Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykfD and mmuM overlap by 14 nucleotides mmuM and afuC are separated by 108 nucleotides
b0260: ykfD - putative amino acid/amine transport protein (VIMSS), at 274,549 to 275,952
ykfD
b0261: mmuM - homocysteine methyltransferase (NCBI), at 275,939 to 276,871
mmuM
b0262: afuC - putative ATP-binding component of a transport system (VIMSS), at 276,980 to 278,026
afuC
Position (kb) 275
276
277 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.950 kb on - strand, within ykfD at 274.950 kb on - strand, within ykfD at 274.955 kb on - strand, within ykfD at 275.190 kb on - strand, within ykfD at 275.516 kb on - strand, within ykfD at 275.585 kb on - strand, within ykfD at 275.627 kb on - strand, within ykfD at 275.883 kb on + strand at 275.883 kb on + strand at 275.976 kb on - strand at 275.986 kb on - strand at 275.988 kb on - strand at 275.988 kb on - strand at 276.000 kb on - strand at 276.059 kb on + strand, within mmuM at 276.073 kb on - strand, within mmuM at 276.073 kb on - strand, within mmuM at 276.333 kb on + strand, within mmuM at 276.346 kb on - strand, within mmuM at 276.346 kb on - strand, within mmuM at 276.358 kb on + strand, within mmuM at 276.415 kb on + strand, within mmuM at 276.580 kb on - strand, within mmuM at 276.621 kb on + strand, within mmuM at 276.621 kb on + strand, within mmuM at 276.669 kb on + strand, within mmuM at 276.677 kb on - strand, within mmuM at 276.682 kb on + strand, within mmuM at 276.685 kb on + strand, within mmuM at 276.882 kb on + strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.890 kb on - strand at 276.931 kb on + strand at 277.032 kb on - strand at 277.206 kb on - strand, within afuC at 277.208 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.296 kb on - strand, within afuC at 277.311 kb on - strand, within afuC at 277.311 kb on - strand, within afuC at 277.410 kb on + strand, within afuC at 277.410 kb on + strand, within afuC at 277.427 kb on + strand, within afuC at 277.572 kb on - strand, within afuC at 277.619 kb on - strand, within afuC at 277.619 kb on - strand, within afuC at 277.682 kb on + strand, within afuC at 277.785 kb on - strand, within afuC at 277.787 kb on + strand, within afuC at 277.801 kb on + strand, within afuC at 277.804 kb on - strand, within afuC at 277.804 kb on - strand, within afuC at 277.809 kb on - strand, within afuC at 277.809 kb on - strand, within afuC
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 274,950 - ykfD b0260 0.29 +1.9 274,950 - ykfD b0260 0.29 -1.1 274,955 - ykfD b0260 0.29 +0.2 275,190 - ykfD b0260 0.46 -0.2 275,516 - ykfD b0260 0.69 -0.4 275,585 - ykfD b0260 0.74 +0.2 275,627 - ykfD b0260 0.77 -0.6 275,883 + -0.7 275,883 + +0.5 275,976 - +0.0 275,986 - +0.4 275,988 - -0.0 275,988 - +0.1 276,000 - +0.3 276,059 + mmuM b0261 0.13 +0.4 276,073 - mmuM b0261 0.14 +0.1 276,073 - mmuM b0261 0.14 +0.6 276,333 + mmuM b0261 0.42 +0.9 276,346 - mmuM b0261 0.44 +0.2 276,346 - mmuM b0261 0.44 +0.6 276,358 + mmuM b0261 0.45 +0.4 276,415 + mmuM b0261 0.51 +1.0 276,580 - mmuM b0261 0.69 -0.9 276,621 + mmuM b0261 0.73 +0.1 276,621 + mmuM b0261 0.73 -0.1 276,669 + mmuM b0261 0.78 +0.8 276,677 - mmuM b0261 0.79 -0.2 276,682 + mmuM b0261 0.80 +0.2 276,685 + mmuM b0261 0.80 +0.3 276,882 + +0.2 276,890 - -0.2 276,890 - -1.0 276,890 - -2.8 276,890 - +0.2 276,931 + +0.1 277,032 - -0.6 277,206 - afuC b0262 0.22 -0.7 277,208 + afuC b0262 0.22 -0.2 277,225 + afuC b0262 0.23 -1.0 277,225 + afuC b0262 0.23 -0.1 277,225 + afuC b0262 0.23 -0.9 277,225 + afuC b0262 0.23 +0.4 277,225 + afuC b0262 0.23 -2.1 277,296 - afuC b0262 0.30 -0.3 277,311 - afuC b0262 0.32 -0.6 277,311 - afuC b0262 0.32 +0.1 277,410 + afuC b0262 0.41 -0.4 277,410 + afuC b0262 0.41 -0.0 277,427 + afuC b0262 0.43 -0.3 277,572 - afuC b0262 0.57 -0.3 277,619 - afuC b0262 0.61 -0.5 277,619 - afuC b0262 0.61 +1.6 277,682 + afuC b0262 0.67 +0.1 277,785 - afuC b0262 0.77 +0.4 277,787 + afuC b0262 0.77 +0.1 277,801 + afuC b0262 0.78 -1.1 277,804 - afuC b0262 0.79 +0.4 277,804 - afuC b0262 0.79 +0.8 277,809 - afuC b0262 0.79 -0.2 277,809 - afuC b0262 0.79 -0.3
Or see this region's nucleotide sequence