Experiment: T7_phage 0.0015625 MOI TC1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yidP and yidE overlap by 4 nucleotides yidE and ibpB are separated by 195 nucleotides ibpB and ibpA are separated by 111 nucleotides
b3684: yidP - predicted DNA-binding transcriptional regulator (NCBI), at 3,861,922 to 3,862,638
yidP
b3685: yidE - putative transport protein (VIMSS), at 3,862,635 to 3,864,296
yidE
b3686: ibpB - heat shock chaperone (NCBI), at 3,864,492 to 3,864,920
ibpB
b3687: ibpA - heat shock chaperone (NCBI), at 3,865,032 to 3,865,445
ibpA
Position (kb)
3862
3863
3864
3865 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3861.701 kb on + strand at 3861.770 kb on + strand at 3861.805 kb on + strand at 3861.805 kb on + strand at 3861.821 kb on - strand at 3861.823 kb on - strand at 3861.883 kb on + strand at 3862.043 kb on - strand, within yidP at 3862.065 kb on + strand, within yidP at 3862.074 kb on - strand, within yidP at 3862.098 kb on + strand, within yidP at 3862.251 kb on + strand, within yidP at 3862.369 kb on + strand, within yidP at 3862.375 kb on + strand, within yidP at 3862.375 kb on + strand, within yidP at 3862.393 kb on - strand, within yidP at 3862.393 kb on - strand, within yidP at 3862.399 kb on - strand, within yidP at 3862.403 kb on + strand, within yidP at 3862.443 kb on - strand, within yidP at 3862.454 kb on - strand, within yidP at 3862.532 kb on + strand, within yidP at 3862.532 kb on + strand, within yidP at 3862.538 kb on - strand, within yidP at 3862.538 kb on - strand, within yidP at 3862.538 kb on - strand, within yidP at 3862.572 kb on + strand at 3862.572 kb on + strand at 3862.591 kb on + strand at 3862.600 kb on - strand at 3862.625 kb on - strand at 3862.625 kb on - strand at 3862.673 kb on - strand at 3862.794 kb on + strand at 3862.802 kb on - strand, within yidE at 3862.856 kb on - strand, within yidE at 3862.881 kb on - strand, within yidE at 3862.887 kb on + strand, within yidE at 3862.891 kb on - strand, within yidE at 3862.891 kb on - strand, within yidE at 3862.912 kb on + strand, within yidE at 3862.979 kb on - strand, within yidE at 3862.981 kb on + strand, within yidE at 3863.016 kb on - strand, within yidE at 3863.031 kb on - strand, within yidE at 3863.048 kb on + strand, within yidE at 3863.048 kb on + strand, within yidE at 3863.118 kb on - strand, within yidE at 3863.143 kb on - strand, within yidE at 3863.143 kb on - strand, within yidE at 3863.160 kb on + strand, within yidE at 3863.162 kb on - strand, within yidE at 3863.162 kb on - strand, within yidE at 3863.217 kb on + strand, within yidE at 3863.217 kb on + strand, within yidE at 3863.218 kb on - strand, within yidE at 3863.225 kb on + strand, within yidE at 3863.262 kb on - strand, within yidE at 3863.300 kb on - strand, within yidE at 3863.302 kb on - strand, within yidE at 3863.328 kb on + strand, within yidE at 3863.328 kb on + strand, within yidE at 3863.336 kb on - strand, within yidE at 3863.336 kb on - strand, within yidE at 3863.336 kb on - strand, within yidE at 3863.339 kb on + strand, within yidE at 3863.339 kb on + strand, within yidE at 3863.347 kb on - strand, within yidE at 3863.348 kb on - strand, within yidE at 3863.411 kb on + strand, within yidE at 3863.414 kb on - strand, within yidE at 3863.425 kb on - strand, within yidE at 3863.425 kb on - strand, within yidE at 3863.435 kb on - strand, within yidE at 3863.435 kb on - strand, within yidE at 3863.517 kb on - strand, within yidE at 3863.522 kb on - strand, within yidE at 3863.523 kb on - strand, within yidE at 3863.545 kb on + strand, within yidE at 3863.583 kb on + strand, within yidE at 3863.583 kb on + strand, within yidE at 3863.589 kb on - strand, within yidE at 3863.636 kb on + strand, within yidE at 3863.636 kb on + strand, within yidE at 3863.651 kb on + strand, within yidE at 3863.651 kb on + strand, within yidE at 3863.659 kb on - strand, within yidE at 3863.712 kb on - strand, within yidE at 3863.721 kb on + strand, within yidE at 3863.774 kb on - strand, within yidE at 3863.774 kb on - strand, within yidE at 3863.776 kb on + strand, within yidE at 3863.815 kb on + strand, within yidE at 3863.815 kb on + strand, within yidE at 3863.832 kb on - strand, within yidE at 3863.832 kb on - strand, within yidE at 3863.859 kb on - strand, within yidE at 3863.859 kb on - strand, within yidE at 3863.894 kb on - strand at 3863.899 kb on + strand, within yidE at 3863.899 kb on + strand, within yidE at 3863.909 kb on - strand, within yidE at 3863.955 kb on + strand, within yidE at 3864.007 kb on + strand, within yidE at 3864.007 kb on + strand, within yidE at 3864.007 kb on + strand, within yidE at 3864.039 kb on + strand, within yidE at 3864.053 kb on - strand, within yidE at 3864.059 kb on + strand, within yidE at 3864.087 kb on - strand, within yidE at 3864.130 kb on - strand at 3864.140 kb on - strand at 3864.140 kb on - strand at 3864.170 kb on + strand at 3864.186 kb on - strand at 3864.186 kb on - strand at 3864.201 kb on + strand at 3864.214 kb on - strand at 3864.214 kb on - strand at 3864.239 kb on + strand at 3864.239 kb on + strand at 3864.269 kb on - strand at 3864.275 kb on + strand at 3864.283 kb on - strand at 3864.284 kb on + strand at 3864.289 kb on - strand at 3864.292 kb on - strand at 3864.292 kb on - strand at 3864.295 kb on + strand at 3864.376 kb on + strand at 3864.433 kb on + strand at 3864.488 kb on - strand at 3864.488 kb on - strand at 3864.510 kb on + strand at 3864.545 kb on + strand, within ibpB at 3864.569 kb on - strand, within ibpB at 3864.583 kb on - strand, within ibpB at 3864.626 kb on + strand, within ibpB at 3864.719 kb on + strand, within ibpB at 3864.719 kb on + strand, within ibpB at 3864.727 kb on - strand, within ibpB at 3864.727 kb on - strand, within ibpB at 3864.733 kb on + strand, within ibpB at 3864.778 kb on - strand, within ibpB at 3864.778 kb on - strand, within ibpB at 3864.778 kb on - strand, within ibpB at 3864.789 kb on - strand, within ibpB at 3864.812 kb on - strand, within ibpB at 3864.856 kb on + strand, within ibpB at 3864.864 kb on - strand, within ibpB at 3864.866 kb on + strand, within ibpB at 3864.870 kb on + strand, within ibpB at 3865.029 kb on - strand at 3865.029 kb on - strand at 3865.029 kb on - strand