Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoE and cyoD are separated by 11 nucleotides cyoD and cyoC overlap by 1 nucleotides cyoC and cyoB overlap by 11 nucleotides
b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929
cyoE
b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270
cyoD
b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884
cyoC
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
Position (kb) 446
447
448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 445.978 kb on + strand at 446.028 kb on - strand at 446.028 kb on - strand at 446.054 kb on + strand at 446.069 kb on - strand at 446.069 kb on - strand at 446.096 kb on + strand at 446.101 kb on - strand at 446.119 kb on + strand at 446.140 kb on - strand, within cyoE at 446.161 kb on - strand, within cyoE at 446.242 kb on - strand, within cyoE at 446.297 kb on - strand, within cyoE at 446.394 kb on + strand, within cyoE at 446.394 kb on + strand, within cyoE at 446.394 kb on + strand, within cyoE at 446.407 kb on - strand, within cyoE at 446.525 kb on + strand, within cyoE at 446.525 kb on + strand, within cyoE at 446.533 kb on - strand, within cyoE at 446.562 kb on - strand, within cyoE at 446.562 kb on - strand, within cyoE at 446.657 kb on + strand, within cyoE at 446.657 kb on + strand, within cyoE at 446.673 kb on - strand, within cyoE at 446.677 kb on + strand, within cyoE at 446.677 kb on + strand, within cyoE at 446.796 kb on - strand, within cyoE at 446.824 kb on + strand, within cyoE at 446.832 kb on - strand, within cyoE at 446.880 kb on + strand at 446.880 kb on + strand at 446.932 kb on + strand at 446.944 kb on + strand at 446.981 kb on - strand, within cyoD at 447.018 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.105 kb on - strand, within cyoD at 447.161 kb on - strand, within cyoD at 447.167 kb on + strand, within cyoD at 447.175 kb on - strand, within cyoD at 447.201 kb on + strand, within cyoD at 447.223 kb on - strand, within cyoD at 447.285 kb on - strand at 447.303 kb on - strand at 447.347 kb on + strand, within cyoC at 447.351 kb on + strand, within cyoC at 447.373 kb on - strand, within cyoC at 447.407 kb on + strand, within cyoC at 447.418 kb on - strand, within cyoC at 447.445 kb on - strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.521 kb on + strand, within cyoC at 447.570 kb on + strand, within cyoC at 447.580 kb on + strand, within cyoC at 447.679 kb on + strand, within cyoC at 447.956 kb on + strand at 447.964 kb on - strand at 447.964 kb on - strand at 447.989 kb on + strand at 448.002 kb on - strand at 448.005 kb on + strand at 448.092 kb on - strand, within cyoB at 448.092 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.238 kb on - strand, within cyoB
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 445,978 + -0.5 446,028 - -0.5 446,028 - -1.4 446,054 + +0.6 446,069 - -0.2 446,069 - +0.9 446,096 + +0.7 446,101 - -1.0 446,119 + -0.2 446,140 - cyoE b0428 0.11 -0.8 446,161 - cyoE b0428 0.14 -0.7 446,242 - cyoE b0428 0.23 -0.6 446,297 - cyoE b0428 0.29 -0.1 446,394 + cyoE b0428 0.40 -1.1 446,394 + cyoE b0428 0.40 -1.5 446,394 + cyoE b0428 0.40 -0.7 446,407 - cyoE b0428 0.41 -1.4 446,525 + cyoE b0428 0.55 +0.1 446,525 + cyoE b0428 0.55 -0.1 446,533 - cyoE b0428 0.55 -0.1 446,562 - cyoE b0428 0.59 +0.6 446,562 - cyoE b0428 0.59 +0.1 446,657 + cyoE b0428 0.69 -0.8 446,657 + cyoE b0428 0.69 -0.4 446,673 - cyoE b0428 0.71 +0.2 446,677 + cyoE b0428 0.72 -0.5 446,677 + cyoE b0428 0.72 +3.3 446,796 - cyoE b0428 0.85 -0.8 446,824 + cyoE b0428 0.88 -0.8 446,832 - cyoE b0428 0.89 +0.8 446,880 + +0.3 446,880 + -0.8 446,932 + +0.0 446,944 + -1.1 446,981 - cyoD b0429 0.12 -0.4 447,018 - cyoD b0429 0.23 -0.5 447,093 - cyoD b0429 0.46 +0.2 447,093 - cyoD b0429 0.46 -2.6 447,105 - cyoD b0429 0.50 -1.2 447,161 - cyoD b0429 0.67 -0.7 447,167 + cyoD b0429 0.68 -0.2 447,175 - cyoD b0429 0.71 -0.1 447,201 + cyoD b0429 0.79 -0.5 447,223 - cyoD b0429 0.85 -1.3 447,285 - -0.7 447,303 - -1.1 447,347 + cyoC b0430 0.13 -0.7 447,351 + cyoC b0430 0.13 -0.5 447,373 - cyoC b0430 0.17 -0.6 447,407 + cyoC b0430 0.22 +0.2 447,418 - cyoC b0430 0.24 -2.2 447,445 - cyoC b0430 0.28 +0.0 447,492 + cyoC b0430 0.36 -0.1 447,492 + cyoC b0430 0.36 -0.6 447,521 + cyoC b0430 0.41 -0.6 447,570 + cyoC b0430 0.49 -1.5 447,580 + cyoC b0430 0.50 -0.6 447,679 + cyoC b0430 0.67 -0.3 447,956 + -1.1 447,964 - +0.7 447,964 - +0.0 447,989 + +0.2 448,002 - +0.3 448,005 + -0.5 448,092 - cyoB b0431 0.11 -0.9 448,092 - cyoB b0431 0.11 +1.6 448,134 - cyoB b0431 0.13 -0.8 448,134 - cyoB b0431 0.13 -2.8 448,238 - cyoB b0431 0.18 -0.7
Or see this region's nucleotide sequence