Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdnI and yddM are separated by 406 nucleotides yddM and adhP are separated by 145 nucleotides adhP and sfcA are separated by 133 nucleotides
b1476: fdnI - formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI), at 1,549,362 to 1,550,015
fdnI
b1477: yddM - predicted DNA-binding transcriptional regulator (RefSeq), at 1,550,422 to 1,550,706
yddM
b1478: adhP - alcohol dehydrogenase (VIMSS), at 1,550,852 to 1,551,862
adhP
b1479: sfcA - NAD-linked malate dehydrogenase (malic enzyme) (VIMSS), at 1,551,996 to 1,553,693
sfcA
Position (kb)
1550
1551
1552 Strain fitness (log2 ratio)
-2
-1
0
1 at 1550.056 kb on - strand at 1550.189 kb on - strand at 1550.189 kb on - strand at 1550.209 kb on - strand at 1550.806 kb on + strand at 1550.833 kb on - strand at 1551.011 kb on + strand, within adhP at 1551.036 kb on - strand, within adhP at 1551.421 kb on + strand, within adhP at 1551.752 kb on + strand, within adhP at 1551.859 kb on + strand at 1551.859 kb on + strand at 1551.871 kb on - strand at 1552.077 kb on + strand at 1552.077 kb on + strand at 1552.093 kb on + strand at 1552.254 kb on - strand, within sfcA at 1552.274 kb on - strand, within sfcA at 1552.501 kb on + strand, within sfcA at 1552.509 kb on - strand, within sfcA at 1552.509 kb on - strand, within sfcA at 1552.638 kb on - strand, within sfcA at 1552.638 kb on - strand, within sfcA
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,550,056 - +0.2 1,550,189 - -0.1 1,550,189 - -0.4 1,550,209 - -0.1 1,550,806 + +0.3 1,550,833 - +0.1 1,551,011 + adhP b1478 0.16 +0.4 1,551,036 - adhP b1478 0.18 +1.1 1,551,421 + adhP b1478 0.56 +0.1 1,551,752 + adhP b1478 0.89 +0.2 1,551,859 + -0.4 1,551,859 + +0.3 1,551,871 - -0.5 1,552,077 + -0.6 1,552,077 + +0.8 1,552,093 + -0.0 1,552,254 - sfcA b1479 0.15 +0.2 1,552,274 - sfcA b1479 0.16 -0.4 1,552,501 + sfcA b1479 0.30 +0.5 1,552,509 - sfcA b1479 0.30 +0.3 1,552,509 - sfcA b1479 0.30 -2.1 1,552,638 - sfcA b1479 0.38 +0.5 1,552,638 - sfcA b1479 0.38 +1.0
Or see this region's nucleotide sequence