Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydbD and b1408 are separated by 170 nucleotides b1408 and ynbB overlap by 1 nucleotides ynbB and ynbC are separated by 15 nucleotides
b1407: ydbD - orf, hypothetical protein (VIMSS), at 1,473,168 to 1,475,474
ydbD
b1408: b1408 - probable enzyme (VIMSS), at 1,475,645 to 1,476,250
b1408
b1409: ynbB - predicted CDP-diglyceride synthase (NCBI), at 1,476,250 to 1,477,146
ynbB
b1410: ynbC - predicted hydrolase (NCBI), at 1,477,162 to 1,478,919
ynbC
Position (kb)
1476
1477
1478 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1475.254 kb on + strand at 1475.348 kb on + strand at 1475.383 kb on - strand at 1475.610 kb on - strand at 1475.691 kb on - strand at 1475.719 kb on + strand, within b1408 at 1475.771 kb on + strand, within b1408 at 1475.771 kb on + strand, within b1408 at 1475.790 kb on + strand, within b1408 at 1475.838 kb on + strand, within b1408 at 1475.838 kb on + strand, within b1408 at 1475.850 kb on + strand, within b1408 at 1475.850 kb on + strand, within b1408 at 1475.862 kb on + strand, within b1408 at 1475.862 kb on + strand, within b1408 at 1475.866 kb on + strand, within b1408 at 1475.866 kb on + strand, within b1408 at 1475.866 kb on - strand, within b1408 at 1475.903 kb on + strand, within b1408 at 1476.147 kb on + strand, within b1408 at 1476.147 kb on + strand, within b1408 at 1476.882 kb on - strand, within ynbB at 1476.882 kb on - strand, within ynbB at 1476.894 kb on - strand, within ynbB at 1476.902 kb on + strand, within ynbB at 1476.924 kb on + strand, within ynbB at 1476.934 kb on + strand, within ynbB at 1476.934 kb on + strand, within ynbB at 1477.000 kb on + strand, within ynbB at 1477.008 kb on + strand, within ynbB at 1477.055 kb on + strand, within ynbB at 1477.064 kb on - strand at 1477.066 kb on + strand at 1477.074 kb on + strand at 1477.088 kb on + strand at 1477.214 kb on - strand at 1477.256 kb on - strand at 1477.265 kb on - strand at 1477.317 kb on - strand at 1477.333 kb on - strand at 1477.397 kb on + strand, within ynbC at 1477.401 kb on - strand, within ynbC at 1477.563 kb on - strand, within ynbC at 1477.563 kb on - strand, within ynbC at 1477.624 kb on - strand, within ynbC at 1477.688 kb on - strand, within ynbC at 1477.781 kb on - strand, within ynbC at 1477.859 kb on - strand, within ynbC at 1478.058 kb on + strand, within ynbC
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,475,254 + -0.2 1,475,348 + -0.5 1,475,383 - +1.3 1,475,610 - +0.4 1,475,691 - +2.4 1,475,719 + b1408 b1408 0.12 -0.9 1,475,771 + b1408 b1408 0.21 -1.2 1,475,771 + b1408 b1408 0.21 -0.6 1,475,790 + b1408 b1408 0.24 -0.4 1,475,838 + b1408 b1408 0.32 -0.2 1,475,838 + b1408 b1408 0.32 -0.8 1,475,850 + b1408 b1408 0.34 -1.3 1,475,850 + b1408 b1408 0.34 +0.9 1,475,862 + b1408 b1408 0.36 -0.7 1,475,862 + b1408 b1408 0.36 +1.1 1,475,866 + b1408 b1408 0.36 +0.3 1,475,866 + b1408 b1408 0.36 +0.3 1,475,866 - b1408 b1408 0.36 +1.7 1,475,903 + b1408 b1408 0.43 +1.5 1,476,147 + b1408 b1408 0.83 +0.7 1,476,147 + b1408 b1408 0.83 -0.3 1,476,882 - ynbB b1409 0.70 +1.7 1,476,882 - ynbB b1409 0.70 +1.7 1,476,894 - ynbB b1409 0.72 +1.7 1,476,902 + ynbB b1409 0.73 -0.6 1,476,924 + ynbB b1409 0.75 +1.4 1,476,934 + ynbB b1409 0.76 +1.7 1,476,934 + ynbB b1409 0.76 +0.7 1,477,000 + ynbB b1409 0.84 +1.7 1,477,008 + ynbB b1409 0.85 -1.5 1,477,055 + ynbB b1409 0.90 +2.7 1,477,064 - +1.7 1,477,066 + +1.7 1,477,074 + +0.7 1,477,088 + +0.7 1,477,214 - +0.1 1,477,256 - +0.1 1,477,265 - +0.7 1,477,317 - +0.2 1,477,333 - -0.2 1,477,397 + ynbC b1410 0.13 -1.9 1,477,401 - ynbC b1410 0.14 +0.2 1,477,563 - ynbC b1410 0.23 +0.0 1,477,563 - ynbC b1410 0.23 +0.2 1,477,624 - ynbC b1410 0.26 -1.5 1,477,688 - ynbC b1410 0.30 +0.9 1,477,781 - ynbC b1410 0.35 -0.9 1,477,859 - ynbC b1410 0.40 -1.8 1,478,058 + ynbC b1410 0.51 +0.6
Or see this region's nucleotide sequence