,648 + +1.1 1,590,772 + -0.6 1,590,807 + +0.0
Or see this region's nucleotide sequence