Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdS and ycdT are separated by 586 nucleotides ycdT and insF-4 are separated by 40 nucleotides insF-4 and insE overlap by 4 nucleotides
b1024: ycdS - predicted outer membrane protein (NCBI), at 1,089,089 to 1,091,512
ycdS
b1025: ycdT - predicted diguanylate cyclase (NCBI), at 1,092,099 to 1,093,457
ycdT
b1026: insF-4 - IS3 element protein InsF (NCBI), at 1,093,498 to 1,094,364
insF-4
b1027: insE - IS3 element protein InsE (RefSeq), at 1,094,361 to 1,094,660
insE
Position (kb)
1092
1093
1094 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1091.116 kb on - strand, within ycdS at 1091.116 kb on - strand, within ycdS at 1091.128 kb on + strand, within ycdS at 1091.136 kb on - strand, within ycdS at 1091.136 kb on - strand, within ycdS at 1091.138 kb on - strand, within ycdS at 1091.138 kb on - strand, within ycdS at 1091.138 kb on - strand, within ycdS at 1091.138 kb on - strand, within ycdS at 1091.138 kb on - strand, within ycdS at 1091.153 kb on - strand, within ycdS at 1091.155 kb on + strand, within ycdS at 1091.155 kb on + strand, within ycdS at 1091.155 kb on + strand, within ycdS at 1091.155 kb on - strand, within ycdS at 1091.155 kb on - strand, within ycdS at 1091.161 kb on - strand, within ycdS at 1091.163 kb on - strand, within ycdS at 1091.163 kb on - strand, within ycdS at 1091.163 kb on - strand, within ycdS at 1091.163 kb on - strand, within ycdS at 1091.163 kb on - strand, within ycdS at 1091.163 kb on - strand, within ycdS at 1091.176 kb on + strand, within ycdS at 1091.176 kb on + strand, within ycdS at 1091.176 kb on + strand, within ycdS at 1091.177 kb on - strand, within ycdS at 1091.182 kb on + strand, within ycdS at 1091.182 kb on + strand, within ycdS at 1091.182 kb on + strand, within ycdS at 1091.182 kb on - strand, within ycdS at 1091.191 kb on - strand, within ycdS at 1091.195 kb on - strand, within ycdS at 1091.206 kb on + strand, within ycdS at 1091.206 kb on + strand, within ycdS at 1091.213 kb on + strand, within ycdS at 1091.213 kb on + strand, within ycdS at 1091.214 kb on - strand, within ycdS at 1091.223 kb on + strand, within ycdS at 1091.223 kb on + strand, within ycdS at 1091.233 kb on + strand, within ycdS at 1091.238 kb on + strand, within ycdS at 1091.238 kb on + strand, within ycdS at 1091.256 kb on + strand, within ycdS at 1091.263 kb on + strand, within ycdS at 1091.264 kb on - strand, within ycdS at 1091.283 kb on - strand at 1091.287 kb on + strand at 1091.290 kb on - strand at 1091.293 kb on + strand at 1091.301 kb on - strand at 1091.301 kb on - strand at 1091.301 kb on - strand at 1091.343 kb on + strand at 1091.353 kb on - strand at 1091.364 kb on + strand at 1091.364 kb on + strand at 1091.372 kb on - strand at 1091.405 kb on - strand at 1091.410 kb on + strand at 1091.413 kb on - strand at 1091.423 kb on - strand at 1091.425 kb on + strand at 1091.428 kb on - strand at 1091.432 kb on + strand at 1091.447 kb on - strand at 1091.511 kb on + strand at 1091.516 kb on + strand at 1091.519 kb on - strand at 1091.524 kb on - strand at 1091.528 kb on - strand at 1091.529 kb on - strand at 1091.529 kb on - strand at 1091.661 kb on + strand at 1091.661 kb on + strand at 1091.715 kb on - strand at 1091.716 kb on + strand at 1091.824 kb on + strand at 1091.832 kb on - strand at 1091.961 kb on - strand at 1091.998 kb on + strand at 1092.001 kb on + strand at 1092.004 kb on + strand at 1092.033 kb on + strand at 1092.071 kb on + strand at 1092.071 kb on + strand at 1092.074 kb on + strand at 1092.079 kb on + strand at 1092.079 kb on + strand at 1092.084 kb on + strand at 1092.086 kb on + strand at 1092.088 kb on + strand at 1092.089 kb on + strand at 1092.089 kb on + strand at 1092.089 kb on + strand at 1092.089 kb on + strand at 1092.089 kb on + strand at 1092.089 kb on + strand at 1092.097 kb on - strand at 1092.132 kb on + strand at 1092.141 kb on + strand at 1092.159 