Experiment: T7_phage 0.000015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbX and rfbC overlap by 4 nucleotides rfbC and rfbA overlap by 1 nucleotides rfbA and rfbD are separated by 57 nucleotides rfbD and rfbB overlap by 1 nucleotides
b2037: rfbX - predicted polisoprenol-linked O-antigen transporter (NCBI), at 2,106,361 to 2,107,608
rfbX
b2038: rfbC - dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI), at 2,107,605 to 2,108,162
rfbC
b2039: rfbA - glucose-1-phosphate thymidylyltransferase (NCBI), at 2,108,162 to 2,109,043
rfbA
b2040: rfbD - dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI), at 2,109,101 to 2,110,000
rfbD
b2041: rfbB - dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI), at 2,110,000 to 2,111,085
rfbB
Position (kb)
2108
2109
2110 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2107.198 kb on - strand, within rfbX at 2107.211 kb on - strand, within rfbX at 2107.222 kb on - strand, within rfbX at 2107.249 kb on - strand, within rfbX at 2107.257 kb on - strand, within rfbX at 2107.314 kb on - strand, within rfbX at 2107.314 kb on - strand, within rfbX at 2107.319 kb on - strand, within rfbX at 2107.400 kb on - strand, within rfbX at 2107.455 kb on - strand, within rfbX at 2107.458 kb on - strand, within rfbX at 2107.460 kb on - strand, within rfbX at 2107.505 kb on - strand at 2107.505 kb on - strand at 2107.519 kb on - strand at 2107.530 kb on - strand at 2107.530 kb on - strand at 2107.574 kb on - strand at 2107.574 kb on - strand at 2107.574 kb on - strand at 2107.575 kb on - strand at 2107.575 kb on - strand at 2107.577 kb on - strand at 2107.624 kb on - strand at 2107.632 kb on - strand at 2107.632 kb on - strand at 2107.633 kb on - strand at 2107.634 kb on - strand at 2107.649 kb on - strand at 2107.649 kb on - strand at 2107.692 kb on - strand, within rfbC at 2107.692 kb on - strand, within rfbC at 2107.717 kb on - strand, within rfbC at 2107.725 kb on - strand, within rfbC at 2107.801 kb on - strand, within rfbC at 2107.801 kb on - strand at 2107.804 kb on - strand, within rfbC at 2107.811 kb on - strand, within rfbC at 2107.825 kb on + strand, within rfbC at 2107.935 kb on - strand, within rfbC at 2107.938 kb on - strand, within rfbC at 2107.938 kb on - strand, within rfbC at 2107.940 kb on - strand, within rfbC at 2107.946 kb on - strand, within rfbC at 2107.946 kb on - strand, within rfbC at 2107.948 kb on - strand, within rfbC at 2107.948 kb on - strand, within rfbC at 2107.953 kb on - strand, within rfbC at 2107.977 kb on - strand, within rfbC at 2107.992 kb on - strand, within rfbC at 2108.015 kb on - strand, within rfbC at 2108.016 kb on - strand, within rfbC at 2108.024 kb on - strand, within rfbC at 2108.027 kb on - strand, within rfbC at 2108.027 kb on - strand, within rfbC at 2108.128 kb on - strand