Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pqiB and ymbA overlap by 4 nucleotides ymbA and rmf are separated by 255 nucleotides rmf and fabA are separated by 69 nucleotides fabA and ycbZ are separated by 68 nucleotides
b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122
pqiB
b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682
ymbA
b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105
rmf
b0954: fabA - 3-hydroxydecanoyl-ACP dehydratase (NCBI), at 1,015,175 to 1,015,693
fabA
b0955: ycbZ - predicted peptidase (NCBI), at 1,015,762 to 1,017,522
ycbZ
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-2
-1
0
1 at 1013.949 kb on - strand, within pqiB at 1013.955 kb on + strand, within pqiB at 1013.955 kb on + strand, within pqiB at 1014.064 kb on - strand at 1014.064 kb on - strand at 1014.159 kb on - strand at 1014.206 kb on + strand, within ymbA at 1014.216 kb on + strand, within ymbA at 1014.216 kb on + strand, within ymbA at 1014.263 kb on + strand, within ymbA at 1014.268 kb on + strand, within ymbA at 1014.303 kb on - strand, within ymbA at 1014.428 kb on - strand, within ymbA at 1014.428 kb on - strand, within ymbA at 1014.587 kb on + strand, within ymbA at 1014.588 kb on + strand, within ymbA at 1014.588 kb on + strand, within ymbA at 1014.619 kb on - strand, within ymbA at 1014.959 kb on + strand, within rmf at 1014.974 kb on + strand, within rmf at 1014.974 kb on + strand, within rmf at 1015.089 kb on + strand at 1015.097 kb on - strand at 1015.736 kb on - strand at 1015.736 kb on - strand at 1015.736 kb on - strand at 1015.740 kb on - strand at 1015.740 kb on - strand at 1015.876 kb on + strand at 1015.878 kb on - strand at 1015.894 kb on - strand at 1015.922 kb on - strand at 1015.935 kb on + strand at 1015.967 kb on - strand, within ycbZ at 1015.967 kb on - strand, within ycbZ at 1015.989 kb on + strand, within ycbZ at 1016.003 kb on + strand, within ycbZ at 1016.003 kb on + strand, within ycbZ at 1016.012 kb on + strand, within ycbZ
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 1,013,949 - pqiB b0951 0.89 -0.0 1,013,955 + pqiB b0951 0.90 -1.1 1,013,955 + pqiB b0951 0.90 -0.3 1,014,064 - -0.4 1,014,064 - +0.2 1,014,159 - +0.2 1,014,206 + ymbA b0952 0.15 +0.2 1,014,216 + ymbA b0952 0.17 +0.3 1,014,216 + ymbA b0952 0.17 +0.5 1,014,263 + ymbA b0952 0.26 -0.0 1,014,268 + ymbA b0952 0.26 -1.2 1,014,303 - ymbA b0952 0.33 -0.1 1,014,428 - ymbA b0952 0.55 +0.0 1,014,428 - ymbA b0952 0.55 -0.4 1,014,587 + ymbA b0952 0.83 -0.1 1,014,588 + ymbA b0952 0.83 +0.5 1,014,588 + ymbA b0952 0.83 -0.2 1,014,619 - ymbA b0952 0.89 +0.3 1,014,959 + rmf b0953 0.12 +0.4 1,014,974 + rmf b0953 0.21 -1.0 1,014,974 + rmf b0953 0.21 +0.7 1,015,089 + +0.0 1,015,097 - +0.0 1,015,736 - -0.1 1,015,736 - +0.4 1,015,736 - +1.0 1,015,740 - +0.1 1,015,740 - +0.2 1,015,876 + -0.1 1,015,878 - -0.9 1,015,894 - -0.8 1,015,922 - -2.3 1,015,935 + -0.6 1,015,967 - ycbZ b0955 0.12 -0.0 1,015,967 - ycbZ b0955 0.12 -0.2 1,015,989 + ycbZ b0955 0.13 -0.3 1,016,003 + ycbZ b0955 0.14 -0.1 1,016,003 + ycbZ b0955 0.14 -0.3 1,016,012 + ycbZ b0955 0.14 -0.2
Or see this region's nucleotide sequence