Experiment: T7_phage 0.0000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt caiT and fixA are separated by 471 nucleotides fixA and fixB are separated by 14 nucleotides
b0040: caiT - L-carnitine/gamma-butyrobetaine antiporter (NCBI), at 40,417 to 41,931
caiT
b0041: fixA - probable flavoprotein subunit, carnitine metabolism (VIMSS), at 42,403 to 43,173
fixA
b0042: fixB - predicted electron transfer flavoprotein, NAD/FAD-binding domain and ETFP adenine nucleotide-binding domain-like (NCBI), at 43,188 to 44,129
fixB
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 41.409 kb on + strand, within caiT at 41.411 kb on + strand, within caiT at 41.417 kb on - strand, within caiT at 41.417 kb on - strand, within caiT at 41.487 kb on - strand, within caiT at 41.574 kb on - strand, within caiT at 41.709 kb on - strand, within caiT at 41.709 kb on - strand, within caiT at 41.714 kb on + strand, within caiT at 41.716 kb on + strand, within caiT at 41.716 kb on + strand, within caiT at 41.723 kb on - strand, within caiT at 41.726 kb on + strand, within caiT at 41.726 kb on + strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.734 kb on - strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.753 kb on + strand, within caiT at 41.757 kb on - strand, within caiT at 41.757 kb on - strand, within caiT at 41.758 kb on + strand, within caiT at 41.759 kb on - strand, within caiT at 41.761 kb on + strand, within caiT at 41.761 kb on + strand, within caiT at 41.761 kb on - strand, within caiT at 41.761 kb on - strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.771 kb on + strand, within caiT at 41.774 kb on + strand, within caiT at 41.779 kb on + strand, within caiT at 41.783 kb on + strand at 41.786 kb on - strand at 41.791 kb on - strand at 41.791 kb on - strand at 41.794 kb on - strand at 41.795 kb on + strand at 41.797 kb on + strand at 41.803 kb on - strand at 41.830 kb on + strand at 41.830 kb on + strand at 41.830 kb on + strand at 41.838 kb on - strand at 41.838 kb on - strand at 41.844 kb on - strand at 41.846 kb on - strand at 41.849 kb on + strand at 41.850 kb on + strand at 41.938 kb on + strand at 42.028 kb on + strand at 42.028 kb on + strand at 42.028 kb on + strand at 42.040 kb on + strand at 42.041 kb on + strand at 42.041 kb on + strand at 42.041 kb on + strand at 42.041 kb on + strand at 42.043 kb on + strand at 42.048 kb on - strand at 42.049 kb on - strand at 42.059 kb on - strand at 42.070 kb on - strand at 42.070 kb on - strand at 42.070 kb on - strand at 42.070 kb on - strand at 42.097 kb on + strand at 42.098 kb on + strand at 42.098 kb on + strand at 42.430 kb on - strand at 42.442 kb on - strand at 42.446 kb on + strand at 42.447 kb on - strand at 42.452 kb on - strand at 42.452 kb on - strand at 42.456 kb on + strand at 42.464 kb on - strand at 42.464 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.466 kb on - strand at 42.470 kb on + strand at 42.478 kb on - strand at 42.478 kb on - strand at 42.478 kb on - strand at 42.485 kb on + strand, within fixA at 42.488 kb on - strand, within fixA at 42.491 kb on - strand, within fixA at 42.491 kb on - strand, within fixA at 42.534 kb on - strand, within fixA at 42.541 kb on + strand, within fixA at 42.550 kb on - strand, within fixA at 42.591 kb on - strand, within fixA at 42.601 kb on - strand, within fixA at 42.606 kb on - strand, within fixA at 42.682 kb on - strand, within fixA at 42.745 kb on + strand, within fixA at 42.873 kb on - strand, within fixA at 43.044 kb on + strand, within fixA at 43.058 kb on + strand, within fixA at 43.087 kb on - strand, within fixA at 43.236 kb on - strand at 43.236 kb on - strand at 43.237 kb on + strand at 43.237 kb on + strand at 43.270 kb on - strand at 43.318 kb on - strand, within fixB at 43.432 kb on + strand, within fixB at 43.506 kb on + strand, within fixB at 43.531 kb on + strand, within fixB at 43.539 kb on - strand, within fixB at 43.574 kb on - strand, within fixB at 43.610 kb on + strand, within fixB at 43.630 kb