at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.098 kb on - strand, within ibpA at 3865.098 kb on - strand, within ibpA at 3865.098 kb on - strand, within ibpA at 3865.112 kb on + strand, within ibpA at 3865.113 kb on - strand, within ibpA at 3865.120 kb on - strand, within ibpA at 3865.120 kb on - strand, within ibpA at 3865.120 kb on - strand, within ibpA at 3865.180 kb on + strand, within ibpA at 3865.182 kb on - strand, within ibpA at 3865.184 kb on + strand, within ibpA at 3865.210 kb on - strand, within ibpA at 3865.217 kb on + strand, within ibpA at 3865.233 kb on - strand, within ibpA at 3865.233 kb on - strand, within ibpA at 3865.240 kb on + strand, within ibpA at 3865.241 kb on + strand, within ibpA at 3865.244 kb on - strand, within ibpA at 3865.244 kb on - strand, within ibpA at 3865.253 kb on + strand, within ibpA at 3865.253 kb on - strand, within ibpA
Per-strain Table
Position Strand Gene LocusTag Fraction T7_phage 0.0015625 MOI TC1 remove 3,861,701 + +1.6 3,861,770 + +1.9 3,861,805 + -0.6 3,861,805 + -0.8 3,861,821 - -0.5 3,861,823 - +0.9 3,861,883 + +1.7 3,862,043 - yidP b3684 0.17 -0.1 3,862,065 + yidP b3684 0.20 -0.5 3,862,074 - yidP b3684 0.21 -0.6 3,862,098 + yidP b3684 0.25 +0.2 3,862,251 + yidP b3684 0.46 +1.8 3,862,369 + yidP b3684 0.62 -0.1 3,862,375 + yidP b3684 0.63 -0.2 3,862,375 + yidP b3684 0.63 -1.7 3,862,393 - yidP b3684 0.66 +0.4 3,862,393 - yidP b3684 0.66 +0.3 3,862,399 - yidP b3684 0.67 +2.4 3,862,403 + yidP b3684 0.67 +0.3 3,862,443 - yidP b3684 0.73 +0.4 3,862,454 - yidP b3684 0.74 +0.2 3,862,532 + yidP b3684 0.85 +1.5 3,862,532 + yidP b3684 0.85 +1.5 3,862,538 - yidP b3684 0.86 +0.4 3,862,538 - yidP b3684 0.86 +0.1 3,862,538 - yidP b3684 0.86 -0.8 3,862,572 + -0.8 3,862,572 + +0.3 3,862,591 + +0.4 3,862,600 - -1.2 3,862,625 - +0.5 3,862,625 - +0.2 3,862,673 - +0.8 3,862,794 + -0.5 3,862,802 - yidE b3685 0.10 -0.5 3,862,856 - yidE b3685 0.13 +0.1 3,862,881 - yidE b3685 0.15 +1.1 3,862,887 + yidE b3685 0.15 -0.3 3,862,891 - yidE b3685 0.15 +0.3 3,862,891 - yidE b3685 0.15 +0.1 3,862,912 + yidE b3685 0.17 +0.0 3,862,979 - yidE b3685 0.21 +1.2 3,862,981 + yidE b3685 0.21 +2.3 3,863,016 - yidE b3685 0.23 -0.0 3,863,031 - yidE b3685 0.24 +0.9 3,863,048 + yidE b3685 0.25 -1.8 3,863,048 + yidE b3685 0.25 -0.7 3,863,118 - yidE b3685 0.29 -0.7 3,863,143 - yidE b3685 0.31 -0.1 3,863,143 - yidE b3685 0.31 -0.6 3,863,160 + yidE b3685 0.32 +0.8 3,863,162 - yidE b3685 0.32 +0.2 3,863,162 - yidE b3685 0.32 +2.4 3,863,217 + yidE b3685 0.35 +0.0 3,863,217 + yidE b3685 0.35 +0.0 3,863,218 - yidE b3685 0.35 +0.6 3,863,225 + yidE b3685 0.35 -1.0 3,863,262 - yidE b3685 0.38 +1.8 3,863,300 - yidE b3685 0.40 +1.7 3,863,302 - yidE b3685 0.40 -0.1 3,863,328 + yidE b3685 0.42 +2.0 3,863,328 + yidE b3685 0.42 +2.2 3,863,336 - yidE b3685 0.42 +0.3 3,863,336 - yidE b3685 0.42 -1.3 3,863,336 - yidE b3685 0.42 +2.3 3,863,339 + yidE b3685 0.42 +0.3 3,863,339 + yidE b3685 0.42 -0.5 3,863,347 - yidE b3685 0.43 +1.6 3,863,348 - yidE b3685 0.43 -0.4 3,863,411 + yidE b3685 0.47 -1.3 3,863,414 - yidE b3685 0.47 -0.7 3,863,425 - yidE b3685 0.48 +0.8 3,863,425 - yidE b3685 0.48 -0.7 3,863,435 - yidE b3685 0.48 +1.2 3,863,435 - yidE b3685 0.48 -0.9 3,863,517 - yidE b3685 0.53 -0.0 3,863,522 - yidE b3685 0.53 -0.4 3,863,523 - yidE b3685 0.53 -0.9 3,863,545 + yidE b3685 0.55 -0.2 3,863,583 + yidE b3685 0.57 +1.6 3,863,583 + yidE b3685 0.57 +0.0 3,863,589 - yidE b3685 0.57 +1.1 3,863,636 + yidE b3685 0.60 +1.0 3,863,636 + yidE b3685 0.60 -0.2 3,863,651 + yidE b3685 0.61 +3.6 3,863,651 + yidE b3685 0.61 +4.0 3,863,659 - yidE b3685 0.62 +0.5 3,863,712 - yidE b3685 0.65 +1.5 3,863,721 + yidE b3685 0.65 -1.5 3,863,774 - yidE b3685 0.69 -0.5 3,863,774 - yidE b3685 0.69 -0.8 3,863,776 + yidE b3685 0.69 -0.5 3,863,815 + yidE b3685 0.71 -0.7 3,863,815 + yidE b3685 0.71 +0.2 3,863,832 - yidE b3685 0.72 -0.3 3,863,832 - yidE b3685 0.72 -0.0 3,863,859 - yidE b3685 0.74 +0.2 3,863,859 - yidE b3685 0.74 -0.2 3,863,894 - +2.2 3,863,899 + yidE b3685 0.76 +1.0 3,863,899 + yidE b3685 0.76 +0.3 3,863,909 - yidE b3685 0.77 +2.6 3,863,955 + yidE b3685 0.79 -0.1 3,864,007 + yidE b3685 0.83 +0.7 3,864,007 + yidE b3685 0.83 +1.1 3,864,007 + yidE b3685 0.83 +0.1 3,864,039 + yidE b3685 0.84 -0.8 3,864,053 - yidE b3685 0.85 +1.7 3,864,059 + yidE b3685 0.86 +1.6 3,864,087 - yidE b3685 0.87 -0.1 3,864,130 - -0.0 3,864,140 - -0.2 3,864,140 - -0.7 3,864,170 + -1.2 3,864,186 - +1.1 3,864,186 - +1.1 3,864,201 + -1.4 3,864,214 - -0.5 3,864,214 - -0.8 3,864,239 + -0.3 3,864,239 + +0.1 3,864,269 - +0.0 3,864,275 + -1.3 3,864,283 - +0.2 3,864,284 + -0.4 3,864,289 - -0.5 3,864,292 - +0.9 3,864,292 - -0.9 3,864,295 + -0.3 3,864,376 + -0.2 3,864,433 + +2.3 3,864,488 - +1.1 3,864,488 - +0.2 3,864,510 + -0.6 3,864,545 + ibpB b3686 0.12 -0.1 3,864,569 - ibpB b3686 0.18 +0.1 3,864,583 - ibpB b3686 0.21 +2.1 3,864,626 + ibpB b3686 0.31 -1.8 3,864,719 + ibpB b3686 0.53 +0.8 3,864,719 + ibpB b3686 0.53 +0.2 3,864,727 - ibpB b3686 0.55 -0.7 3,864,727 - ibpB b3686 0.55 +0.1 3,864,733 + ibpB b3686 0.56 -0.4 3,864,778 - ibpB b3686 0.67 -0.3 3,864,778 - ibpB b3686 0.67 -0.6 3,864,778 - ibpB b3686 0.67 +0.3 3,864,789 - ibpB b3686 0.69 +0.4 3,864,812 - ibpB b3686 0.75 -3.1 3,864,856 + ibpB b3686 0.85 +1.7 3,864,864 - ibpB b3686 0.87 -0.1 3,864,866 + ibpB b3686 0.87 -0.2 3,864,870 + ibpB b3686 0.88 -0.6 3,865,029 - +0.3 3,865,029 - -0.6 3,865,029 - -0.1 3,865,090 + ibpA b3687 0.14 -0.1 3,865,090 + ibpA b3687 0.14 -0.3 3,865,090 + ibpA b3687 0.14 -0.8 3,865,090 + ibpA b3687 0.14 +0.2 3,865,090 + ibpA b3687 0.14 -1.0 3,865,098 - ibpA b3687 0.16 -1.5 3,865,098 - ibpA b3687 0.16 +0.5 3,865,098 - ibpA b3687 0.16 +1.1 3,865,112 + ibpA b3687 0.19 -0.8 3,865,113 - ibpA b3687 0.20 -1.1 3,865,120 - ibpA b3687 0.21 +0.3 3,865,120 - ibpA b3687 0.21 +0.1 3,865,120 - ibpA b3687 0.21 -3.3 3,865,180 + ibpA b3687 0.36 +0.6 3,865,182 - ibpA b3687 0.36 -2.8 3,865,184 + ibpA b3687 0.37 -0.6 3,865,210 - ibpA b3687 0.43 +1.6 3,865,217 + ibpA b3687 0.45 -2.2 3,865,233 - ibpA b3687 0.49 -2.6 3,865,233 - ibpA b3687 0.49 +0.6 3,865,240 + ibpA b3687 0.50 -0.7 3,865,241 + ibpA b3687 0.50 -0.7 3,865,244 - ibpA b3687 0.51 -2.9 3,865,244 - ibpA b3687 0.51 -0.1 3,865,253 + ibpA b3687 0.53 -1.0 3,865,253 - ibpA b3687 0.53 -0.5
Or see this region's nucleotide sequence