Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagK and cobB are separated by 126 nucleotides cobB and ycfZ are separated by 119 nucleotides ycfZ and ymfA overlap by 4 nucleotides ymfA and potD are separated by 57 nucleotides
b1119: nagK - N-acetyl-D-glucosamine kinase (NCBI), at 1,177,816 to 1,178,727
nagK
b1120: cobB - deacetylase of acs and cheY, regulates chemotaxis (NCBI), at 1,178,854 to 1,179,582
cobB
b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490
ycfZ
b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948
ymfA
b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052
potD
Position (kb)
1179
1180
1181 Strain fitness (log2 ratio)
-1
0
1
2 at 1178.708 kb on + strand at 1178.708 kb on + strand at 1178.766 kb on + strand at 1178.766 kb on + strand at 1178.875 kb on + strand at 1178.875 kb on + strand at 1178.880 kb on - strand at 1178.880 kb on - strand at 1179.036 kb on - strand, within cobB at 1179.142 kb on + strand, within cobB at 1179.161 kb on + strand, within cobB at 1179.161 kb on + strand, within cobB at 1179.195 kb on - strand, within cobB at 1179.319 kb on + strand, within cobB at 1179.396 kb on + strand, within cobB at 1179.413 kb on + strand, within cobB at 1179.413 kb on + strand, within cobB at 1179.421 kb on - strand, within cobB at 1179.526 kb on + strand at 1179.552 kb on - strand at 1179.584 kb on + strand at 1179.584 kb on + strand at 1179.584 kb on + strand at 1179.594 kb on + strand at 1179.758 kb on - strand at 1179.768 kb on - strand at 1179.785 kb on + strand, within ycfZ at 1179.833 kb on + strand, within ycfZ at 1179.837 kb on - strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.943 kb on + strand, within ycfZ at 1179.992 kb on + strand, within ycfZ at 1180.234 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.438 kb on + strand at 1180.457 kb on + strand at 1180.739 kb on - strand, within ymfA at 1180.755 kb on - strand, within ymfA at 1180.755 kb on - strand, within ymfA at 1180.763 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.777 kb on - strand, within ymfA at 1180.778 kb on - strand, within ymfA at 1180.796 kb on + strand, within ymfA at 1180.796 kb on + strand, within ymfA at 1180.799 kb on - strand, within ymfA at 1180.800 kb on - strand, within ymfA at 1180.803 kb on - strand, within ymfA at 1180.809 kb on + strand, within ymfA at 1180.813 kb on + strand, within ymfA at 1180.817 kb on - strand, within ymfA at 1180.834 kb on - strand, within ymfA at 1180.838 kb on - strand, within ymfA at 1180.854 kb on - strand, within ymfA at 1180.869 kb on - strand, within ymfA at 1180.881 kb on + strand, within ymfA at 1180.881 kb on + strand, within ymfA at 1180.890 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.894 kb on + strand, within ymfA at 1180.900 kb on + strand, within ymfA at 1180.900 kb on + strand, within ymfA at 1180.902 kb on - strand at 1180.908 kb on - strand at 1180.937 kb on + strand at 1180.945 kb on - strand at 1180.945 kb on - strand at 1180.953 kb on - strand at 1180.959 kb on - strand at 1180.988 kb on - strand at 1180.988 kb on - strand at 1180.989 kb on - strand at 1180.989 kb on - strand at 1181.036 kb on - strand at 1181.046 kb on - strand at 1181.101 kb on + strand at 1181.101 kb on + strand at 1181.170 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.280 kb on - strand, within potD at 1181.421 kb on + strand, within potD at 1181.480 kb on - strand, within potD
