Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrC and yceB are separated by 105 nucleotides yceB and grxB are separated by 133 nucleotides grxB and yceL are separated by 63 nucleotides
b1062: pyrC - dihydroorotase (NCBI), at 1,120,784 to 1,121,830
pyrC
b1063: yceB - predicted lipoprotein (NCBI), at 1,121,936 to 1,122,496
yceB
b1064: grxB - glutaredoxin 2 (Grx2) (NCBI), at 1,122,630 to 1,123,277
grxB
b1065: yceL - orf, hypothetical protein (VIMSS), at 1,123,341 to 1,124,549
yceL
Position (kb)
1121
1122
1123 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1120.985 kb on - strand, within pyrC at 1120.985 kb on - strand, within pyrC at 1121.019 kb on + strand, within pyrC at 1121.059 kb on + strand, within pyrC at 1121.073 kb on - strand, within pyrC at 1121.073 kb on - strand, within pyrC at 1121.123 kb on + strand, within pyrC at 1121.123 kb on + strand, within pyrC at 1121.174 kb on - strand, within pyrC at 1121.174 kb on - strand, within pyrC at 1121.195 kb on - strand, within pyrC at 1121.205 kb on - strand, within pyrC at 1121.205 kb on - strand, within pyrC at 1121.210 kb on + strand, within pyrC at 1121.427 kb on - strand, within pyrC at 1121.456 kb on + strand, within pyrC at 1121.495 kb on + strand, within pyrC at 1121.574 kb on - strand, within pyrC at 1121.574 kb on - strand, within pyrC at 1121.636 kb on + strand, within pyrC at 1121.641 kb on + strand, within pyrC at 1121.668 kb on + strand, within pyrC at 1121.668 kb on + strand, within pyrC at 1121.689 kb on + strand, within pyrC at 1121.689 kb on + strand, within pyrC at 1121.694 kb on + strand, within pyrC at 1121.793 kb on + strand at 1121.793 kb on + strand at 1122.106 kb on - strand, within yceB at 1122.106 kb on - strand, within yceB at 1122.256 kb on - strand, within yceB at 1122.315 kb on + strand, within yceB at 1122.325 kb on + strand, within yceB at 1122.356 kb on + strand, within yceB at 1122.356 kb on + strand, within yceB at 1122.501 kb on + strand at 1122.533 kb on + strand at 1122.546 kb on - strand at 1122.549 kb on - strand at 1122.556 kb on + strand at 1122.689 kb on + strand at 1122.829 kb on - strand, within grxB at 1122.942 kb on - strand, within grxB at 1123.018 kb on - strand, within grxB at 1123.099 kb on + strand, within grxB at 1123.099 kb on + strand, within grxB at 1123.099 kb on + strand, within grxB at 1123.195 kb on - strand, within grxB at 1123.274 kb on + strand at 1123.322 kb on + strand at 1123.322 kb on + strand at 1123.329 kb on - strand at 1123.443 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,120,985 - pyrC b1062 0.19 +0.3 1,120,985 - pyrC b1062 0.19 -0.6 1,121,019 + pyrC b1062 0.22 -1.0 1,121,059 + pyrC b1062 0.26 +1.4 1,121,073 - pyrC b1062 0.28 -0.4 1,121,073 - pyrC b1062 0.28 -0.9 1,121,123 + pyrC b1062 0.32 -2.0 1,121,123 + pyrC b1062 0.32 -0.0 1,121,174 - pyrC b1062 0.37 -0.2 1,121,174 - pyrC b1062 0.37 +0.3 1,121,195 - pyrC b1062 0.39 -0.5 1,121,205 - pyrC b1062 0.40 -2.5 1,121,205 - pyrC b1062 0.40 -0.0 1,121,210 + pyrC b1062 0.41 -0.0 1,121,427 - pyrC b1062 0.61 +0.4 1,121,456 + pyrC b1062 0.64 -0.6 1,121,495 + pyrC b1062 0.68 +0.1 1,121,574 - pyrC b1062 0.75 -2.1 1,121,574 - pyrC b1062 0.75 -1.2 1,121,636 + pyrC b1062 0.81 -0.7 1,121,641 + pyrC b1062 0.82 +0.0 1,121,668 + pyrC b1062 0.84 +1.4 1,121,668 + pyrC b1062 0.84 -1.0 1,121,689 + pyrC b1062 0.86 -1.0 1,121,689 + pyrC b1062 0.86 -0.2 1,121,694 + pyrC b1062 0.87 -0.5 1,121,793 + -0.2 1,121,793 + -0.3 1,122,106 - yceB b1063 0.30 +2.0 1,122,106 - yceB b1063 0.30 -0.6 1,122,256 - yceB b1063 0.57 +0.5 1,122,315 + yceB b1063 0.68 -0.7 1,122,325 + yceB b1063 0.69 +0.3 1,122,356 + yceB b1063 0.75 +0.8 1,122,356 + yceB b1063 0.75 -0.7 1,122,501 + -0.7 1,122,533 + -0.7 1,122,546 - +0.3 1,122,549 - -0.0 1,122,556 + -0.2 1,122,689 + -1.1 1,122,829 - grxB b1064 0.31 +0.4 1,122,942 - grxB b1064 0.48 -0.3 1,123,018 - grxB b1064 0.60 +0.3 1,123,099 + grxB b1064 0.72 -0.1 1,123,099 + grxB b1064 0.72 +1.1 1,123,099 + grxB b1064 0.72 -0.6 1,123,195 - grxB b1064 0.87 +0.5 1,123,274 + -0.9 1,123,322 + -0.2 1,123,322 + +0.6 1,123,329 - +1.7 1,123,443 + +0.3
Or see this region's nucleotide sequence