Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaiW and yaiY are separated by 58 nucleotides yaiY and b0380 are separated by 259 nucleotides b0380 and ddlA are separated by 23 nucleotides
b0378: yaiW - predicted DNA-binding transcriptional regulator (NCBI), at 397,096 to 398,190
yaiW
b0379: yaiY - predicted inner membrane protein (NCBI), at 398,249 to 398,557
yaiY
b0380: b0380 - orf, hypothetical protein (VIMSS), at 398,817 to 399,029
b0380
b0381: ddlA - D-alanylalanine synthetase (NCBI), at 399,053 to 400,147
ddlA
Position (kb) 398
399
400 Strain fitness (log2 ratio) -1
0
1
2 at 397.818 kb on + strand, within yaiW at 397.847 kb on + strand, within yaiW at 397.847 kb on + strand, within yaiW at 397.891 kb on + strand, within yaiW at 397.987 kb on + strand, within yaiW at 398.006 kb on + strand, within yaiW at 398.025 kb on + strand, within yaiW at 398.025 kb on + strand, within yaiW at 398.318 kb on - strand, within yaiY at 398.351 kb on + strand, within yaiY at 398.386 kb on - strand, within yaiY at 398.386 kb on - strand, within yaiY at 398.392 kb on - strand, within yaiY at 398.392 kb on - strand, within yaiY at 398.593 kb on - strand at 398.608 kb on + strand at 398.613 kb on - strand at 398.633 kb on - strand at 398.755 kb on + strand at 398.755 kb on + strand at 398.809 kb on + strand at 398.852 kb on + strand, within b0380 at 398.860 kb on - strand, within b0380 at 398.860 kb on - strand, within b0380 at 398.883 kb on + strand, within b0380 at 398.933 kb on + strand, within b0380 at 398.933 kb on + strand, within b0380 at 398.933 kb on + strand, within b0380 at 398.959 kb on - strand, within b0380 at 398.959 kb on - strand, within b0380 at 399.034 kb on + strand at 399.034 kb on + strand at 399.079 kb on + strand at 399.092 kb on - strand at 399.092 kb on - strand at 399.129 kb on + strand at 399.129 kb on + strand at 399.129 kb on + strand at 399.137 kb on - strand at 399.137 kb on - strand at 399.217 kb on + strand, within ddlA at 399.376 kb on - strand, within ddlA at 399.376 kb on - strand, within ddlA at 399.531 kb on + strand, within ddlA at 399.549 kb on - strand, within ddlA at 399.549 kb on - strand, within ddlA at 399.659 kb on - strand, within ddlA at 399.707 kb on - strand, within ddlA at 399.727 kb on + strand, within ddlA at 399.727 kb on + strand, within ddlA at 399.787 kb on + strand, within ddlA at 399.824 kb on + strand, within ddlA at 399.916 kb on + strand, within ddlA at 399.933 kb on + strand, within ddlA at 399.948 kb on + strand, within ddlA at 399.948 kb on + strand, within ddlA
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 397,818 + yaiW b0378 0.66 +0.2 397,847 + yaiW b0378 0.69 +0.1 397,847 + yaiW b0378 0.69 -0.5 397,891 + yaiW b0378 0.73 -0.1 397,987 + yaiW b0378 0.81 -0.5 398,006 + yaiW b0378 0.83 -0.1 398,025 + yaiW b0378 0.85 -0.2 398,025 + yaiW b0378 0.85 -0.3 398,318 - yaiY b0379 0.22 +0.1 398,351 + yaiY b0379 0.33 -0.2 398,386 - yaiY b0379 0.44 -1.0 398,386 - yaiY b0379 0.44 -0.2 398,392 - yaiY b0379 0.46 +0.6 398,392 - yaiY b0379 0.46 -0.3 398,593 - -0.2 398,608 + +0.2 398,613 - +0.6 398,633 - +0.2 398,755 + -0.4 398,755 + -0.4 398,809 + +0.4 398,852 + b0380 b0380 0.16 +0.1 398,860 - b0380 b0380 0.20 -0.3 398,860 - b0380 b0380 0.20 -1.2 398,883 + b0380 b0380 0.31 +0.4 398,933 + b0380 b0380 0.54 +0.8 398,933 + b0380 b0380 0.54 +0.9 398,933 + b0380 b0380 0.54 +1.1 398,959 - b0380 b0380 0.67 +2.5 398,959 - b0380 b0380 0.67 -1.7 399,034 + -0.4 399,034 + -0.3 399,079 + +0.1 399,092 - +0.3 399,092 - +0.7 399,129 + +0.4 399,129 + +0.9 399,129 + -0.3 399,137 - +0.6 399,137 - +1.2 399,217 + ddlA b0381 0.15 -0.8 399,376 - ddlA b0381 0.29 +0.8 399,376 - ddlA b0381 0.29 -1.7 399,531 + ddlA b0381 0.44 +0.3 399,549 - ddlA b0381 0.45 -0.2 399,549 - ddlA b0381 0.45 +0.0 399,659 - ddlA b0381 0.55 -0.3 399,707 - ddlA b0381 0.60 -0.6 399,727 + ddlA b0381 0.62 -0.3 399,727 + ddlA b0381 0.62 +0.8 399,787 + ddlA b0381 0.67 +0.8 399,824 + ddlA b0381 0.70 +0.3 399,916 + ddlA b0381 0.79 +0.0 399,933 + ddlA b0381 0.80 +0.2 399,948 + ddlA b0381 0.82 -1.0 399,948 + ddlA b0381 0.82 +0.7
Or see this region's nucleotide sequence