Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycfD and phoQ are separated by 75 nucleotides phoQ and phoP overlap by 1 nucleotides phoP and purB are separated by 168 nucleotides
b1128: ycfD - orf, hypothetical protein (VIMSS), at 1,186,342 to 1,187,463
ycfD
b1129: phoQ - sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI), at 1,187,539 to 1,188,999
phoQ
b1130: phoP - DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI), at 1,188,999 to 1,189,670
phoP
b1131: purB - adenylosuccinate lyase (NCBI), at 1,189,839 to 1,191,209
purB
Position (kb)
1187
1188
1189 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1186.778 kb on + strand, within ycfD at 1186.778 kb on + strand, within ycfD at 1186.805 kb on - strand, within ycfD at 1186.876 kb on - strand, within ycfD at 1186.942 kb on - strand, within ycfD at 1186.950 kb on + strand, within ycfD at 1186.950 kb on + strand, within ycfD at 1187.202 kb on + strand, within ycfD at 1187.292 kb on + strand, within ycfD at 1187.292 kb on + strand, within ycfD at 1187.349 kb on + strand, within ycfD at 1187.349 kb on + strand, within ycfD at 1187.446 kb on + strand at 1187.521 kb on + strand at 1187.672 kb on - strand at 1187.672 kb on - strand at 1187.674 kb on - strand at 1187.674 kb on - strand at 1187.685 kb on + strand, within phoQ at 1187.775 kb on - strand, within phoQ at 1187.776 kb on - strand, within phoQ at 1187.776 kb on - strand, within phoQ at 1187.779 kb on + strand, within phoQ at 1187.844 kb on - strand, within phoQ at 1187.955 kb on + strand, within phoQ at 1187.973 kb on - strand, within phoQ at 1188.100 kb on - strand, within phoQ at 1188.191 kb on + strand, within phoQ at 1188.246 kb on + strand, within phoQ at 1188.320 kb on + strand, within phoQ at 1188.342 kb on + strand, within phoQ at 1188.342 kb on + strand, within phoQ at 1188.435 kb on - strand, within phoQ at 1188.435 kb on - strand, within phoQ at 1188.461 kb on + strand, within phoQ at 1188.564 kb on + strand, within phoQ at 1188.760 kb on - strand, within phoQ at 1188.760 kb on - strand, within phoQ at 1188.911 kb on + strand at 1188.924 kb on + strand at 1189.023 kb on - strand at 1189.104 kb on + strand, within phoP at 1189.121 kb on + strand, within phoP at 1189.135 kb on - strand, within phoP at 1189.135 kb on - strand, within phoP at 1189.137 kb on + strand, within phoP at 1189.137 kb on + strand, within phoP at 1189.137 kb on + strand, within phoP at 1189.320 kb on - strand, within phoP at 1189.320 kb on - strand, within phoP at 1189.330 kb on - strand, within phoP at 1189.375 kb on - strand, within phoP at 1189.375 kb on - strand, within phoP at 1189.395 kb on + strand, within phoP at 1189.403 kb on - strand, within phoP at 1189.483 kb on + strand, within phoP at 1189.587 kb on + strand, within phoP at 1189.587 kb on + strand, within phoP at 1189.590 kb on + strand, within phoP at 1189.614 kb on - strand at 1189.713 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,186,778 + ycfD b1128 0.39 +0.1 1,186,778 + ycfD b1128 0.39 +1.0 1,186,805 - ycfD b1128 0.41 +0.3 1,186,876 - ycfD b1128 0.48 -0.2 1,186,942 - ycfD b1128 0.53 -0.1 1,186,950 + ycfD b1128 0.54 -0.3 1,186,950 + ycfD b1128 0.54 +0.4 1,187,202 + ycfD b1128 0.77 -0.1 1,187,292 + ycfD b1128 0.85 -0.1 1,187,292 + ycfD b1128 0.85 -0.9 1,187,349 + ycfD b1128 0.90 -1.1 1,187,349 + ycfD b1128 0.90 +0.3 1,187,446 + -0.1 1,187,521 + -0.0 1,187,672 - -0.9 1,187,672 - +1.9 1,187,674 - +1.3 1,187,674 - +1.9 1,187,685 + phoQ b1129 0.10 +1.9 1,187,775 - phoQ b1129 0.16 +1.9 1,187,776 - phoQ b1129 0.16 +1.9 1,187,776 - phoQ b1129 0.16 +1.9 1,187,779 + phoQ b1129 0.16 -0.1 1,187,844 - phoQ b1129 0.21 -0.1 1,187,955 + phoQ b1129 0.28 +1.9 1,187,973 - phoQ b1129 0.30 +2.9 1,188,100 - phoQ b1129 0.38 -1.5 1,188,191 + phoQ b1129 0.45 +1.9 1,188,246 + phoQ b1129 0.48 +2.9 1,188,320 + phoQ b1129 0.53 +0.3 1,188,342 + phoQ b1129 0.55 +1.9 1,188,342 + phoQ b1129 0.55 -1.1 1,188,435 - phoQ b1129 0.61 +1.9 1,188,435 - phoQ b1129 0.61 +0.9 1,188,461 + phoQ b1129 0.63 +1.9 1,188,564 + phoQ b1129 0.70 +0.9 1,188,760 - phoQ b1129 0.84 +1.9 1,188,760 - phoQ b1129 0.84 -0.9 1,188,911 + +1.9 1,188,924 + +0.9 1,189,023 - -0.1 1,189,104 + phoP b1130 0.16 +0.3 1,189,121 + phoP b1130 0.18 +1.3 1,189,135 - phoP b1130 0.20 +1.9 1,189,135 - phoP b1130 0.20 +0.9 1,189,137 + phoP b1130 0.21 +0.3 1,189,137 + phoP b1130 0.21 +0.3 1,189,137 + phoP b1130 0.21 -0.1 1,189,320 - phoP b1130 0.48 +0.9 1,189,320 - phoP b1130 0.48 +2.5 1,189,330 - phoP b1130 0.49 -0.7 1,189,375 - phoP b1130 0.56 +1.9 1,189,375 - phoP b1130 0.56 +1.9 1,189,395 + phoP b1130 0.59 +1.9 1,189,403 - phoP b1130 0.60 +1.9 1,189,483 + phoP b1130 0.72 +0.9 1,189,587 + phoP b1130 0.88 +0.3 1,189,587 + phoP b1130 0.88 +2.5 1,189,590 + phoP b1130 0.88 +0.3 1,189,614 - +1.9 1,189,713 + +0.9
Or see this region's nucleotide sequence