at 2108.286 kb on - strand, within rfbA at 2108.286 kb on - strand, within rfbA at 2108.294 kb on - strand, within rfbA at 2108.300 kb on - strand, within rfbA at 2108.309 kb on - strand, within rfbA at 2108.309 kb on - strand, within rfbA at 2108.317 kb on - strand, within rfbA at 2108.317 kb on - strand, within rfbA at 2108.345 kb on - strand, within rfbA at 2108.345 kb on - strand, within rfbA at 2108.349 kb on - strand, within rfbA at 2108.349 kb on - strand, within rfbA at 2108.361 kb on - strand, within rfbA at 2108.361 kb on - strand, within rfbA at 2108.364 kb on + strand, within rfbA at 2108.366 kb on - strand, within rfbA at 2108.366 kb on - strand, within rfbA at 2108.366 kb on - strand, within rfbA at 2108.383 kb on - strand, within rfbA at 2108.383 kb on - strand, within rfbA at 2108.383 kb on - strand, within rfbA at 2108.395 kb on - strand, within rfbA at 2108.395 kb on - strand, within rfbA at 2108.397 kb on - strand, within rfbA at 2108.397 kb on - strand, within rfbA at 2108.429 kb on - strand, within rfbA at 2108.459 kb on - strand, within rfbA at 2108.463 kb on - strand, within rfbA at 2108.478 kb on - strand, within rfbA at 2108.485 kb on - strand, within rfbA at 2108.494 kb on - strand, within rfbA at 2108.494 kb on - strand, within rfbA at 2108.521 kb on + strand, within rfbA at 2108.521 kb on - strand, within rfbA at 2108.529 kb on - strand, within rfbA at 2108.564 kb on - strand, within rfbA at 2108.564 kb on - strand, within rfbA at 2108.568 kb on - strand, within rfbA at 2108.604 kb on + strand, within rfbA at 2108.610 kb on - strand, within rfbA at 2108.612 kb on - strand, within rfbA at 2108.629 kb on - strand, within rfbA at 2108.641 kb on - strand, within rfbA at 2108.641 kb on - strand, within rfbA at 2108.682 kb on - strand, within rfbA at 2108.682 kb on - strand, within rfbA at 2108.682 kb on - strand, within rfbA at 2108.682 kb on - strand, within rfbA at 2108.718 kb on - strand, within rfbA at 2108.728 kb on - strand, within rfbA at 2108.728 kb on - strand, within rfbA at 2108.732 kb on + strand, within rfbA at 2108.734 kb on + strand, within rfbA at 2108.771 kb on - strand, within rfbA at 2108.790 kb on - strand, within rfbA at 2108.795 kb on - strand, within rfbA at 2108.807 kb on - strand, within rfbA at 2108.812 kb on + strand, within rfbA at 2108.814 kb on - strand, within rfbA at 2108.815 kb on - strand, within rfbA at 2108.815 kb on - strand, within rfbA at 2108.828 kb on - strand, within rfbA at 2108.828 kb on - strand, within rfbA at 2108.830 kb on - strand, within rfbA at 2108.832 kb on - strand, within rfbA at 2108.834 kb on - strand, within rfbA at 2108.835 kb on - strand, within rfbA at 2108.835 kb on - strand, within rfbA at 2108.835 kb on - strand, within rfbA at 2108.835 kb on - strand, within rfbA at 2108.835 