kb on + strand at 1092.222 kb on + strand at 1092.226 kb on + strand at 1093.014 kb on - strand, within ycdT at 1093.052 kb on - strand, within ycdT at 1093.087 kb on - strand, within ycdT at 1093.168 kb on - strand, within ycdT at 1093.210 kb on + strand, within ycdT at 1093.210 kb on + strand, within ycdT at 1093.232 kb on + strand, within ycdT at 1093.312 kb on + strand, within ycdT at 1093.323 kb on + strand at 1093.323 kb on + strand at 1093.323 kb on + strand at 1093.331 kb on - strand at 1093.375 kb on + strand at 1093.375 kb on + strand at 1093.379 kb on - strand at 1093.416 kb on - strand at 1093.417 kb on - strand at 1093.417 kb on - strand at 1093.419 kb on + strand at 1093.419 kb on + strand at 1093.422 kb on + strand at 1093.426 kb on - strand at 1093.427 kb on - strand at 1093.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,091,116 - ycdS b1024 0.84 +1.0 1,091,116 - ycdS b1024 0.84 -0.7 1,091,128 + ycdS b1024 0.84 -0.0 1,091,136 - ycdS b1024 0.84 +0.5 1,091,136 - ycdS b1024 0.84 -0.1 1,091,138 - ycdS b1024 0.85 -0.9 1,091,138 - ycdS b1024 0.85 -0.2 1,091,138 - ycdS b1024 0.85 +0.4 1,091,138 - ycdS b1024 0.85 +0.8 1,091,138 - ycdS b1024 0.85 -1.9 1,091,153 - ycdS b1024 0.85 +0.2 1,091,155 + ycdS b1024 0.85 -0.3 1,091,155 + ycdS b1024 0.85 +0.4 1,091,155 + ycdS b1024 0.85 -0.0 1,091,155 - ycdS b1024 0.85 -1.5 1,091,155 - ycdS b1024 0.85 -1.6 1,091,161 - ycdS b1024 0.85 +0.6 1,091,163 - ycdS b1024 0.86 +0.3 1,091,163 - ycdS b1024 0.86 +0.9 1,091,163 - ycdS b1024 0.86 +0.3 1,091,163 - ycdS b1024 0.86 +1.0 1,091,163 - ycdS b1024 0.86 -0.5 1,091,163 - ycdS b1024 0.86 +1.0 1,091,176 + ycdS b1024 0.86 -1.0 1,091,176 + ycdS b1024 0.86 +1.2 1,091,176 + ycdS b1024 0.86 +0.6 1,091,177 - ycdS b1024 0.86 +2.0 1,091,182 + ycdS b1024 0.86 -1.6 1,091,182 + ycdS b1024 0.86 +0.7 1,091,182 + ycdS b1024 0.86 -2.7 1,091,182 - ycdS b1024 0.86 +1.6 1,091,191 - ycdS b1024 0.87 -0.0 1,091,195 - ycdS b1024 0.87 +0.8 1,091,206 + ycdS b1024 0.87 +0.7 1,091,206 + ycdS b1024 0.87 -0.3 1,091,213 + ycdS b1024 0.88 -0.3 1,091,213 + ycdS b1024 0.88 -0.5 1,091,214 - ycdS b1024 0.88 -0.8 1,091,223 + ycdS b1024 0.88 +0.2 1,091,223 + ycdS b1024 0.88 +0.5 1,091,233 + ycdS b1024 0.88 +1.0 1,091,238 + ycdS b1024 0.89 -0.0 1,091,238 + ycdS b1024 0.89 -1.3 1,091,256 + ycdS b1024 0.89 +1.6 1,091,263 + ycdS b1024 0.90 -0.6 1,091,264 - ycdS b1024 0.90 -0.6 1,091,283 - -0.1 1,091,287 + +0.8 1,091,290 - -1.2 1,091,293 + +1.1 1,091,301 - +0.5 1,091,301 - +1.3 1,091,301 - +1.2 1,091,343 + +0.6 1,091,353 - +1.6 1,091,364 + -0.3 1,091,364 + +0.1 1,091,372 - +2.0 1,091,405 - +1.4 1,091,410 + +2.0 1,091,413 - +1.0 1,091,423 - +0.2 1,091,425 + +1.9 1,091,428 - +3.0 1,091,432 + -0.4 1,091,447 - +0.6 1,091,511 + +2.0 1,091,516 + +1.0 1,091,519 - -0.7 1,091,524 - +2.8 1,091,528 - -0.4 1,091,529 - +1.4 1,091,529 - -1.6 1,091,661 + -0.5 1,091,661 + +2.2 1,091,715 - +1.0 1,091,716 + +2.4 1,091,824 + +2.0 1,091,832 - +3.0 1,091,961 - +0.4 1,091,998 + +2.0 1,092,001 + -0.0 1,092,004 + +0.4 1,092,033 + -0.3 1,092,071 + +0.9 1,092,071 + -0.3 1,092,074 + -0.0 1,092,079 + -0.2 1,092,079 + +0.8 1,092,084 + -0.9 1,092,086 + -0.1 1,092,088 + -0.5 1,092,089 + +1.2 1,092,089 + -1.7 1,092,089 + +0.4 1,092,089 + +0.4 1,092,089 + -1.0 1,092,089 + -0.8 1,092,097 - -0.0 1,092,132 + +2.0 1,092,141 + +1.0 1,092,159 + +2.0 1,092,222 + +2.0 1,092,226 + +2.0 1,093,014 - ycdT b1025 0.67 +0.4 1,093,052 - ycdT b1025 0.70 -0.0 1,093,087 - ycdT b1025 0.73 -0.0 1,093,168 - ycdT b1025 0.79 -0.0 1,093,210 + ycdT b1025 0.82 -0.8 1,093,210 + ycdT b1025 0.82 -0.0 1,093,232 + ycdT b1025 0.83 -0.8 1,093,312 + ycdT b1025 0.89 +1.4 1,093,323 + -0.5 1,093,323 + -0.7 1,093,323 + -0.3 1,093,331 - -0.0 1,093,375 + +0.2 1,093,375 + -0.3 1,093,379 - +2.0 1,093,416 - +2.0 1,093,417 - +1.0 1,093,417 - +2.0 1,093,419 + -0.3 1,093,419 + -1.2 1,093,422 + +1.6 1,093,426 - +0.8 1,093,427 - +0.4 1,093,493 - +0.2
Or see this region's nucleotide sequence