Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjF and tyrR are separated by 147 nucleotides tyrR and tpx are separated by 43 nucleotides tpx and ycjG are separated by 118 nucleotides
b1322: ycjF - hypothetical protein (NCBI), at 1,383,535 to 1,384,596
ycjF
b1323: tyrR - DNA-binding transcriptional dual regulator, tyrosine-binding (NCBI), at 1,384,744 to 1,386,285
tyrR
b1324: tpx - thiol peroxidase (NCBI), at 1,386,329 to 1,386,835
tpx
b1325: ycjG - putative muconate cycloisomerase I (EC 5.5.-.-) (VIMSS), at 1,386,954 to 1,387,919
ycjG
Position (kb)
1384
1385
1386
1387 Strain fitness (log2 ratio)
-1
0
1
2 at 1383.781 kb on + strand, within ycjF at 1383.801 kb on - strand, within ycjF at 1383.801 kb on - strand, within ycjF at 1383.828 kb on - strand, within ycjF at 1383.892 kb on + strand, within ycjF at 1383.892 kb on + strand, within ycjF at 1383.937 kb on - strand, within ycjF at 1384.032 kb on + strand, within ycjF at 1384.032 kb on + strand, within ycjF at 1384.214 kb on + strand, within ycjF at 1384.214 kb on + strand, within ycjF at 1384.420 kb on + strand, within ycjF at 1384.428 kb on - strand, within ycjF at 1384.428 kb on - strand, within ycjF at 1384.428 kb on - strand, within ycjF at 1384.941 kb on + strand, within tyrR at 1384.949 kb on - strand, within tyrR at 1385.018 kb on - strand, within tyrR at 1385.154 kb on + strand, within tyrR at 1385.222 kb on + strand, within tyrR at 1385.222 kb on + strand, within tyrR at 1385.791 kb on + strand, within tyrR at 1385.987 kb on - strand, within tyrR at 1386.014 kb on + strand, within tyrR at 1386.352 kb on - strand at 1386.352 kb on - strand at 1386.442 kb on + strand, within tpx at 1386.528 kb on + strand, within tpx at 1386.585 kb on + strand at 1386.585 kb on + strand, within tpx at 1386.603 kb on - strand, within tpx at 1386.603 kb on - strand, within tpx at 1386.742 kb on - strand, within tpx at 1386.748 kb on + strand, within tpx at 1386.923 kb on + strand at 1387.059 kb on + strand, within ycjG at 1387.076 kb on - strand, within ycjG
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,383,781 + ycjF b1322 0.23 +0.1 1,383,801 - ycjF b1322 0.25 -0.3 1,383,801 - ycjF b1322 0.25 -0.6 1,383,828 - ycjF b1322 0.28 +0.6 1,383,892 + ycjF b1322 0.34 -0.9 1,383,892 + ycjF b1322 0.34 -1.0 1,383,937 - ycjF b1322 0.38 +0.1 1,384,032 + ycjF b1322 0.47 +0.1 1,384,032 + ycjF b1322 0.47 +0.1 1,384,214 + ycjF b1322 0.64 +0.2 1,384,214 + ycjF b1322 0.64 -0.4 1,384,420 + ycjF b1322 0.83 +0.3 1,384,428 - ycjF b1322 0.84 +1.4 1,384,428 - ycjF b1322 0.84 +0.1 1,384,428 - ycjF b1322 0.84 -0.3 1,384,941 + tyrR b1323 0.13 +0.6 1,384,949 - tyrR b1323 0.13 +0.5 1,385,018 - tyrR b1323 0.18 +0.2 1,385,154 + tyrR b1323 0.27 +0.9 1,385,222 + tyrR b1323 0.31 +0.3 1,385,222 + tyrR b1323 0.31 +0.8 1,385,791 + tyrR b1323 0.68 -0.4 1,385,987 - tyrR b1323 0.81 +0.6 1,386,014 + tyrR b1323 0.82 +0.7 1,386,352 - -0.4 1,386,352 - +0.2 1,386,442 + tpx b1324 0.22 +0.3 1,386,528 + tpx b1324 0.39 -0.5 1,386,585 + +2.1 1,386,585 + tpx b1324 0.50 +2.7 1,386,603 - tpx b1324 0.54 +0.3 1,386,603 - tpx b1324 0.54 -0.3 1,386,742 - tpx b1324 0.81 -0.4 1,386,748 + tpx b1324 0.83 +0.4 1,386,923 + -0.3 1,387,059 + ycjG b1325 0.11 +0.9 1,387,076 - ycjG b1325 0.13 +0.4
Or see this region's nucleotide sequence