kb on - strand, within rfbA at 2108.853 kb on - strand, within rfbA at 2108.864 kb on + strand, within rfbA at 2108.864 kb on + strand, within rfbA at 2108.864 kb on + strand, within rfbA at 2108.864 kb on + strand, within rfbA at 2108.870 kb on - strand, within rfbA at 2108.870 kb on - strand, within rfbA at 2108.874 kb on + strand, within rfbA at 2108.874 kb on + strand, within rfbA at 2108.913 kb on + strand, within rfbA at 2108.913 kb on + strand, within rfbA at 2108.922 kb on - strand, within rfbA at 2108.922 kb on - strand, within rfbA at 2108.924 kb on - strand, within rfbA at 2108.924 kb on - strand, within rfbA at 2108.926 kb on + strand, within rfbA at 2108.953 kb on - strand, within rfbA at 2108.956 kb on - strand at 2108.957 kb on - strand at 2108.973 kb on - strand at 2108.977 kb on - strand at 2108.977 kb on - strand at 2108.982 kb on - strand at 2108.982 kb on - strand at 2108.996 kb on - strand at 2108.996 kb on - strand at 2108.996 kb on - strand at 2108.996 kb on - strand at 2108.998 kb on - strand at 2109.009 kb on - strand at 2109.076 kb on - strand at 2109.148 kb on - strand at 2109.148 kb on - strand at 2109.148 kb on - strand at 2109.159 kb on - strand at 2109.159 kb on - strand at 2109.166 kb on - strand at 2109.234 kb on + strand, within rfbD at 2109.236 kb on + strand, within rfbD at 2109.239 kb on - strand at 2109.239 kb on - strand, within rfbD at 2109.240 kb on - strand, within rfbD at 2109.253 kb on + strand, within rfbD at 2109.253 kb on + strand, within rfbD at 2109.254 kb on - strand, within rfbD at 2109.266 kb on - strand, within rfbD at 2109.272 kb on - strand, within rfbD at 2109.274 kb on + strand, within rfbD at 2109.299 kb on - strand, within rfbD at 2109.299 kb on - strand, within rfbD at 2109.329 kb on - strand, within rfbD at 2109.329 kb on - strand, within rfbD at 2109.329 kb on - strand, within rfbD at 2109.336 kb on + strand, within rfbD at 2109.344 kb on - strand, within rfbD at 2109.353 kb on - strand, within rfbD at 2109.360 kb on - strand, within rfbD at 2109.362 kb on + strand, within rfbD at 2109.362 kb on + strand, within rfbD at 2109.370 kb on - strand, within rfbD at 2109.370 kb on - strand, within rfbD at 2109.370 kb on - strand, within rfbD at 2109.370 kb on - strand, within rfbD at 2109.370 kb on - strand, within rfbD at 2109.375 kb on - strand, within rfbD at 2109.409 kb on + strand, within rfbD at 2109.412 kb on - strand, within rfbD at 2109.412 kb on - strand, within rfbD at 2109.412 kb on - strand, within rfbD at 2109.412 kb on - strand, within rfbD at 2109.421 kb on + strand, within rfbD at 2109.430 kb on + strand, within rfbD at 2109.438 kb on - strand, within rfbD at 2109.438 kb on - strand, within rfbD at 2109.521 kb on + strand, within rfbD at 2109.521 kb on + strand, within rfbD at 2109.653 