on - strand, within fixB at 43.630 kb on - strand, within fixB at 43.682 kb on - strand, within fixB at 43.692 kb on + strand, within fixB at 43.740 kb on + strand, within fixB at 43.740 kb on + strand, within fixB at 43.835 kb on + strand, within fixB at 43.835 kb on + strand, within fixB at 43.835 kb on - strand, within fixB at 43.838 kb on + strand, within fixB at 43.867 kb on + strand, within fixB at 43.867 kb on + strand, within fixB at 43.871 kb on - strand, within fixB at 43.924 kb on + strand, within fixB at 43.952 kb on + strand, within fixB at 43.970 kb on - strand, within fixB at 44.076 kb on + strand at 44.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0000015625 MOI TC1 remove 41,409 + caiT b0040 0.65 +2.4 41,411 + caiT b0040 0.66 -0.1 41,417 - caiT b0040 0.66 -0.4 41,417 - caiT b0040 0.66 -0.8 41,487 - caiT b0040 0.71 +0.3 41,574 - caiT b0040 0.76 -0.7 41,709 - caiT b0040 0.85 +0.4 41,709 - caiT b0040 0.85 -2.0 41,714 + caiT b0040 0.86 -0.6 41,716 + caiT b0040 0.86 +1.4 41,716 + caiT b0040 0.86 +0.2 41,723 - caiT b0040 0.86 +0.4 41,726 + caiT b0040 0.86 +0.4 41,726 + caiT b0040 0.86 -0.6 41,734 - caiT b0040 0.87 +1.5 41,734 - caiT b0040 0.87 +1.3 41,734 - caiT b0040 0.87 -0.2 41,734 - caiT b0040 0.87 +0.5 41,734 - caiT b0040 0.87 -1.0 41,734 - caiT b0040 0.87 -0.3 41,753 + caiT b0040 0.88 -0.7 41,753 + caiT b0040 0.88 +0.7 41,753 + caiT b0040 0.88 +2.4 41,753 + caiT b0040 0.88 +0.4 41,753 + caiT b0040 0.88 -1.1 41,753 + caiT b0040 0.88 +1.0 41,757 - caiT b0040 0.88 -0.5 41,757 - caiT b0040 0.88 -1.0 41,758 + caiT b0040 0.89 +1.4 41,759 - caiT b0040 0.89 +0.5 41,761 + caiT b0040 0.89 +0.9 41,761 + caiT b0040 0.89 +0.1 41,761 - caiT b0040 0.89 +1.0 41,761 - caiT b0040 0.89 -1.0 41,771 + caiT b0040 0.89 +0.7 41,771 + caiT b0040 0.89 +0.2 41,771 + caiT b0040 0.89 +0.4 41,771 + caiT b0040 0.89 +0.8 41,771 + caiT b0040 0.89 -0.6 41,774 + caiT b0040 0.90 +0.3 41,779 + caiT b0040 0.90 +0.6 41,783 + -2.5 41,786 - -0.4 41,791 - -1.5 41,791 - +0.6 41,794 - -1.7 41,795 + +0.2 41,797 + +1.1 41,803 - -1.5 41,830 + -0.2 41,830 + +0.0 41,830 + -0.1 41,838 - +0.4 41,838 - +0.4 41,844 - +0.9 41,846 - +0.6 41,849 + -1.0 41,850 + -0.9 41,938 + +0.4 42,028 + +2.4 42,028 + +2.4 42,028 + +2.4 42,040 + +0.4 42,041 + +2.4 42,041 + +2.4 42,041 + -0.7 42,041 + +2.4 42,043 + +2.4 42,048 - -0.3 42,049 - +0.1 42,059 - -0.7 42,070 - -0.7 42,070 - +0.3 42,070 - -0.2 42,070 - -0.4 42,097 + +0.6 42,098 + -0.6 42,098 + -0.2 42,430 - -1.5 42,442 - -1.6 42,446 + +0.9 42,447 - +1.0 42,452 - +0.0 42,452 - +0.1 42,456 + +0.4 42,464 - +0.4 42,464 - +0.0 42,466 - -0.8 42,466 - -2.2 42,466 - -1.1 42,466 - +2.4 42,466 - +3.4 42,470 + -0.6 42,478 - +1.3 42,478 - -0.3 42,478 - -0.2 42,485 + fixA b0041 0.11 +1.1 42,488 - fixA b0041 0.11 +0.6 42,491 - fixA b0041 0.11 +3.2 42,491 - fixA b0041 0.11 +0.4 42,534 - fixA b0041 0.17 +0.6 42,541 + fixA b0041 0.18 +0.3 42,550 - fixA b0041 0.19 +0.4 42,591 - fixA b0041 0.24 -0.4 42,601 - fixA b0041 0.26 +0.0 42,606 - fixA b0041 0.26 +0.1 42,682 - fixA b0041 0.36 +0.3 42,745 + fixA b0041 0.44 -0.1 42,873 - fixA b0041 0.61 +0.5 43,044 + fixA b0041 0.83 +0.1 43,058 + fixA b0041 0.85 -0.4 43,087 - fixA b0041 0.89 +0.9 43,236 - -0.2 43,236 - +0.4 43,237 + -0.0 43,237 + +0.5 43,270 - -0.9 43,318 - fixB b0042 0.14 -1.6 43,432 + fixB b0042 0.26 -0.3 43,506 + fixB b0042 0.34 +0.1 43,531 + fixB b0042 0.36 -0.5 43,539 - fixB b0042 0.37 -0.5 43,574 - fixB b0042 0.41 +0.3 43,610 + fixB b0042 0.45 +0.1 43,630 - fixB b0042 0.47 -0.3 43,630 - fixB b0042 0.47 +0.2 43,682 - fixB b0042 0.52 -0.2 43,692 + fixB b0042 0.54 -0.5 43,740 + fixB b0042 0.59 +0.5 43,740 + fixB b0042 0.59 +0.2 43,835 + fixB b0042 0.69 +1.0 43,835 + fixB b0042 0.69 -0.8 43,835 - fixB b0042 0.69 +0.6 43,838 + fixB b0042 0.69 -0.3 43,867 + fixB b0042 0.72 -0.6 43,867 + fixB b0042 0.72 -0.1 43,871 - fixB b0042 0.73 -0.6 43,924 + fixB b0042 0.78 -0.0 43,952 + fixB b0042 0.81 -0.1 43,970 - fixB b0042 0.83 -0.7 44,076 + -0.1 44,158 - -0.7
Or see this region's nucleotide sequence