Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gadB and pqqL are separated by 361 nucleotides pqqL and yddB are separated by 44 nucleotides
b1493: gadB - glutamate decarboxylase B, PLP-dependent (NCBI), at 1,568,669 to 1,570,069
gadB
b1494: pqqL - predicted peptidase (NCBI), at 1,570,431 to 1,573,226
pqqL
b1495: yddB - predicted porin protein (NCBI), at 1,573,271 to 1,575,643
yddB
Position (kb)
1570
1571
1572
1573
1574 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1569.776 kb on + strand, within gadB at 1570.028 kb on + strand at 1570.028 kb on + strand at 1570.123 kb on + strand at 1570.123 kb on + strand at 1570.423 kb on - strand at 1570.503 kb on - strand at 1570.503 kb on - strand at 1570.513 kb on - strand at 1570.541 kb on - strand at 1570.541 kb on - strand at 1570.541 kb on - strand at 1570.541 kb on - strand at 1570.546 kb on - strand at 1570.550 kb on - strand at 1570.551 kb on - strand at 1570.551 kb on - strand at 1570.551 kb on - strand at 1570.551 kb on - strand at 1570.551 kb on - strand at 1570.568 kb on - strand at 1570.568 kb on - strand at 1570.568 kb on - strand at 1570.588 kb on - strand at 1570.624 kb on - strand at 1570.624 kb on - strand at 1570.664 kb on + strand at 1570.714 kb on + strand, within pqqL at 1570.787 kb on + strand, within pqqL at 1570.817 kb on + strand, within pqqL at 1570.827 kb on + strand, within pqqL at 1570.827 kb on + strand, within pqqL at 1570.989 kb on + strand, within pqqL at 1571.004 kb on + strand, within pqqL at 1571.178 kb on - strand, within pqqL at 1571.207 kb on - strand, within pqqL at 1571.216 kb on + strand, within pqqL at 1571.224 kb on - strand, within pqqL at 1571.225 kb on + strand, within pqqL at 1571.268 kb on - strand, within pqqL at 1571.273 kb on - strand, within pqqL at 1571.469 kb on - strand, within pqqL at 1571.482 kb on - strand, within pqqL at 1571.617 kb on + strand, within pqqL at 1571.640 kb on - strand, within pqqL at 1571.641 kb on + strand, within pqqL at 1571.711 kb on + strand, within pqqL at 1571.756 kb on - strand, within pqqL at 1571.778 kb on + strand, within pqqL at 1571.900 kb on + strand, within pqqL at 1571.917 kb on - strand, within pqqL at 1572.021 kb on - strand, within pqqL at 1572.065 kb on - strand, within pqqL at 1572.125 kb on + strand, within pqqL at 1572.130 kb on + strand, within pqqL at 1572.130 kb on + strand, within pqqL at 1572.244 kb on + strand, within pqqL at 1572.286 kb on - strand, within pqqL at 1572.507 kb on + strand, within pqqL at 1572.546 kb on + strand, within pqqL at 1572.619 kb on + strand, within pqqL at 1572.619 kb on + strand, within pqqL at 1572.686 kb on + strand, within pqqL at 1572.789 kb on - strand, within pqqL at 1572.789 kb on - strand, within pqqL at 1572.792 kb on - strand, within pqqL at 1572.876 kb on + strand, within pqqL at 1572.877 kb on + strand, within pqqL at 1572.882 kb on + strand, within pqqL at 1572.882 kb on + strand, within pqqL at 1572.944 kb on - strand, within pqqL at 1573.008 kb on - strand at 1573.066 kb on - strand at 1573.066 kb on - strand at 1573.074 kb on - strand at 1573.074 kb on - strand at 1573.084 kb on - strand at 1573.193 kb on + strand at 1573.278 kb on - strand at 1573.297 kb on + strand at 1573.311 kb on + strand at 1573.322 kb on - strand at 1573.361 kb on + strand at 1573.439 kb on + strand at 1573.445 kb on + strand at 1573.445 kb on + strand at 1573.566 kb on + strand, within yddB at 1573.576 kb on + strand, within yddB at 