kb on - strand, within rfbD at 2109.653 kb on - strand, within rfbD at 2109.653 kb on - strand, within rfbD at 2109.698 kb on - strand, within rfbD at 2109.726 kb on - strand, within rfbD at 2109.742 kb on + strand, within rfbD at 2109.742 kb on + strand, within rfbD at 2109.742 kb on + strand, within rfbD at 2109.767 kb on - strand, within rfbD at 2109.797 kb on + strand, within rfbD at 2109.797 kb on + strand, within rfbD at 2109.805 kb on - strand, within rfbD at 2109.805 kb on - strand, within rfbD at 2109.805 kb on - strand, within rfbD at 2109.805 kb on - strand, within rfbD at 2109.805 kb on - strand, within rfbD at 2109.815 kb on - strand, within rfbD at 2109.815 kb on - strand, within rfbD at 2109.861 kb on + strand, within rfbD at 2109.908 kb on - strand, within rfbD at 2109.936 kb on + strand at 2109.936 kb on + strand at 2109.943 kb on + strand at 2109.945 kb on + strand at 2109.945 kb on - strand at 2109.947 kb on + strand at 2109.958 kb on - strand at 2109.958 kb on - strand at 2109.958 kb on - strand at 2109.960 kb on - strand at 2109.963 kb on - strand at 2109.963 kb on - strand at 2109.963 kb on - strand at 2109.963 kb on - strand at 2109.965 kb on + strand at 2109.965 kb on - strand at 2109.995 kb on + strand at 2110.018 kb on - strand at 2110.036 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.000015625 MOI TC1 remove 2,107,198 - rfbX b2037 0.67 -0.6 2,107,211 - rfbX b2037 0.68 +0.2 2,107,222 - rfbX b2037 0.69 -0.6 2,107,249 - rfbX b2037 0.71 -2.2 2,107,257 - rfbX b2037 0.72 +0.7 2,107,314 - rfbX b2037 0.76 -1.1 2,107,314 - rfbX b2037 0.76 +3.5 2,107,319 - rfbX b2037 0.77 -0.5 2,107,400 - rfbX b2037 0.83 +0.8 2,107,455 - rfbX b2037 0.88 -1.1 2,107,458 - rfbX b2037 0.88 -0.1 2,107,460 - rfbX b2037 0.88 -0.6 2,107,505 - -1.0 2,107,505 - +1.5 2,107,519 - -1.5 2,107,530 - +1.7 2,107,530 - -1.3 2,107,574 - +0.9 2,107,574 - -2.3 2,107,574 - -0.5 2,107,575 - +0.6 2,107,575 - +0.7 2,107,577 - -1.0 2,107,624 - +0.5 2,107,632 - -3.5 2,107,632 - -3.0 2,107,633 - +0.1 2,107,634 - -3.6 2,107,649 - -2.8 2,107,649 - -1.3 2,107,692 - rfbC b2038 0.16 -4.8 2,107,692 - rfbC b2038 0.16 -3.2 2,107,717 - rfbC b2038 0.20 -2.8 2,107,725 - rfbC b2038 0.22 -3.4 2,107,801 - rfbC b2038 0.35 -1.6 2,107,801 - -0.3 2,107,804 - rfbC b2038 0.36 -4.3 2,107,811 - rfbC b2038 0.37 -3.7 2,107,825 + rfbC b2038 0.39 -0.3 2,107,935 - rfbC b2038 0.59 -1.1 2,107,938 - rfbC b2038 0.60 -3.8 2,107,938 - rfbC b2038 0.60 -1.8 2,107,940 - rfbC b2038 0.60 -3.7 2,107,946 - rfbC b2038 0.61 -2.7 2,107,946 - rfbC b2038 0.61 -3.8 2,107,948 - rfbC b2038 0.61 -5.6 2,107,948 - rfbC b2038 0.61 -3.5 2,107,953 - rfbC b2038 0.62 -3.9 2,107,977 - rfbC b2038 0.67 -3.1 2,107,992 - rfbC b2038 0.69 -4.3 2,108,015 - rfbC b2038 0.73 -2.0 2,108,016 - rfbC b2038 0.74 -1.1 2,108,024 - rfbC b2038 0.75 -3.1 