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,178,708 + +0.0 1,178,708 + +0.7 1,178,766 + -0.0 1,178,766 + -0.1 1,178,875 + +0.1 1,178,875 + +0.6 1,178,880 - +1.3 1,178,880 - -0.9 1,179,036 - cobB b1120 0.25 -0.0 1,179,142 + cobB b1120 0.40 +0.6 1,179,161 + cobB b1120 0.42 +0.9 1,179,161 + cobB b1120 0.42 +0.6 1,179,195 - cobB b1120 0.47 +0.6 1,179,319 + cobB b1120 0.64 +1.9 1,179,396 + cobB b1120 0.74 +0.2 1,179,413 + cobB b1120 0.77 +0.6 1,179,413 + cobB b1120 0.77 +0.2 1,179,421 - cobB b1120 0.78 -0.4 1,179,526 + -1.4 1,179,552 - +0.3 1,179,584 + +0.3 1,179,584 + -0.3 1,179,584 + +0.3 1,179,594 + +0.3 1,179,758 - +1.1 1,179,768 - +0.3 1,179,785 + ycfZ b1121 0.11 -1.4 1,179,833 + ycfZ b1121 0.17 +0.4 1,179,837 - ycfZ b1121 0.17 +0.9 1,179,922 + ycfZ b1121 0.28 +1.9 1,179,922 + ycfZ b1121 0.28 +1.9 1,179,922 + ycfZ b1121 0.28 +1.9 1,179,922 + ycfZ b1121 0.28 +0.9 1,179,922 + ycfZ b1121 0.28 +0.3 1,179,943 + ycfZ b1121 0.31 +1.9 1,179,992 + ycfZ b1121 0.37 +1.9 1,180,234 - ycfZ b1121 0.67 +1.9 1,180,389 - ycfZ b1121 0.87 +1.9 1,180,389 - ycfZ b1121 0.87 +0.3 1,180,438 + +1.9 1,180,457 + +1.9 1,180,739 - ymfA b1122 0.55 +1.4 1,180,755 - ymfA b1122 0.58 -0.5 1,180,755 - ymfA b1122 0.58 +0.6 1,180,763 - ymfA b1122 0.60 -0.9 1,180,777 - ymfA b1122 0.63 -0.5 1,180,777 - ymfA b1122 0.63 +1.0 1,180,777 - ymfA b1122 0.63 -0.5 1,180,777 - ymfA b1122 0.63 +0.8 1,180,777 - ymfA b1122 0.63 -0.2 1,180,777 - ymfA b1122 0.63 +0.6 1,180,778 - ymfA b1122 0.63 +1.3 1,180,796 + ymfA b1122 0.67 -0.9 1,180,796 + ymfA b1122 0.67 -1.6 1,180,799 - ymfA b1122 0.68 -0.7 1,180,800 - ymfA b1122 0.68 +1.9 1,180,803 - ymfA b1122 0.68 -0.1 1,180,809 + ymfA b1122 0.70 +0.3 1,180,813 + ymfA b1122 0.71 +1.4 1,180,817 - ymfA b1122 0.71 +0.2 1,180,834 - ymfA b1122 0.75 +0.6 1,180,838 - ymfA b1122 0.76 +0.3 1,180,854 - ymfA b1122 0.79 +0.3 1,180,869 - ymfA b1122 0.83 -0.2 1,180,881 + ymfA b1122 0.85 +0.5 1,180,881 + ymfA b1122 0.85 -0.2 1,180,890 + ymfA b1122 0.87 +0.2 1,180,894 + ymfA b1122 0.88 -0.5 1,180,894 + ymfA b1122 0.88 +0.4 1,180,894 + ymfA b1122 0.88 +0.3 1,180,900 + ymfA b1122 0.89 +0.2 1,180,900 + ymfA b1122 0.89 +0.4 1,180,902 - -1.1 1,180,908 - +1.3 1,180,937 + +0.1 1,180,945 - +0.3 1,180,945 - -0.1 1,180,953 - -1.1 1,180,959 - +0.0 1,180,988 - -1.5 1,180,988 - -0.0 1,180,989 - -0.4 1,180,989 - -0.7 1,181,036 - +1.2 1,181,046 - +0.3 1,181,101 + -0.7 1,181,101 + -1.5 1,181,170 - potD b1123 0.16 -0.1 1,181,280 - potD b1123 0.26 -0.7 1,181,280 - potD b1123 0.26 -1.2 1,181,421 + potD b1123 0.40 -0.5 1,181,480 - potD b1123 0.45 -0.8
Or see this region's nucleotide sequence