Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chaC and ychN are separated by 43 nucleotides ychN and ychO are separated by 184 nucleotides ychO and narL are separated by 0 nucleotides narL and narX overlap by 8 nucleotides
b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425
chaC
b1219: ychN - hypothetical protein (NCBI), at 1,272,469 to 1,272,822
ychN
b1220: ychO - predicted invasin (RefSeq), at 1,273,007 to 1,274,401
ychO
b1221: narL - DNA-binding response regulator in two-component regulatory system with NarX (or NarQ) (NCBI), at 1,274,402 to 1,275,052
narL
b1222: narX - sensory histidine kinase in two-component regulatory system with NarL (NCBI), at 1,275,045 to 1,276,841
narX
Position (kb)
1273
1274
1275 Strain fitness (log2 ratio)
-1
0
1 at 1272.143 kb on - strand, within chaC at 1272.159 kb on - strand, within chaC at 1272.288 kb on - strand, within chaC at 1272.522 kb on - strand, within ychN at 1272.532 kb on - strand, within ychN at 1272.581 kb on - strand, within ychN at 1272.615 kb on - strand, within ychN at 1272.714 kb on + strand, within ychN at 1272.787 kb on - strand at 1272.787 kb on - strand at 1273.039 kb on + strand at 1273.305 kb on + strand, within ychO at 1273.328 kb on - strand, within ychO at 1273.328 kb on - strand, within ychO at 1273.463 kb on + strand, within ychO at 1273.493 kb on + strand, within ychO at 1273.732 kb on + strand, within ychO at 1273.737 kb on + strand, within ychO at 1273.930 kb on - strand, within ychO at 1274.003 kb on + strand, within ychO at 1274.343 kb on - strand at 1274.391 kb on + strand at 1274.489 kb on - strand, within narL at 1274.543 kb on + strand, within narL at 1274.543 kb on + strand, within narL at 1274.583 kb on - strand, within narL at 1274.668 kb on - strand, within narL at 1274.677 kb on - strand, within narL at 1274.677 kb on - strand, within narL at 1275.022 kb on + strand at 1275.022 kb on + strand at 1275.035 kb on - strand at 1275.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,272,143 - chaC b1218 0.59 -0.2 1,272,159 - chaC b1218 0.62 +0.4 1,272,288 - chaC b1218 0.80 -0.0 1,272,522 - ychN b1219 0.15 +0.9 1,272,532 - ychN b1219 0.18 -0.2 1,272,581 - ychN b1219 0.32 +0.1 1,272,615 - ychN b1219 0.41 +0.4 1,272,714 + ychN b1219 0.69 -0.9 1,272,787 - +0.0 1,272,787 - +0.1 1,273,039 + -0.6 1,273,305 + ychO b1220 0.21 +1.1 1,273,328 - ychO b1220 0.23 +0.1 1,273,328 - ychO b1220 0.23 -0.7 1,273,463 + ychO b1220 0.33 +1.2 1,273,493 + ychO b1220 0.35 +1.3 1,273,732 + ychO b1220 0.52 +0.8 1,273,737 + ychO b1220 0.52 -0.1 1,273,930 - ychO b1220 0.66 +1.4 1,274,003 + ychO b1220 0.71 +1.2 1,274,343 - +0.5 1,274,391 + -0.1 1,274,489 - narL b1221 0.13 +0.5 1,274,543 + narL b1221 0.22 -0.8 1,274,543 + narL b1221 0.22 -0.1 1,274,583 - narL b1221 0.28 +0.0 1,274,668 - narL b1221 0.41 +0.2 1,274,677 - narL b1221 0.42 +0.3 1,274,677 - narL b1221 0.42 +0.7 1,275,022 + -1.1 1,275,022 + -0.3 1,275,035 - +1.0 1,275,035 - -0.3
Or see this region's nucleotide sequence