2,108,027 - rfbC b2038 0.76 -0.6 2,108,027 - rfbC b2038 0.76 -2.2 2,108,128 - -1.6 2,108,286 - rfbA b2039 0.14 -0.5 2,108,286 - rfbA b2039 0.14 -0.1 2,108,294 - rfbA b2039 0.15 -0.4 2,108,300 - rfbA b2039 0.16 -0.6 2,108,309 - rfbA b2039 0.17 -0.4 2,108,309 - rfbA b2039 0.17 -0.1 2,108,317 - rfbA b2039 0.18 +0.2 2,108,317 - rfbA b2039 0.18 -0.6 2,108,345 - rfbA b2039 0.21 +1.8 2,108,345 - rfbA b2039 0.21 +0.7 2,108,349 - rfbA b2039 0.21 +0.2 2,108,349 - rfbA b2039 0.21 -0.0 2,108,361 - rfbA b2039 0.23 -0.0 2,108,361 - rfbA b2039 0.23 -0.4 2,108,364 + rfbA b2039 0.23 +1.7 2,108,366 - rfbA b2039 0.23 -0.2 2,108,366 - rfbA b2039 0.23 +1.0 2,108,366 - rfbA b2039 0.23 +0.5 2,108,383 - rfbA b2039 0.25 -1.2 2,108,383 - rfbA b2039 0.25 -1.3 2,108,383 - rfbA b2039 0.25 +1.0 2,108,395 - rfbA b2039 0.26 +0.2 2,108,395 - rfbA b2039 0.26 +0.7 2,108,397 - rfbA b2039 0.27 -1.5 2,108,397 - rfbA b2039 0.27 -0.8 2,108,429 - rfbA b2039 0.30 +1.4 2,108,459 - rfbA b2039 0.34 +0.9 2,108,463 - rfbA b2039 0.34 +0.7 2,108,478 - rfbA b2039 0.36 +0.2 2,108,485 - rfbA b2039 0.37 -0.2 2,108,494 - rfbA b2039 0.38 +1.7 2,108,494 - rfbA b2039 0.38 -0.6 2,108,521 + rfbA b2039 0.41 +1.7 2,108,521 - rfbA b2039 0.41 -0.6 2,108,529 - rfbA b2039 0.42 -0.1 2,108,564 - rfbA b2039 0.46 +1.4 2,108,564 - rfbA b2039 0.46 -0.2 2,108,568 - rfbA b2039 0.46 +0.5 2,108,604 + rfbA b2039 0.50 -0.9 2,108,610 - rfbA b2039 0.51 -0.1 2,108,612 - rfbA b2039 0.51 -0.1 2,108,629 - rfbA b2039 0.53 -1.5 2,108,641 - rfbA b2039 0.54 +0.9 2,108,641 - rfbA b2039 0.54 -0.8 2,108,682 - rfbA b2039 0.59 +0.2 2,108,682 - rfbA b2039 0.59 +0.5 2,108,682 - rfbA b2039 0.59 -0.9 2,108,682 - rfbA b2039 0.59 +0.2 2,108,718 - rfbA b2039 0.63 -0.0 2,108,728 - rfbA b2039 0.64 -0.7 2,108,728 - rfbA b2039 0.64 +1.3 2,108,732 + rfbA b2039 0.65 +3.3 2,108,734 + rfbA b2039 0.65 +3.3 2,108,771 - rfbA b2039 0.69 -0.7 2,108,790 - rfbA b2039 0.71 -1.9 2,108,795 - rfbA b2039 0.72 +0.0 2,108,807 - rfbA b2039 0.73 +0.5 2,108,812 + rfbA b2039 0.74 +0.7 2,108,814 - rfbA b2039 0.74 +1.1 2,108,815 - rfbA b2039 0.74 -0.0 2,108,815 - rfbA b2039 0.74 -0.7 2,108,828 - rfbA b2039 0.76 -1.3 2,108,828 - rfbA b2039 0.76 -0.6 2,108,830 - rfbA b2039 0.76 -0.5 2,108,832 - rfbA b2039 0.76 -0.1 2,108,834 - rfbA b2039 0.76 -2.2 2,108,835 - rfbA b2039 0.76 -0.6 2,108,835 - rfbA b2039 0.76 -0.3 2,108,835 - rfbA b2039 0.76 +0.8 2,108,835 - rfbA b2039 0.76 +2.1 2,108,835 - rfbA b2039 0.76 -0.4 2,108,853 - rfbA b2039 0.78 -0.0 2,108,864 + rfbA b2039 0.80 +0.4 2,108,864 + rfbA b2039 0.80 +1.5 2,108,864 + rfbA b2039 0.80 -0.5 2,108,864 + rfbA b2039 0.80 -0.1 2,108,870 - rfbA b2039 0.80 -0.4 2,108,870 - rfbA b2039 0.80 -0.1 2,108,874 + rfbA b2039 0.81 -3.0 2,108,874 + rfbA b2039 0.81 +0.3 2,108,913 + rfbA b2039 0.85 +0.1 2,108,913 + rfbA b2039 0.85 +1.7 2,108,922 - rfbA b2039 