Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapA and ymjA are separated by 312 nucleotides ymjA and ycjJ are separated by 133 nucleotides ycjJ and b1297 are separated by 302 nucleotides
b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134
sapA
b1295: ymjA - hypothetical protein (NCBI), at 1,355,447 to 1,355,692
ymjA
b1296: ycjJ - putative amino acid/amine transport protein (VIMSS), at 1,355,826 to 1,357,211
ycjJ
b1297: b1297 - putative glutamine synthetase (EC 6.3.1.2) (VIMSS), at 1,357,514 to 1,358,932
b1297
Position (kb)
1355
1356
1357
1358 Strain fitness (log2 ratio)
-1
0
1
2 at 1354.915 kb on - strand, within sapA at 1354.915 kb on - strand, within sapA at 1354.998 kb on - strand at 1355.215 kb on + strand at 1355.225 kb on - strand at 1355.225 kb on - strand at 1355.370 kb on - strand at 1355.370 kb on - strand at 1355.445 kb on - strand at 1355.485 kb on + strand, within ymjA at 1355.488 kb on - strand, within ymjA at 1355.488 kb on - strand, within ymjA at 1355.507 kb on - strand, within ymjA at 1355.713 kb on - strand at 1355.723 kb on - strand at 1355.726 kb on - strand at 1355.728 kb on - strand at 1355.853 kb on - strand at 1355.862 kb on + strand at 1356.256 kb on + strand, within ycjJ at 1356.736 kb on - strand, within ycjJ at 1356.934 kb on - strand, within ycjJ at 1356.934 kb on - strand, within ycjJ at 1356.948 kb on + strand, within ycjJ at 1356.948 kb on + strand, within ycjJ at 1356.949 kb on + strand, within ycjJ at 1356.949 kb on + strand, within ycjJ at 1357.116 kb on - strand at 1357.116 kb on - strand at 1357.150 kb on + strand at 1357.189 kb on - strand at 1357.489 kb on - strand at 1357.696 kb on - strand, within b1297 at 1357.696 kb on - strand, within b1297 at 1357.820 kb on + strand, within b1297 at 1357.845 kb on - strand, within b1297 at 1357.845 kb on - strand, within b1297 at 1357.898 kb on - strand, within b1297
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,354,915 - sapA b1294 0.87 +0.9 1,354,915 - sapA b1294 0.87 -0.4 1,354,998 - -0.4 1,355,215 + -0.0 1,355,225 - -0.8 1,355,225 - -0.0 1,355,370 - -0.8 1,355,370 - -0.6 1,355,445 - -0.6 1,355,485 + ymjA b1295 0.15 -0.2 1,355,488 - ymjA b1295 0.17 +1.9 1,355,488 - ymjA b1295 0.17 -0.4 1,355,507 - ymjA b1295 0.24 -0.6 1,355,713 - -0.1 1,355,723 - +0.3 1,355,726 - -0.7 1,355,728 - +0.2 1,355,853 - +0.8 1,355,862 + +0.5 1,356,256 + ycjJ b1296 0.31 -0.1 1,356,736 - ycjJ b1296 0.66 +0.2 1,356,934 - ycjJ b1296 0.80 -0.8 1,356,934 - ycjJ b1296 0.80 +1.7 1,356,948 + ycjJ b1296 0.81 +1.5 1,356,948 + ycjJ b1296 0.81 -0.1 1,356,949 + ycjJ b1296 0.81 +0.8 1,356,949 + ycjJ b1296 0.81 +0.2 1,357,116 - +0.8 1,357,116 - -0.3 1,357,150 + +0.3 1,357,189 - +0.2 1,357,489 - -0.3 1,357,696 - b1297 b1297 0.13 -0.2 1,357,696 - b1297 b1297 0.13 +1.5 1,357,820 + b1297 b1297 0.22 -0.6 1,357,845 - b1297 b1297 0.23 -0.5 1,357,845 - b1297 b1297 0.23 +0.7 1,357,898 - b1297 b1297 0.27 -0.2
Or see this region's nucleotide sequence