Strain Fitness in Escherichia coli BW25113 around b3514

Experiment: T7_phage 0.0015625 MOI TC1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	T7_phage 0.0015625 MOI TC1
3,657,448	+	mdtE	b3513	0.17	-1.1
3,657,453	+	mdtE	b3513	0.17	-0.7
3,657,456	-	mdtE	b3513	0.17	+0.1
3,657,456	-	mdtE	b3513	0.17	-0.4
3,657,463	+	mdtE	b3513	0.18	+0.8
3,657,552	+	mdtE	b3513	0.26	-0.0
3,657,552	+	mdtE	b3513	0.26	+0.4
3,657,560	-	mdtE	b3513	0.26	-1.4
3,657,560	-	mdtE	b3513	0.26	-0.9
3,657,560	-	mdtE	b3513	0.26	+0.0
3,657,560	-	mdtE	b3513	0.26	-0.7
3,657,604	-	mdtE	b3513	0.30	+0.1
3,657,604	-	mdtE	b3513	0.30	+1.1
3,657,646	-	mdtE	b3513	0.34	+0.1
3,657,674	-	mdtE	b3513	0.36	-0.8
3,657,707	+	mdtE	b3513	0.39	-1.3
3,657,853	+	mdtE	b3513	0.52	-1.5
3,657,853	+	mdtE	b3513	0.52	-0.4
3,657,853	+	mdtE	b3513	0.52	+0.5
3,657,908	-	mdtE	b3513	0.56	+0.8
3,657,908	-	mdtE	b3513	0.56	-0.1
3,657,919	+	mdtE	b3513	0.57	+0.2
3,657,967	+	mdtE	b3513	0.61	+0.9
3,657,967	+	mdtE	b3513	0.61	+0.2
3,657,967	+	mdtE	b3513	0.61	+1.0
3,657,967	+	mdtE	b3513	0.61	-1.0
3,657,967	+	mdtE	b3513	0.61	+1.4
3,657,975	-	mdtE	b3513	0.62	+0.4
3,657,975	-	mdtE	b3513	0.62	-0.2
3,657,975	-	mdtE	b3513	0.62	-2.1
3,658,002	-	mdtE	b3513	0.65	+0.1
3,658,005	+	mdtE	b3513	0.65	-0.3
3,658,049	-	mdtE	b3513	0.69	-1.4
3,658,049	-	mdtE	b3513	0.69	-0.5
3,658,110	+	mdtE	b3513	0.74	-0.5
3,658,129	-	mdtE	b3513	0.75	-0.3
3,658,129	-	mdtE	b3513	0.75	+0.2
3,658,131	-	mdtE	b3513	0.76	-0.3
3,658,133	-	mdtE	b3513	0.76	-0.7
3,658,133	-	mdtE	b3513	0.76	+0.1
3,658,191	+	mdtE	b3513	0.81	+0.3
3,658,191	+	mdtE	b3513	0.81	-0.7
3,658,209	-	mdtE	b3513	0.82	+1.4
3,658,259	+	mdtE	b3513	0.87	+1.1
3,658,284	+	mdtE	b3513	0.89	+0.2
3,658,417	+				+0.5
3,658,420	+				+0.2
3,658,423	-				-1.1
3,658,428	-				-0.8
3,658,428	-				+0.6
3,658,515	+				-0.2
3,658,578	+				+2.5
3,658,578	+				+0.4
3,658,609	-				-0.1
3,658,623	-				+0.8
3,658,623	-				-1.5
3,658,639	+				+2.1
3,658,639	+				+0.0
3,658,645	-				+0.1
3,658,656	+				+2.1
3,658,656	+				-0.7
3,658,670	-				+0.5
3,658,692	+				-0.7
3,658,692	+				-1.1
3,658,791	-	mdtF	b3514	0.11	-0.2
3,658,800	+	mdtF	b3514	0.12	-2.2
3,658,829	+	mdtF	b3514	0.13	-0.2
3,658,837	+	mdtF	b3514	0.13	+0.3
3,658,887	-	mdtF	b3514	0.14	+1.9
3,658,889	+	mdtF	b3514	0.15	-0.3
3,658,904	+	mdtF	b3514	0.15	+0.1
3,658,904	+	mdtF	b3514	0.15	+0.4