1573.595 kb on + strand, within yddB at 1573.595 kb on + strand, within yddB at 1573.622 kb on + strand, within yddB at 1573.727 kb on + strand, within yddB at 1573.748 kb on + strand, within yddB at 1573.753 kb on + strand, within yddB at 1573.824 kb on - strand, within yddB at 1573.860 kb on + strand, within yddB at 1573.954 kb on - strand at 1573.965 kb on - strand, within yddB at 1573.990 kb on - strand, within yddB at 1574.031 kb on - strand, within yddB at 1574.031 kb on - strand, within yddB at 1574.063 kb on - strand, within yddB at 1574.072 kb on + strand, within yddB at 1574.074 kb on - strand, within yddB
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,569,776 + gadB b1493 0.79 -1.5 1,570,028 + -1.5 1,570,028 + +1.6 1,570,123 + +0.0 1,570,123 + +0.4 1,570,423 - +1.6 1,570,503 - -0.0 1,570,503 - +0.0 1,570,513 - +0.6 1,570,541 - +0.4 1,570,541 - +0.1 1,570,541 - -0.2 1,570,541 - +0.5 1,570,546 - +0.9 1,570,550 - -0.1 1,570,551 - +0.0 1,570,551 - -0.1 1,570,551 - +0.0 1,570,551 - +0.5 1,570,551 - -0.2 1,570,568 - +1.1 1,570,568 - +0.4 1,570,568 - +0.7 1,570,588 - +0.4 1,570,624 - +0.5 1,570,624 - +0.7 1,570,664 + +1.6 1,570,714 + pqqL b1494 0.10 +0.5 1,570,787 + pqqL b1494 0.13 +1.7 1,570,817 + pqqL b1494 0.14 -0.5 1,570,827 + pqqL b1494 0.14 +1.2 1,570,827 + pqqL b1494 0.14 +0.5 1,570,989 + pqqL b1494 0.20 +1.4 1,571,004 + pqqL b1494 0.20 +0.3 1,571,178 - pqqL b1494 0.27 +0.1 1,571,207 - pqqL b1494 0.28 -1.3 1,571,216 + pqqL b1494 0.28 +1.0 1,571,224 - pqqL b1494 0.28 +1.0 1,571,225 + pqqL b1494 0.28 +0.6 1,571,268 - pqqL b1494 0.30 -0.4 1,571,273 - pqqL b1494 0.30 +1.2 1,571,469 - pqqL b1494 0.37 +0.6 1,571,482 - pqqL b1494 0.38 +3.0 1,571,617 + pqqL b1494 0.42 +0.0 1,571,640 - pqqL b1494 0.43 +0.6 1,571,641 + pqqL b1494 0.43 +1.6 1,571,711 + pqqL b1494 0.46 -0.1 1,571,756 - pqqL b1494 0.47 +0.5 1,571,778 + pqqL b1494 0.48 +0.9 1,571,900 + pqqL b1494 0.53 +0.6 1,571,917 - pqqL b1494 0.53 +0.1 1,572,021 - pqqL b1494 0.57 +0.7 1,572,065 - pqqL b1494 0.58 -2.0 1,572,125 + pqqL b1494 0.61 -1.3 1,572,130 + pqqL b1494 0.61 +0.0 1,572,130 + pqqL b1494 0.61 +1.3 1,572,244 + pqqL b1494 0.65 +0.9 1,572,286 - pqqL b1494 0.66 -0.4 1,572,507 + pqqL b1494 0.74 +0.8 1,572,546 + pqqL b1494 0.76 +0.0 1,572,619 + pqqL b1494 0.78 +0.7 1,572,619 + pqqL b1494 0.78 -0.2 1,572,686 + pqqL b1494 0.81 -1.0 1,572,789 - pqqL b1494 0.84 +0.2 1,572,789 - pqqL b1494 0.84 -2.8 1,572,792 - pqqL b1494 0.84 -1.0 1,572,876 + pqqL b1494 0.87 +0.0 1,572,877 + pqqL b1494 0.87 +0.7 1,572,882 + pqqL b1494 0.88 +1.4 1,572,882 + pqqL b1494 0.88 -0.5 1,572,944 - pqqL b1494 0.90 -2.7 1,573,008 - -0.3 1,573,066 - +0.3 1,573,066 - +0.2 1,573,074 - +0.9 1,573,074 - +0.9 1,573,084 - +0.9 1,573,193 + -1.4 1,573,278 - +1.0 1,573,297 + -0.8 1,573,311 + +0.4 1,573,322 - +0.7 1,573,361 + -0.2 1,573,439 + -0.4 1,573,445 + +0.0 1,573,445 + -0.4 1,573,566 + yddB b1495 0.12 -0.6 1,573,576 + yddB b1495 0.13 +0.8 1,573,595 + yddB b1495 0.14 -0.1 1,573,595 + yddB b1495 0.14 +0.7 1,573,622 + yddB b1495 0.15 -0.5 1,573,727 + yddB b1495 0.19 +0.0 1,573,748 + yddB b1495 0.20 +1.6 1,573,753 + yddB b1495 0.20 -0.5 1,573,824 - yddB b1495 0.23 +0.7 1,573,860 + yddB b1495 0.25 -1.7 1,573,954 - -0.5 1,573,965 - yddB b1495 0.29 +0.8 1,573,990 - yddB b1495 0.30 -0.3 1,574,031 - yddB b1495 0.32 -1.5 1,574,031 - yddB b1495 0.32 +0.2 1,574,063 - yddB b1495 0.33 -1.3 1,574,072 + yddB b1495 0.34 +1.6 1,574,074 - yddB b1495 0.34 -2.1
Or see this region's nucleotide sequence