0.86 -1.8 2,108,922 - rfbA b2039 0.86 -0.2 2,108,924 - rfbA b2039 0.86 +0.1 2,108,924 - rfbA b2039 0.86 -1.2 2,108,926 + rfbA b2039 0.87 +0.7 2,108,953 - rfbA b2039 0.90 +0.5 2,108,956 - +0.9 2,108,957 - -0.2 2,108,973 - +0.6 2,108,977 - +0.2 2,108,977 - -0.1 2,108,982 - +0.6 2,108,982 - -0.5 2,108,996 - +1.7 2,108,996 - -0.1 2,108,996 - -0.2 2,108,996 - +0.3 2,108,998 - +1.6 2,109,009 - +0.6 2,109,076 - -0.0 2,109,148 - -1.8 2,109,148 - -2.4 2,109,148 - -4.7 2,109,159 - -2.6 2,109,159 - -3.8 2,109,166 - -2.7 2,109,234 + rfbD b2040 0.15 +0.1 2,109,236 + rfbD b2040 0.15 +1.7 2,109,239 - -2.5 2,109,239 - rfbD b2040 0.15 -2.1 2,109,240 - rfbD b2040 0.15 -5.5 2,109,253 + rfbD b2040 0.17 +1.7 2,109,253 + rfbD b2040 0.17 +1.7 2,109,254 - rfbD b2040 0.17 -3.9 2,109,266 - rfbD b2040 0.18 -4.5 2,109,272 - rfbD b2040 0.19 -4.1 2,109,274 + rfbD b2040 0.19 +1.7 2,109,299 - rfbD b2040 0.22 -3.2 2,109,299 - rfbD b2040 0.22 -3.4 2,109,329 - rfbD b2040 0.25 -4.3 2,109,329 - rfbD b2040 0.25 -0.9 2,109,329 - rfbD b2040 0.25 -4.0 2,109,336 + rfbD b2040 0.26 -1.1 2,109,344 - rfbD b2040 0.27 -2.6 2,109,353 - rfbD b2040 0.28 -2.8 2,109,360 - rfbD b2040 0.29 -4.3 2,109,362 + rfbD b2040 0.29 -1.3 2,109,362 + rfbD b2040 0.29 -0.3 2,109,370 - rfbD b2040 0.30 -4.1 2,109,370 - rfbD b2040 0.30 -4.3 2,109,370 - rfbD b2040 0.30 -4.4 2,109,370 - rfbD b2040 0.30 -4.3 2,109,370 - rfbD b2040 0.30 -4.6 2,109,375 - rfbD b2040 0.30 -3.9 2,109,409 + rfbD b2040 0.34 -2.7 2,109,412 - rfbD b2040 0.35 -3.1 2,109,412 - rfbD b2040 0.35 -1.1 2,109,412 - rfbD b2040 0.35 -1.9 2,109,412 - rfbD b2040 0.35 -3.8 2,109,421 + rfbD b2040 0.36 -1.5 2,109,430 + rfbD b2040 0.37 -1.9 2,109,438 - rfbD b2040 0.37 -3.0 2,109,438 - rfbD b2040 0.37 -5.1 2,109,521 + rfbD b2040 0.47 -1.5 2,109,521 + rfbD b2040 0.47 +1.7 2,109,653 - rfbD b2040 0.61 -3.1 2,109,653 - rfbD b2040 0.61 -4.6 2,109,653 - rfbD b2040 0.61 -3.2 2,109,698 - rfbD b2040 0.66 -2.8 2,109,726 - rfbD b2040 0.69 -5.6 2,109,742 + rfbD b2040 0.71 -3.3 2,109,742 + rfbD b2040 0.71 -0.9 2,109,742 + rfbD b2040 0.71 -2.3 2,109,767 - rfbD b2040 0.74 -5.1 2,109,797 + rfbD b2040 0.77 +0.1 2,109,797 + rfbD b2040 0.77 +1.7 2,109,805 - rfbD b2040 0.78 -2.9 2,109,805 - rfbD b2040 0.78 -5.1 2,109,805 - rfbD b2040 0.78 -2.0 2,109,805 - rfbD b2040 0.78 -2.1 2,109,805 - rfbD b2040 0.78 -3.3 2,109,815 - rfbD b2040 0.79 -3.6 2,109,815 - rfbD b2040 0.79 -3.0 2,109,861 + rfbD b2040 0.84 +1.7 2,109,908 - rfbD b2040 0.90 -4.5 2,109,936 + -0.9 2,109,936 + +1.7 2,109,943 + +0.7 2,109,945 + +0.1 2,109,945 - -3.9 2,109,947 + -1.3 2,109,958 - -3.8 2,109,958 - -3.5 2,109,958 - -4.5 2,109,960 - -3.5 2,109,963 - -3.5 2,109,963 - -4.8 2,109,963 - -1.9 2,109,963 - -4.5 2,109,965 + +0.7 2,109,965 - -3.6 2,109,995 + +0.7 2,110,018 - -0.3 2,110,036 - -0.2
Or see this region's nucleotide sequence