3,658,904	-	mdtF	b3514	0.15	-0.6
3,658,904	-	mdtF	b3514	0.15	-1.2
3,658,906	+	mdtF	b3514	0.15	-0.5
3,658,929	+	mdtF	b3514	0.16	-1.3
3,658,929	+	mdtF	b3514	0.16	-1.9
3,658,934	+	mdtF	b3514	0.16	-0.2
3,658,991	+	mdtF	b3514	0.18	+0.5
3,659,034	-	mdtF	b3514	0.19	+0.1
3,659,096	-	mdtF	b3514	0.21	-0.1
3,659,105	+	mdtF	b3514	0.21	+1.7
3,659,105	+	mdtF	b3514	0.21	+1.7
3,659,148	-	mdtF	b3514	0.23	+1.0
3,659,148	-	mdtF	b3514	0.23	+2.2
3,659,237	+	mdtF	b3514	0.26	-0.2
3,659,237	+	mdtF	b3514	0.26	-0.6
3,659,237	+	mdtF	b3514	0.26	+0.1
3,659,274	+	mdtF	b3514	0.27	+0.6
3,659,274	+	mdtF	b3514	0.27	+0.6
3,659,274	+	mdtF	b3514	0.27	+0.6
3,659,313	-	mdtF	b3514	0.28	+1.4
3,659,313	-	mdtF	b3514	0.28	-1.0
3,659,350	-	mdtF	b3514	0.29	+1.0
3,659,350	-	mdtF	b3514	0.29	+1.7
3,659,360	-	mdtF	b3514	0.30	+0.7
3,659,364	-	mdtF	b3514	0.30	-1.4
3,659,471	-	mdtF	b3514	0.33	-0.6
3,659,639	-	mdtF	b3514	0.39	+0.3
3,659,644	+	mdtF	b3514	0.39	+0.5
3,659,644	+	mdtF	b3514	0.39	-0.5
3,659,657	-	mdtF	b3514	0.39	+1.4
3,659,662	+	mdtF	b3514	0.39	-0.1
3,659,675	+	mdtF	b3514	0.40	-0.8
3,659,755	-	mdtF	b3514	0.42	-0.5
3,659,826	-	mdtF	b3514	0.45	-0.0
3,659,902	+	mdtF	b3514	0.47	+0.6
3,659,902	+	mdtF	b3514	0.47	-1.1
3,659,910	-	mdtF	b3514	0.47	+0.2
3,659,915	-	mdtF	b3514	0.47	-1.0
3,659,922	+	mdtF	b3514	0.48	-0.1
3,660,005	+	mdtF	b3514	0.50	+0.7
3,660,013	-	mdtF	b3514	0.51	+1.8
3,660,013	-	mdtF	b3514	0.51	+1.3
3,660,016	-	mdtF	b3514	0.51	-0.2
3,660,016	-	mdtF	b3514	0.51	-1.4
3,660,033	-	mdtF	b3514	0.51	-0.7
3,660,035	+	mdtF	b3514	0.51	+0.3
3,660,043	-	mdtF	b3514	0.52	+1.9
3,660,046	+	mdtF	b3514	0.52	+0.7
3,660,058	-	mdtF	b3514	0.52	+0.3
3,660,126	+	mdtF	b3514	0.54	+0.5
3,660,131	+	mdtF	b3514	0.54	-0.9
3,660,167	-	mdtF	b3514	0.56	+2.2
3,660,167	-	mdtF	b3514	0.56	+0.7
3,660,179	-	mdtF	b3514	0.56	-0.1
3,660,252	-	mdtF	b3514	0.58	-1.6
3,660,252	-	mdtF	b3514	0.58	+1.8
3,660,265	-	mdtF	b3514	0.59	+2.7
3,660,265	-	mdtF	b3514	0.59	+2.1
3,660,303	+	mdtF	b3514	0.60	-0.5
3,660,311	+	mdtF	b3514	0.60	+0.9
3,660,311	+	mdtF	b3514	0.60	-0.5
3,660,319	+	mdtF	b3514	0.60	+0.1
3,660,319	+	mdtF	b3514	0.60	-0.5
3,660,319	+	mdtF	b3514	0.60	+2.0
3,660,321	+	mdtF	b3514	0.61	+1.4
3,660,327	-	mdtF	b3514	0.61	-0.6
3,660,332	+	mdtF	b3514	0.61	+1.4
3,660,415	+	mdtF	b3514	0.64	+0.3
3,660,415	+	mdtF	b3514	0.64	+0.3
