Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secA and mutT are separated by 59 nucleotides mutT and yacG are separated by 215 nucleotides yacG and yacF are separated by 9 nucleotides
b0098: secA - translocase (NCBI), at 108,279 to 110,984
secA
b0099: mutT - nucleoside triphosphate pyrophosphohydrolase, marked preference for dGTP (NCBI), at 111,044 to 111,433
mutT
b0101: yacG - zinc-binding protein (NCBI), at 111,649 to 111,846
yacG
b0102: yacF - hypothetical protein (NCBI), at 111,856 to 112,599
yacF
Position (kb) 111
112 Strain fitness (log2 ratio) -1
0
1 at 110.787 kb on + strand at 110.846 kb on + strand at 111.014 kb on + strand at 111.266 kb on + strand, within mutT at 111.276 kb on + strand, within mutT at 111.279 kb on + strand, within mutT at 111.328 kb on + strand, within mutT at 111.336 kb on - strand, within mutT at 111.426 kb on - strand at 111.426 kb on - strand at 111.504 kb on - strand at 111.504 kb on - strand at 111.616 kb on - strand at 111.792 kb on - strand, within yacG at 111.795 kb on + strand, within yacG at 111.795 kb on + strand, within yacG at 111.823 kb on + strand, within yacG at 111.828 kb on + strand at 111.828 kb on + strand at 111.836 kb on - strand at 111.836 kb on - strand at 111.886 kb on + strand at 111.995 kb on - strand, within yacF at 111.997 kb on + strand, within yacF at 112.005 kb on - strand at 112.005 kb on - strand, within yacF at 112.089 kb on - strand, within yacF at 112.089 kb on - strand, within yacF at 112.155 kb on + strand, within yacF at 112.188 kb on - strand, within yacF at 112.347 kb on + strand, within yacF at 112.347 kb on + strand, within yacF at 112.347 kb on + strand, within yacF at 112.366 kb on + strand, within yacF at 112.397 kb on - strand, within yacF
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 110,787 + -0.0 110,846 + +0.5 111,014 + +1.1 111,266 + mutT b0099 0.57 -0.5 111,276 + mutT b0099 0.59 -0.9 111,279 + mutT b0099 0.60 -0.4 111,328 + mutT b0099 0.73 -0.4 111,336 - mutT b0099 0.75 -1.0 111,426 - -1.1 111,426 - +0.4 111,504 - +0.0 111,504 - +1.1 111,616 - -0.7 111,792 - yacG b0101 0.72 -0.4 111,795 + yacG b0101 0.74 +0.4 111,795 + yacG b0101 0.74 -0.3 111,823 + yacG b0101 0.88 -0.2 111,828 + +0.9 111,828 + -0.6 111,836 - +0.2 111,836 - +0.8 111,886 + +0.6 111,995 - yacF b0102 0.19 +0.3 111,997 + yacF b0102 0.19 +0.2 112,005 - -0.0 112,005 - yacF b0102 0.20 +0.8 112,089 - yacF b0102 0.31 -0.7 112,089 - yacF b0102 0.31 -0.1 112,155 + yacF b0102 0.40 -0.3 112,188 - yacF b0102 0.45 -0.1 112,347 + yacF b0102 0.66 -0.2 112,347 + yacF b0102 0.66 +0.6 112,347 + yacF b0102 0.66 +0.5 112,366 + yacF b0102 0.69 -0.1 112,397 - yacF b0102 0.73 -0.4
Or see this region's nucleotide sequence