Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymgE and ycgY are separated by 177 nucleotides ycgY and treA are separated by 78 nucleotides treA and dhaM are separated by 319 nucleotides
b1195: ymgE - predicted inner membrane protein (NCBI), at 1,243,951 to 1,244,205
ymgE
b1196: ycgY - hypothetical protein (NCBI), at 1,244,383 to 1,244,823
ycgY
b1197: treA - periplasmic trehalase (NCBI), at 1,244,902 to 1,246,599
treA
b1198: dhaM - fused predicted dihydroxyacetone-specific PTS enzymes: HPr component/EI component (RefSeq), at 1,246,919 to 1,248,337
dhaM
Position (kb)
1244
1245
1246
1247 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1243.904 kb on + strand at 1244.024 kb on - strand, within ymgE at 1244.060 kb on - strand, within ymgE at 1244.118 kb on + strand, within ymgE at 1244.160 kb on + strand, within ymgE at 1244.160 kb on + strand, within ymgE at 1244.164 kb on + strand, within ymgE at 1244.225 kb on + strand at 1244.225 kb on + strand at 1244.225 kb on + strand at 1244.238 kb on + strand at 1244.262 kb on + strand at 1244.267 kb on + strand at 1244.328 kb on + strand at 1244.328 kb on + strand at 1244.328 kb on + strand at 1244.336 kb on - strand at 1244.336 kb on - strand at 1244.417 kb on + strand at 1244.430 kb on - strand, within ycgY at 1244.451 kb on + strand, within ycgY at 1244.603 kb on - strand, within ycgY at 1244.645 kb on + strand, within ycgY at 1244.677 kb on - strand, within ycgY at 1244.681 kb on - strand, within ycgY at 1244.685 kb on + strand, within ycgY at 1244.685 kb on + strand, within ycgY at 1244.756 kb on + strand, within ycgY at 1244.760 kb on - strand, within ycgY at 1244.777 kb on + strand, within ycgY at 1244.911 kb on + strand at 1244.940 kb on - strand at 1245.040 kb on - strand at 1245.055 kb on + strand at 1245.144 kb on + strand, within treA at 1245.144 kb on + strand, within treA at 1245.150 kb on - strand, within treA at 1245.170 kb on + strand, within treA at 1245.237 kb on - strand, within treA at 1245.247 kb on - strand, within treA at 1245.292 kb on - strand, within treA at 1245.292 kb on - strand, within treA at 1245.297 kb on - strand, within treA at 1245.297 kb on - strand, within treA at 1245.375 kb on - strand, within treA at 1245.458 kb on + strand, within treA at 1245.458 kb on + strand, within treA at 1245.548 kb on + strand, within treA at 1245.877 kb on + strand, within treA at 1245.952 kb on + strand, within treA at 1245.952 kb on + strand, within treA at 1246.107 kb on - strand, within treA at 1246.158 kb on + strand, within treA at 1246.160 kb on + strand, within treA at 1246.160 kb on + strand, within treA at 1246.168 kb on - strand, within treA at 1246.185 kb on - strand, within treA at 1246.410 kb on - strand, within treA at 1246.505 kb on - strand at 1246.513 kb on - strand at 1246.666 kb on - strand at 1246.696 kb on - strand at 1246.799 kb on - strand at 1246.799 kb on - strand at 1246.898 kb on - strand at 1246.904 kb on + strand at 1246.924 kb on + strand at 1246.925 kb on - strand at 1246.944 kb on + strand at 1246.944 kb on + strand at 1247.303 kb on + strand, within dhaM at 1247.303 kb on + strand, within dhaM at 1247.349 kb on + strand, within dhaM at 1247.376 kb on + strand, within dhaM at 1247.377 kb on + strand, within dhaM at 1247.377 kb on - strand, within dhaM at 1247.497 kb on - strand, within dhaM at 1247.566 kb on - strand, within dhaM
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,243,904 + +0.1 1,244,024 - ymgE b1195 0.29 -0.5 1,244,060 - ymgE b1195 0.43 +0.5 1,244,118 + ymgE b1195 0.65 -0.8 1,244,160 + ymgE b1195 0.82 +0.1 1,244,160 + ymgE b1195 0.82 -1.1 1,244,164 + ymgE b1195 0.84 +0.5 1,244,225 + +0.4 1,244,225 + -0.2 1,244,225 + -0.5 1,244,238 + +0.0 1,244,262 + +0.3 1,244,267 + -0.3 1,244,328 + +1.5 1,244,328 + +0.2 1,244,328 + -0.5 1,244,336 - +0.2 1,244,336 - -0.2 1,244,417 + +0.0 1,244,430 - ycgY b1196 0.11 +2.6 1,244,451 + ycgY b1196 0.15 +1.6 1,244,603 - ycgY b1196 0.50 +1.8 1,244,645 + ycgY b1196 0.59 +1.8 1,244,677 - ycgY b1196 0.67 -0.3 1,244,681 - ycgY b1196 0.68 +1.8 1,244,685 + ycgY b1196 0.68 +0.8 1,244,685 + ycgY b1196 0.68 -0.9 1,244,756 + ycgY b1196 0.85 -2.3 1,244,760 - ycgY b1196 0.85 +0.8 1,244,777 + ycgY b1196 0.89 -1.2 1,244,911 + +1.2 1,244,940 - -0.6 1,245,040 - +0.1 1,245,055 + +1.8 1,245,144 + treA b1197 0.14 +0.2 1,245,144 + treA b1197 0.14 +0.2 1,245,150 - treA b1197 0.15 +0.2 1,245,170 + treA b1197 0.16 +0.3 1,245,237 - treA b1197 0.20 +0.6 1,245,247 - treA b1197 0.20 +0.8 1,245,292 - treA b1197 0.23 -0.3 1,245,292 - treA b1197 0.23 +0.3 1,245,297 - treA b1197 0.23 +1.0 1,245,297 - treA b1197 0.23 +0.3 1,245,375 - treA b1197 0.28 +0.9 1,245,458 + treA b1197 0.33 +0.5 1,245,458 + treA b1197 0.33 -1.0 1,245,548 + treA b1197 0.38 +0.1 1,245,877 + treA b1197 0.57 -0.6 1,245,952 + treA b1197 0.62 +0.2 1,245,952 + treA b1197 0.62 +0.4 1,246,107 - treA b1197 0.71 +1.4 1,246,158 + treA b1197 0.74 -0.3 1,246,160 + treA b1197 0.74 +0.2 1,246,160 + treA b1197 0.74 +0.8 1,246,168 - treA b1197 0.75 -0.7 1,246,185 - treA b1197 0.76 +1.7 1,246,410 - treA b1197 0.89 -1.2 1,246,505 - -0.0 1,246,513 - +0.8 1,246,666 - +0.3 1,246,696 - +0.8 1,246,799 - -0.5 1,246,799 - +1.3 1,246,898 - +1.2 1,246,904 + +0.8 1,246,924 + +0.2 1,246,925 - -0.5 1,246,944 + -0.6 1,246,944 + -0.5 1,247,303 + dhaM b1198 0.27 +1.6 1,247,303 + dhaM b1198 0.27 -1.0 1,247,349 + dhaM b1198 0.30 -0.6 1,247,376 + dhaM b1198 0.32 +1.8 1,247,377 + dhaM b1198 0.32 -0.0 1,247,377 - dhaM b1198 0.32 -0.2 1,247,497 - dhaM b1198 0.41 +0.1 1,247,566 - dhaM b1198 0.46 +0.8
Or see this region's nucleotide sequence