3,660,454	+	mdtF	b3514	0.65	-0.2
3,660,491	+	mdtF	b3514	0.66	+0.9
3,660,491	+	mdtF	b3514	0.66	+2.0
3,660,497	+	mdtF	b3514	0.66	-0.4
3,660,498	-	mdtF	b3514	0.66	-0.8
3,660,659	+	mdtF	b3514	0.71	-0.1
3,660,659	+	mdtF	b3514	0.71	+0.1
3,660,784	+	mdtF	b3514	0.75	-1.3
3,660,784	+	mdtF	b3514	0.75	+1.2
3,660,799	-	mdtF	b3514	0.76	+2.0
3,660,802	-	mdtF	b3514	0.76	+0.3
3,660,804	-	mdtF	b3514	0.76	-0.5
3,660,824	-	mdtF	b3514	0.77	-0.2
3,660,884	+	mdtF	b3514	0.79	+1.2
3,660,964	-	mdtF	b3514	0.81	-0.9
3,660,979	-	mdtF	b3514	0.82	+1.5
3,660,979	-	mdtF	b3514	0.82	-2.7
3,661,011	+	mdtF	b3514	0.83	+2.1
3,661,024	+	mdtF	b3514	0.83	-0.3
3,661,041	+	mdtF	b3514	0.84	+2.2
3,661,049	-	mdtF	b3514	0.84	-0.1
3,661,101	+	mdtF	b3514	0.86	+0.3
3,661,101	+	mdtF	b3514	0.86	+0.6
3,661,103	+	mdtF	b3514	0.86	+1.2
3,661,109	-	mdtF	b3514	0.86	-0.0
3,661,109	-	mdtF	b3514	0.86	-0.1
3,661,109	-	mdtF	b3514	0.86	+1.5
3,661,109	-	mdtF	b3514	0.86	+1.7
3,661,117	-	mdtF	b3514	0.86	-0.1
3,661,162	+	mdtF	b3514	0.88	-0.4
3,661,182	-	mdtF	b3514	0.88	-0.5
3,661,373	-				-0.4
3,661,426	+				+0.5
3,661,426	+				-1.7
3,661,446	+				+1.5
3,661,565	-				-0.0
3,661,565	-				-1.3
3,661,567	+				-0.8
3,661,567	+				-1.0
3,661,570	-				-1.7
3,661,634	+				-3.1
3,661,634	+				-0.2
3,661,650	+				+0.4
3,661,716	-				+0.1
3,661,716	-				+0.2
3,661,750	+				+1.0
3,661,866	-				-2.2
3,661,870	+				-2.5
3,661,929	+				+1.3
3,661,929	+				+2.0
3,661,934	+				+0.3
3,661,937	-				+0.6
3,661,938	+				+0.7
3,661,960	+				+0.2
3,661,996	+	gadW	b3515	0.11	+0.2
3,661,998	-	gadW	b3515	0.12	-0.3
3,661,998	-	gadW	b3515	0.12	-3.1
3,662,001	-	gadW	b3515	0.12	+2.4
3,662,006	-	gadW	b3515	0.13	-1.4
3,662,006	-	gadW	b3515	0.13	-0.6
3,662,006	-	gadW	b3515	0.13	+0.2
3,662,009	-	gadW	b3515	0.13	-0.5
3,662,009	-	gadW	b3515	0.13	-1.7
3,662,011	-	gadW	b3515	0.13	-0.6
3,662,011	-	gadW	b3515	0.13	+0.1
3,662,025	+	gadW	b3515	0.15	+0.8
3,662,030	-	gadW	b3515	0.16	-2.6
3,662,030	-	gadW	b3515	0.16	+0.4
3,662,033	-	gadW	b3515	0.16	-1.0
3,662,035	-	gadW	b3515	0.17	-1.6
3,662,038	-	gadW	b3515	0.17	+0.5
3,662,067	+	gadW	b3515	0.21	+2.2
3,662,070	-	gadW	b3515	0.22	-1.1
3,662,070	-	gadW	b3515	0.22	+0.5
3,662,077	+	gadW	b3515	0.22	+4.3
3,662,077	+	gadW	b3515	0.22	-0.9
3,662,111	+	gadW	b3515	0.27	+1.1
3,662,115	+	gadW	b3515	0.28	+1.5
3,662,115	+	gadW	b3515	0.28	+1.2
3,662,125	+	gadW	b3515	0.29	+1.9
3,662,149	-	gadW	b3515	0.32	+0.0
3,662,154	-	gadW	b3515	0.33	-1.4
3,662,154	-	gadW	b3515	0.33	+0.6
3,662,154	-	gadW	b3515	0.33	-0.3
3,662,154	-	gadW	b3515	0.33	-1.3
3,662,158	-	gadW	b3515	0.34	-0.1
3,662,158	-	gadW	b3515	0.34	+0.1
3,662,169	+	gadW	b3515	0.35	+0.7
3,662,171	+	gadW	b3515	0.35	+1.9
3,662,171	+	gadW	b3515	0.35	+2.0
3,662,187	-	gadW	b3515	0.38	-0.6
3,662,197	+	gadW	b3515	0.39	+0.3
3,662,205	+	gadW	b3515	0.40	+0.5
3,662,213	-	gadW	b3515	0.41	-0.0
3,662,235	+	gadW	b3515	0.44	-0.2
3,662,243	-	gadW	b3515	0.45	+0.0
3,662,243	-	gadW	b3515	0.45	-0.9
3,662,243	-	gadW	b3515	0.45	-0.4
3,662,297	-	gadW	b3515	0.53	-0.5
3,662,307	+	gadW	b3515	0.54	-0.1
3,662,308	+	gadW	b3515	0.54	-0.2
3,662,308	+	gadW	b3515	0.54	-0.2
3,662,310	+	gadW	b3515	0.54	+1.0
3,662,311	-	gadW	b3515	0.55	-0.8
3,662,318	+	gadW	b3515	0.56	-0.3
3,662,323	-	gadW	b3515	0.56	-0.3
3,662,330	+	gadW	b3515	0.57	-1.5
3,662,330	+	gadW	b3515	0.57	-0.2
3,662,332	-	gadW	b3515	0.57	+0.6
3,662,332	-	gadW	b3515	0.57	-0.4
3,662,370	+	gadW	b3515	0.63	+1.1
3,662,371	+	gadW	b3515	0.63	+0.8
3,662,371	+	gadW	b3515	0.63	-0.4
3,662,382	+	gadW	b3515	0.64	+2.0
3,662,382	+	gadW	b3515	0.64	+2.4
3,662,390	-	gadW	b3515	0.65	-0.2
3,662,390	-	gadW	b3515	0.65	-0.8
3,662,390	-	gadW	b3515	0.65	-0.5
3,662,390	-	gadW	b3515	0.65	+0.2
3,662,390	-	gadW	b3515	0.65	+1.2
3,662,390	-	gadW	b3515	0.65	-2.7
3,662,390	-	gadW	b3515	0.65	-2.2
3,662,390	-	gadW	b3515	0.65	-0.7
3,662,408	+	gadW	b3515	0.68	+1.7
3,662,408	+	gadW	b3515	0.68	+0.8
3,662,408	+	gadW	b3515	0.68	-0.0
3,662,410	-	gadW	b3515	0.68	+1.9
3,662,410	-	gadW	b3515	0.68	+2.9
3,662,422	+	gadW	b3515	0.70	+2.2
3,662,430	-	gadW	b3515	0.71	-1.1
3,662,462	+	gadW	b3515	0.75	-0.7
3,662,462	+	gadW	b3515	0.75	+0.9
3,662,479	-	gadW	b3515	0.78	+1.3
3,662,484	+	gadW	b3515	0.78	-0.6
3,662,484	+	gadW	b3515	0.78	+1.0
3,662,486	+	gadW	b3515	0.79	-1.0
3,662,489	-	gadW	b3515	0.79	+2.9
3,662,489	-	gadW	b3515	0.79	+2.7
3,662,491	+	gadW	b3515	0.79	-0.1
3,662,494	-	gadW	b3515	0.80	-0.1
3,662,500	+	gadW	b3515	0.81	+2.2
3,662,503	-	gadW	b3515	0.81	+1.3
3,662,503	-	gadW	b3515	0.81	-0.1
3,662,508	+	gadW	b3515	0.82	+0.7
3,662,508	-	gadW	b3515	0.82	+2.9
3,662,510	+	gadW	b3515	0.82	+0.2
3,662,510	+	gadW	b3515	0.82	+0.2
3,662,548	+	gadW	b3515	0.87	-0.3